Two spotted spider mites,
Tetranychus urticae Koch the most important pests of strawberry, have developed resistance to chemical pesticides. We investigated the effects of high carbon dioxide atmospheres on two-spotted spider mites and strawberry plants just before transplanting. Percent mortality of adult female mites, young eggs (24 h after laying), and old eggs (48–72 h) exposed to 60% CO
2 at different temperatures (25, 30, and 35°C) was evaluated over time. Mortality of all spider mite stages increased with exposure duration at each temperature, and the time required to achieve 100% mortality decreased as temperature increased. Exposure to 60% CO2 at 30°C for 16 h was 100% lethal to every developmental stage tested. Furthermore, 24 h of 60% CO
2 treatment did not induce external damage or adversely affect flowering of the primary flower clusters on strawberry plants. In a large-scale test of elevated-CO
2 atmospheres, the two spotted spider mite population was greatly suppressed relative to untreated fields, and strawberry plants remained healthy. Thus, our findings suggested that CO
2 treatment could be used to propagate spider-mite-free plants in strawberry nurseries and enhance strawberry integrated pest management systems.
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