Inflammatory cytokine interleukin-8 (IL-8) and reactive oxygen species (ROS) overexpressed in the gastric mucosa when exposed to
Helicobacter pylori, defined as a class I carcinogen. Moreover, infection with
H. pylori leads to morphological changes in co-cultured cells known as hummingbird phenomenon along with increased motility. Resveratrol, a highly abundant polyphenol in red grapes, has shown anti-inflammatory, anti-cancer, cardioprotective and neuroprotective activities. However, the effect of resveratrol in
H. pylori-infected cells has not been investigated. The present study was, therefore, aimed to evaluate the effect of resveratrol on the induction of IL-8, ROS and hummingbird morphology in
H. pylori-infected gastric epithelial cells. The non-toxic concentration of resveratrol for both
H. pylori and epithelial cells was determined by brucella broth dilution method and DNA fragmentation assay. The non-toxic resveratrol (≤100 μ
M) treatment did not demonstrate any inhibitory effect against
H. pylori adhesion to gastric epithelial cells. However, preincubation of the cells with 75 and 100 μ
M of resveratrol significantly (
p<0.05 and
p<0.01 respectively) inhibited the secretion of IL-8 from
H. pylori-infected cells. In addition, resveratrol pretreatment at 1—100 μ
M suppressed
H. pylori-induced ROS generation in a concentration dependent manner. Moreover,
H. pylori-initiated morphological changes were markedly blocked by resveratrol. Hence, resveratrol can be considered as a potential candidate against various
H. pylori related gastric pathogenic processes.
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