The present experiment was done to elucidate the dynamics of
Bacillus thuringiensis (
B.t.) in the agroecosystem from microbial ecological viewpoint. Two kinds of
B.t. preparations (AF101 and var.
thuringiensis) were sprayed on the surface of mulberry fields (A; alluvium layer loam, B; ciluvium volcanic ash soil clayloam) with different properties of soil and the number of
B.t. was estimated. There was some difference in dynamics between strain AF101 and var.
thuringiensis. Cell number of strain AF101 decreased from 10
5/g (dry weight of soil) to 10
4/g 1 month after the spray, whereas that of var.
thuringiensis began to decrease gradually 3∼4 months after the spray. Both strains persisted relatively long period at the level of 10
3/g, which was the limiting concentration for detection. The numbers of inherent
Bacillus in soil, concurrently preformed with that of
B.t., were 10
5/g∼10
6/g in summer (May-September) and 10
4/g∼10
5/g in winter (November-February). The proportion in number of sprayed
B.t. to inherent
Bacillus in soil (R value) was 20∼40% immediately after the spray of
B.t. preparation, however, R value lowered to about 10% when the number of
B.t. decreased to 10
3/g. And it remained unchanged for a relatively long period (12∼16 months).
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