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40 巻, 6 号
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吉武 裕1986 年 40 巻 6 号 p. 867-875
発行日: 1986/02/28
公開日: 2009/02/17
ジャーナル フリーThe present study discusses the usefulness of Lactate Threshold (LT) as a health indicator in carrying out an exercise program for middle-aged and elderly women.
The subjects were 16 middle-aged and elderly women aged 38 to 58 years (mean±SD=41±7 years).
In order to 1) measure LT and Onset of Blood Lactate Accumulation (OBLA), 2) study the change of sympathodrenal responsea in relation to changes of cardiovascular and metabolic responses, and 3) examine changes of sympathoadrenal, cardiovascular and metabolic responses during 60mins of exercise corresponding to LT, submaximal exercise tests were performed by the subjects.
The results obtained were as follows:
1) Heart rate (HR) and systolic blood pressure (SBP) at LT were 120±14 beats/min and 144±24mmHg, whereas at OBLA 153±12 beats/min and 171±20mmHg, respectively.
2) Plasma noradrenaline significantly increased above the HR level of 150 beats/min. And a significant relationship was found among the noradrenaline, SBP, pressure-rate product and lactic acid (LA).
3) During 60mins of exercise corresponding to LT, LA attained a peak level of 2.01±0.93mmol/l at 10min, and thereafter gradually decreased. On the contrary, there was a progressive increase in plasma free fatty acid and glycerol indicating an increase in lipolysis. Noradrenaline and adrenaline increased 50% and 90% respectively above the resting levels at 60min, at which time values of HR and SBP were 126±21 beats/min and 134±23mmHg, respectively.
In conclusion, it was suggested that LT is a superior indicator in terms of safety and efficiency for exercise prescriptions for middle-aged elderly women.抄録全体を表示PDF形式でダウンロード (1099K) -
熊江 隆, 菅原 和夫, 町田 和彦, 島岡 章1986 年 40 巻 6 号 p. 876-884
発行日: 1986/02/28
公開日: 2009/02/17
ジャーナル フリーThe method of luminol-dependent chemiluminescence (LC method) is one of the measurement methods of the neutrophilic phagocytosis, which has been reported to give results similar to other previous methods and has good reproducibility.
We have succeeded in making a simultaneous multiple measurement system using the technique of LC method and also reported some basic findings on the measurement method of serum opsonic activity.
1. To introduce a one-step separation procedure of neutrophil from whole blood, the lag time from drawing blood to starting measurement is shortened.
2. A remodeled Lumiphotometer TD 4000 (Labo Science Co. ltd.) is used to measure the luminescence. Using a digitizer, each value of measured luminescence (represented in voltage) is fed into the computer. Data of individual samples is printed out in the form of figures; the starting point is the time of adding a neutrophil suspension into the opsonized particles suspension, a horizontal axis means the elapsed time and a vertical axis means the intensity of luminescence.
3. The simultaneous multiple measurement system in this report also exhibits an excellent property of reproducibility. In the region of maximum luminescence, coefficient of variation is a mere 2 or 3% in all cases in this report.
4. According to the results of the simultaneous measurement using a standard serum and same neutrophil, we recognize that the luminescence patterns are characteristic of the particle species.
5. For the purpose of producing the samples graded by the activity of the thermolabile opsonic activator, inactivated serum (56°C, 30min) and normal serum are mixed in specified proportions. From the results of the simultaneous measurement of these samples of plural particles, we can assume that the heat-inactivation effect on the opsonic activity is different from the particle species.抄録全体を表示PDF形式でダウンロード (1070K) -
今木 雅英, 三好 保, 吉村 武, 大谷 信弘1986 年 40 巻 6 号 p. 885-891
発行日: 1986/02/28
公開日: 2009/02/17
ジャーナル フリーThe effects of dietary intake of sugar on serum GOT, GPT, AL-P and LDH activity were examined in 4 young healthy volunteers. The experiment lasted 35 days (usual diets 1; 7 days, experimental diets; 21 days, usual diets 2; 7 days). The composition of the experimental diets was rather high in sugar (low protein low fat), and their serum enzyme activity was measured at intervals.
The results obtained were as follows.:
Serum GOT activity in all subjects increased on experimental diets and the mean activity was approximately 1.7 times the initial level after 21 days in experimental diets. Changes in serum GPT activity were far larger than changes in serum GOT activity. Serum GPT activity in all subjects increased and the mean was about twofold after 21 days in experimental diets. Serum LDH activity showed no significant changes.
It is possible to conclude from the results that serum GOT and GPT activity were affected by high sugar diets.抄録全体を表示PDF形式でダウンロード (682K) -
諏訪湖底質中の栄養塩類の分布特性とその挙動河野 行雄1986 年 40 巻 6 号 p. 892-902
発行日: 1986/02/28
公開日: 2009/02/17
ジャーナル フリーDuring the period from May 1978 to March 1983, research was conducted on the features of horizontal and vertical distribution of nutrients contained in the sediment of Lake Suwa. Laboratory experiments were also conducted to reveal the behavior of the nutrients in the lake sediment. The following are the findings:
Total phosphorus and nitrogen contents were found to be horizontally distributed in the surface sediment almost uniformly throughout the lake except for the estuary and carp-cultivating areas. Their constituent values are represented by the measurements taken in the surface sediment at the center of the lake. The values of total phosphorus and nitrogen contained in the surface sediment at the center of the lake were 3.7-4.0mgP/g dry wt. and 5.0-5.5mgN/g dry wt., respectively.
Total phosphorus contents had rapidly increased during the period from 1972 to 1978. This is considered to have been caused by an increase in the phosphorus load flowing into the lake and its property to accumulate itself. Therefore, the amount of phosphorus contained in the lake sediment is expected to keep increasing in the future in proportion to the load of phosphorus flowing into the lake. However, such property has not been found with the nitrogen contents. It is therefore considered that nitrogen will maintain the current concentration level in the future.
A high correlation was observed between the total phosphorus and the inorganic phosphorus in the surface sediment; 60%-80% of the total phosphorus was found to be inorganic phosphorus. Fe-P contained in the inorganic phosphorus was found to be highly concentrated in the upper layer (0-8cm) of the sediment. Fe-P showed a tendency to decrease in the 0-2cm layer after ten weeks of laboratory experiments as some of it had apparently dissolved out into the upper water layer. However, the amount of Fe-P increased in the 2-6cm layer. From these observations, it became clear that Fe-P had moved up from the lower layer of the sediment to the upper.
A close correlation was found between the density of ammonium in the sediment and the temperature of the sediment. The relationship between the speed at which ammonium is generated and the temperature of the sediment is shown by the following formula:
GNH4-N=0.018exp0.153T+0.427抄録全体を表示PDF形式でダウンロード (1300K) -
坂本 峰至1986 年 40 巻 6 号 p. 903-909
発行日: 1986/02/28
公開日: 2009/02/17
ジャーナル フリーThe effect of dietary lipids on the toxicity of methylmercury (MeHg) was investigated in experimental MeHg poisoning of rats.
Three groups of weanling rats, with 8 to 12 animals in each group, were fed on vitamin E-deficient diet containing 10% beef tallow, cod liver oil or corn oil respectively for 5 weeks and the amount of plasma TBA (Thiobarbituric acid)-reactants, expired ethane and pentane and the level of oxidative hemolysis were measured every week. After the 5 weeks of feeding, the animals were subcutaneously injected with 10mg/kg of MeHg per day for consecutive 5 days. Body weight and crossing phenomenon of hind limbs were checked every day and the amounts of expired ethane and pentane were also measured every 3 days. Results obtained are as follows.
1. At the end of 5 weeks on the diets, the plasma level of TBA-reactants, the amounts of expired ethane and pentane and the level of oxidative hemolysis were significantly higher in the groups fed cod liver oil or corn oil than in the group fed beef tallow, indicating that the lipid peroxidation might be stimulated and the erythrocyte membrane might be weakened in the rats fed vitamin E-deficient diets containing much polyunsaturated fatty acids.
2. Both the latent period for the crossing phenomenon of hind limbs and the survival period after the dose of MeHg were shorter in the groups fed cod liver oil or corn oil than in the group fed beef tallow, suggesting that the former groups might be more susceptible to the toxicity of MeHg than the latter.
3. The amount of ethane or pentane decreased for several days after initial injection of MeHg, and thereafter remarkably increased in agreement with the appearance of the manifest signs of MeHg poisoning in either groups of rats, suggesting that increased production of these hydrocarbons might be a response to the impairment of the defense mechanism against lipid peroxidation by MeHg poisoning rather than MeHg itself.抄録全体を表示PDF形式でダウンロード (844K)
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