Nonoccluded viruses (NOVs) derived from different cells and occluded virus (OV) of nuclear polyhedrosis viruses (NPVs) were compared serologically with cross-neutralization tests. In plaque reduction methods, anti-NOV serum against
Xestia c-nigrum NPV T-3 derived from
Spodoptera frugiperda cells neutralized NOV of
X. c-nigrum NPV T-3 and both NOVs of
Bombyx mori NPV K-4 derived from S.P.C. Bm 36 cells and
B. mori hemolymph. Anti-NOV serum against
B. mori NPV K-4 derived from S.P.C. Bm 36 cells neutralized both NOVs of
X. c-nigrum NPV T-3 derived from
S. frugiperda cells and S.P.C. Bm 36 cells and both NOVs of
B. mori NPV K-4 derived from cell cultures and hemolymph. Anti-NOV serum against
B. mori NPV derived from hemolymph neutralized both NOVs of
B. mori NPV K-4 derived from hemolymph and cell cultures and NOV of
X. c-nigrum NPV T-3 derived from
S. frugiperda cells. These NOVs derived from different cells were neutralized by either homologous and heterologous combination of anti-NOV sera. The results revealed that NOVs of these NPVs were antigenically closely related. While anti-OV serum against
B. mori NPV specially neutralized only homologous virus and anti-NOV serum of
X. c-nigrum NPV T-3 was ineffective for the neutralization of OV of
B. mori NPV. However, both anti-NOV sera against
B. mori NPV derived from cell cultures and hemolymph neutralized OV of
B. mori NPV slightly. The results revealed that NOV of
X. c-nigrum NPV and OV of
B. mori NPV were antigenically different, however, NOVs of
B. mori NPV share a common antigen for infection of OV to cells.
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