The pathogenicity of two
Verticillium lecanii isolates (MG-Vl-18 isolated from whitefly,
Trialeurodes vaporariorum, and MG-Vl-45 from cotton aphid,
Aphis gossypii) was investigated by inoculation experiments. Both MG-Vl-18 and MG-Vl-45 were pathogenic to
T. vaporariorum, A. gossypii and
Myzus persicae, but host specificities were observed. MG-Vl-18 was more pathogenic to larvae and adults of
T. vaporariorum than MG-Vl-45. The pathogenic difference was especially noticeable in the larval stage at concentrations of 10
4-10
6conidia/ml. The pathogenicity of MG-Vl-45 was stronger than that of MG-Vl-18 to
A. gossypii (apterous adults) and
M. persicae (apterous and alate adults). The mortalities caused by the two isolates were almost the same (96-100%) at high concentrations (10
7, 10
8conidia/ml), but in the case of MG-Vl-18, the time to the death was longer than for other isolates. At lower concentrations (10
6, 10
5, 10
4conidia/ml), the mortalities at 9 days posttreatment for MG-Vl-45 were higher than those for MG-Vl-18. The sizes of conidia and blastospores were different between MG-Vl-18 and MG-Vl-45. MG-Vl-45 produced large conidia and blastospores with dimensions of 8.3×2.5μm (length×width) and 13.1×2.7μm compared to MG-Vl-18 with dimensions of 3.4×1.3μm and 5.5×2.7μm, respectively. The mycelial growth of MG-Vl-18 was faster than that of MG-Vl-45.
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