The common maturation sequence of the nucleopolyhedrosis viruses (NPV) of
E. subflava and
D. japonica in the fat body cells was as follows: (1) nucleocapsids protruding irregularly from the surface of agglomerated chromatin; (2) regular stacking array of nucleocapsids associated with membranes constructed
de novo; (3) random release of nucleocapsids from the cluster to the nuclear matrix; (4) envelopment of nucleocapsids with a membrane; (5) incipient attachment of polyhedron protein with outer membrane of the enveloped nucleocapsids; (6) progressive deposition of polyhedron protein between two or three enveloped nucleocapsids in close proximity; (7) incorporation of enveloped nucleocapsids into the polyhedron in a haphazard manner; and (8) mature polyhedron covered with polyhedron membrane. The two NPVs were different in the number of nucleocapsids enveloped within a membrane. In case of
D. japonica, 1 to 9 nucleocapsids were enveloped and the other, it was mostly 2 nucleocapsids. No unenveloped nucleocapsid was incorporated in polyhedron protein, although empty envelope or enveloped nucleocapsid was incorporated. During the above morphogenesis and virogenesis, round dark spots associated with chromatin were observed. Sheath-like structures appeared from the virogenic stromata (agglomerated chromatin). Furthermore, long striated structure and fibrillar material structure were observed in the infected nuclei. Morphology of the fibrillar material were distinguished between line periodicity structure and fibrous structure: the former was observed within nuclei connected with polyhedron protein, the latter was observed within both sides of nuclei and cytoplasm.
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