The composition of the adjuvant emulsions was 2.5% squalene, 6% detergents, 0.5% antioxidant – α-tocopherol or β-carotene and 91% water phase. Antioxidant effectivity was testing by determination of peroxide value. α-tocopherol acted as a prooxidant, β-carotene was an effective antioxidant. Effectiveness of rabies vaccine with squalene adjuvant was testing on mice. Adjuvanted vaccine with β-carotene was compared to vaccine without antioxidant and induced a slower but prolonged immunity response with protective levels of rabies antibodies (0.5 IU/mL).