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Volume 47, Issue 2
Special Issue on Optical Measuring Technology Feasible for Medical and Biological Applications
Displaying 1-8 of 8 articles from this issue
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Special Issue on Optical Measuring Technology Feasible for Medical and Biological Applications
Special Issue
Laser Review
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Yukihiro OZAKI2019 Volume 47 Issue 2 Pages 78-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSBiomedical spectroscopy has greatly attributed to the innovation of laser in last 60 years. Although developments in optics and detectors brought huge contributions into molecular spectroscopy as well, the laser technology was the largest driving force for the molecular spectroscopy to break through the various difficulties especially in the biomedical applications. In this preface of the present special issue, the history of laser is briefly mentioned along with the footsteps of fluorescence, infrared, Raman and near-infrared spectroscopies.View full abstractDownload PDF (560K) -
Tatsuyuki YAMAMOTO, Hemanth NOOTHALAPATI, Keita IWASAKI2019 Volume 47 Issue 2 Pages 80-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSIn this review, historical background along with some important studies that led to the development of medical spectroscopy in Japan has been introduced. Great interest among researchers for medical applications of spectroscopy began with the development of Laser Raman spectroscopy which was then followed by the progression of FT-IR spectroscopy. Medical spectroscopy field took a quantum leap when spectrophotometers were combined with microscopes which enabled researchers to investigate at sub-cellular resolutions and acquire large number of spectra. This resulted in rapid development of suitable chemometric approaches. Some recent examples on medical applications of Raman spectroscopy and some routinely used chemometric methods such as SVD, PCA and MCR-ALS have been discussed.View full abstractDownload PDF (1243K) -
Daisuke MIYATA, Takakazu NAKABAYASHI, Shin-ichi MORITA2019 Volume 47 Issue 2 Pages 85-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSRecently it became possible to measure Raman spectra of a single live cell using a standard Raman microscope. Research on live cells using Raman microscopes (Bio-Raman research) has attracted many researchers. This review introduces remarkable bio-Raman studies based on the background.View full abstractDownload PDF (1228K) -
Yusuke OSHIMA, Noriko SAGAWA, Takahiro HIRATSUKA, Tuyoshi ETOH, Ma ...2019 Volume 47 Issue 2 Pages 89-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSFluorescence microscopic techniques are widely used in studies for biology and medicine as a powerful tool to visualize morphology and distribution of target molecules in cells and tissues. Laser scanning confocal microscopy enables to obtain optical sectioned high contrast images of biological samples with fluorescent labeling. Multiphoton excitation microscopy is a promising technique for intravital imaging in both basic biology and clinical medicine. Multiphoton excitation is a nonlinear optical phenomenon based on simultaneous absorption of two or more photons by irradiation of ultra-short pulse laser. In particular, nonlinear optical imaging technique has make it possible to analyze deep portions of tissues. Furthermore, second harmonic generation, a nonlinear optical process, is also useful for label-free imaging of collagen in biological specimen. Here we performed intravital imaging to reveal the mechanism of chemotherapy induced alopecia, one of the side effect in anticancer drugs, using multiphoton excitation microscopy in mice. In the result, we observed alterations in the vascular structure and the hair papillae of subcutaneous tissue treated with cyclophosphamide. The technical development in multiphoton excitation microscopy implies a practical tool not only for bioimaging analysis but also for future clinical applications.View full abstractDownload PDF (1331K) -
Hideaki KANO, Hiroaki YONEYAMA, Rie FUJISAWA, Takumi IWAMURA2019 Volume 47 Issue 2 Pages 94-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSWe have developed two-types of ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopic systems using supercontinuum (SC) light sources. The first system employs an InGaAs image-intensified CCD camera, which enables us to perform single-shot CARS measurement. On the other hand, the second system employs a new laser source based on the master oscillator fiber amplifier (MOFA) configuration, which generates sub-100-ps (85 ps) laser pulses with sub-MHz (0.82 MHz) repetition rate. Owing to the high peak power and high repetition rate, the exposure time for CARS imaging of polymer beads was significantly shortened to be less than 1 ms (0.8 ms), which was mainly limited by the readout time of the CCD camera. Using the second system, CARS spectroscopic imaging was performed for living cells.View full abstractDownload PDF (1357K) -
Kosuke HASHIMOTO, Yoshinori HARADA, Yasuaki KUMAMOTO, Tetsuro TAKA ...2019 Volume 47 Issue 2 Pages 99-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSTo observe cells and tissues in thei‘r native’ states is a great challenge for researchers in biomedical fields. Development of a non-invasive and non-labeling analytical method for cells and tissues is eagerly anticipated. Raman spectroscopic measurement is a good candidate because it provides information on biomolecules of cells and tissues in their natural conditions. This technique has high potential not only for medical application such as diagnosis but also for biological research. In this paper, we introduce our basic researches into Raman spectroscopic measurement for biomedical applications.View full abstractDownload PDF (1052K) -
Ichiro ISHIMARU, Akira NISHIYAMA2019 Volume 47 Issue 2 Pages 103-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSOur aim is to introduce middle-infrared spectroscopy into such daily life environments as smart toilets or non-invasive glucose sensors. For this purpose, we proposed ultrasonic-assisted middle-infrared spectroscopy to avoid strong water absorptions. By attaching an ultrasonic transducer to liquid cells, ultrasonic standing waves (longitudinal waves) generate an internal reflection plane near the sample surfaces. If we use an ultrasonic transducer whose frequency is 10 MHz, the depth of a generated reflection plane from the sample surfaces is around 100 μm. We can easily make thin film samples inside of moisture samples that are less than 100 μm thick. We also proposed a beam size (φ 6 mm × 20 mm) for a middle-infrared spectrometer that is a kind of spatial phase-shift interferometer. Because we can introduce a low-resolution middle-infrared camera (80×80 pixels) by maintaining wavelength resolutions, we can achieve reasonably-priced sensors for commodities around 100,000 JPY.View full abstractDownload PDF (1052K)
Laser Original
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Hiroko MATSUYOSHI, Akinori TAKETANI, Hidetoshi SATO2019 Volume 47 Issue 2 Pages 107-
Published: 2019
Released on J-STAGE: August 13, 2021
JOURNAL FREE ACCESSRaman spectroscopy technique, which detects molecular vibration information, determines the chemical structure of substances. To explore its application possibilities for diagnosing disorders, we applied it with a ball-lens mounted hollow-fiber Raman probe (BHRP) to detect the differences between control rats and chronic cystitis model rats that were induced as a first step by the frequent intravesical administration of hydrochloric acid (a model of bladder pain syndrome/interstitial cystitis, BPS/IC). We described the difference of bladder conditions between the control and chronic cystitis model animals by the principal component analysis (PCA) of Raman spectra data. Hence, the Raman technique has potential as a diagnostic method of chronic cystitis, BPS/IC, or other bladder inflammatory disorders.View full abstractDownload PDF (899K)
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