This paper describes the O
2-dependent control of the reactivity of nitrogen oxide species for the production of biologically important nitrated and nitrosated compounds. In this study, the effects of O
2 on the reactivity of NO, NO
2, and ONOO
−/ONOOH for nitration of tyrosine (Tyr) and nitrosation of glutathione (GSH) and morpholine (MOR) were examined. NO produced
S-nitrosoglutathione (GSNO) and
N-nitrosomorpholine (NMOR) through the formation of N
2O
3 under aerobic conditions, and NO
2 produced 3-nitrotyrosine (3-NO
2Tyr), GSNO, and NMOR. Transnitrosation from GSNO to MOR was observed only in the presence of O
2. Although preformed ONOO
−/ONOOH produced all the products under aerobic conditions, the formation of 3-NO
2Tyr and GSNO was markedly reduced and the formation of NMOR was enhanced under anaerobic conditions. The reactivity of the CO
2 adduct of ONOO
− was similarly dependent on O
2. 3-NO
2Tyr was produced effectively by reaction with ONOO
−/ONOOH at the O
2 concentration of 270 μ
M and by reaction with its CO
2 adduct at O
2 concentrations greater than 5 μ
M. Generation of ·OH from ONOO
−/ONOOH was suppressed under anaerobic conditions. The reactivity of ONOO
−/ONOOH and ·OH generation from ONOO
− were reversibly controlled by the O
2 concentration.
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