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Volume 38 (1975 - 197･･･
- Issue 5 Pages 339-
- Issue 4 Pages 259-
- Issue 3 Pages 171-
- Issue 2 Pages 85-
- Issue 1 Pages 1-
Volume 38, Issue 4
Displaying 1-7 of 7 articles from this issue
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Tohru WATANABE, Young Ki PAIK, Mikio YASUDA1975 Volume 38 Issue 4 Pages 259-274
Published: 1975
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSThe pancreatic islets of domestic fowl were investigated by electron microscope. The following results were obtained.
1. The A islet is composed of a great many A cells and a few D cells. It also contained only a few B cells which are situated mainly along capillaries.
2. On the contrary, the B islet is composed of a great many B cells and a few D cells. It also contained only a few A cells in its periphery.
3. Immature secretory granules within the Golgi complex are almost the same in size, shape and density among the three types of islet cells. Since there are quite distinct differences in these properties of mature secretory granules in the cytoplasm among the three types of islet cells, there may be a difference in the mode of maturation process among the three.
4. These granules are released from the three types of islet cells through a mechanism termed as emiocytosis.View full abstractDownload PDF (10251K) -
Hisao FUJITA, Hiromu MISHIMA, Nagayasu OTSUKA1975 Volume 38 Issue 4 Pages 275-284
Published: 1975
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSFreeze-etching images of the rabbit thyroid were described. The outline of the structure of the follicular epithelial cell in freeze-etching techniques is consistent with that in ultrathin sections. The subapical junctional complex consists of tight and gap junctions. According to the number of strands (5-16) indicating the tight junction and the depth of the tight junction (0.3-1.1μm), this is classified into the “very tight” form of CLAUDE and GOODENOUGH (1973). The number of intramembranous particles per μm2 on the A-face of the lateral as well as the apical plasma membrane is far larger than that on the B-face. The limiting membrane of the reabsorbed colloid droplet shows also a similar pattern to that of the plasma membrane. The capillary endothelial cells show numerous fenestrations whose population density is about 20/μm2 on the endothelial surface except in the parajunctional zone.View full abstractDownload PDF (7180K) -
Hiromasa AMAGASE1975 Volume 38 Issue 4 Pages 285-298
Published: 1975
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSThe ultrastructural changes in the liver cells of guinea pigs induced by the oral administration of PCB were studied by electron microscopy; also electron-microscopic cytochemistry for glucose-6-phosphatase (G-6-Pase) activity was applied.
Proliferation of smooth endoplasmic reticulum (sER) was the most prominent change observed in the liver cells, which remained as long as 90 days after the final administration.
G-6-Pase activity was ultracytochemically demonstrated not only in the rough and smooth endoplasmic reticulum and the nuclear envelope in the liver cells of normal controls, but also in the proliferated sER in the liver cells of PCB-treated animals.
The present investigation revealed that PCB stored in the animal body induced the proliferation of sER in the liver cells for a long time after the cessation of the treatment, and that sER in the liver cells, normally existing or proliferated, always showed positive activity of G-6-Pase.View full abstractDownload PDF (9296K) -
Tanenao ETO, Torao YAMAMOTO, Teruo OMAE1975 Volume 38 Issue 4 Pages 299-306
Published: 1975
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSHypertensive encephalopathy was induced in the rat by clipping one renal artery and contralateral nephrectomy. The possible changes of vascular permeability of the cerebral blood capillaries and venules were investigated by using ferritin as a tracer. The uninephrectomized rats served as controls.
In controls, ferritin was never seen in the basement membranes, within plasmalemmal vesicles on the basal surface of the endothelium, or in endothelial cell junctions of cerebral capillaries and venules up to 180min after the injection. In venules of the brain in rats with hypertensive encephalopathy, a number of ferritin particles appeared in the basement membrane in 60min after the injection. Many plasmalemmal vesicles in the endothelial cells of venules were labeled with ferritin. However, ferritin particles were never found in the endothelial cell junctions.
The results suggested that leakage of macromolecules, such as serum proteins, occurred in venules mainly by increased vesicular transport.View full abstractDownload PDF (5282K) -
Michitoshi OSAKA1975 Volume 38 Issue 4 Pages 307-319
Published: 1975
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSFine structure of the duodenal mucosa in ten human fetuses 4 to 7 months old was studied by light and electron microscopy using Epon-embedded specimens.
Cells containing membrane-bound granules were found both in the connective tissue and in the epithelia and identified to be basal-granulated cells, since they essentially corresponded in their cytoplasmic fine structures to the named cells in the adult. However, their relation to nerves and epithelial elements revealed some primitive features.
In the 4 month fetus, the basal-granulated cells were seen in the lamina propria forming clusters which were often in contact with the basal part of the epithelium. In the 5 month fetus, occurrence of clustered basal-granulated cells in the connective tissue was rare. In the 7 month fetus, most of the basal-granulated cells were distributed singly among other epithelial cells, gaining an apical process which reached the lumen.
The basal-granulated cells in the lamina propria were frequently surrounded by a Schwann-like cell. Profiles of unmyelinated nerve fibers were ample in the space between the Schwann-like cell and the basal-granulated cell.
An Ω-shaped invagination of the basal plasma membrane encompassing granular material was often found in the D type basal-granulated cells in the 7 month fetus. This suggests that the secretion of at least some of the basal-granulated cells starts in the prenatal period by emiocytosis.View full abstractDownload PDF (9567K) -
Sa'di F. AL-SAMARRAI1975 Volume 38 Issue 4 Pages 321-334
Published: 1975
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSVarious methods of preparation were tested and modified in order to establish a proper method of preparing normal and pathological human erythrocytes for scanning electron microscopy. Morphological changes after various preparation techniques, such as echinocytosis, spherocytosis, elliptocytosis and knizocytosis were studied and evaluated statistically. The best method, so far obtained to preserve 99% of normal erythrocytes as biconcave discocytes, was that of venous blood without anticoagulants or acid-citrate-dextrose, washed with physiological saline at 37°C, fixed in 0.75% glutaraldehyde in 0.1M phosphate buffer, pH 7.3 (318 mosmol), postfixed in 1% osmium tetroxide, dehydrated with graded ethanol and amyl acetate, dried with critical point drying method, and coated with carbon and gold.View full abstractDownload PDF (2584K) -
1975 Volume 38 Issue 4 Pages 335-338
Published: 1975
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSDownload PDF (413K)
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