Extractions of the protein from fresh frozen algae with some reagents and change of the releasing of protein with autolysis were studied. On the protein extraction, algae was ground sufficiently with a little amount of reagent and sea sand in mortar and soaked in the reagent. The supernatant obtained from this treatment after centrifugation at 4000r.p.m. for 20 minutes was used to determine, the nitrogen extracted. These results are shown in Table 1. Also, the results of changes of releasing of protein with autolysis of algae are shown in Figure 1. Considering from the results shown in the Table 1, NaOH seems most extractable of all the reagents employed, the extracted put being 50% of total nitrogen of algae. On the other hand, only a little protein-nitrogen was extracted. With autolysis for 50 hours, 7096 of total nitrogen of algae and 50% of total protein nitrogen became soluble. Figure 2 also shows the results of change of protein releasing with autolysis by paper electrophertic diagrams. Figure 3 presents the electron-microscopic photography of observation of these process of autolysis.

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The influence of the stannous tartrate added diet feeding for the growth and maintenance of rats are examined.
Less than 10mg% stannous tartrate in the diet, no influence. But from 55 to 110mg% stannous tartrate in the diet, caused serious interference, motorial hindrance on the hind legs, and at last someone fell dead.

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Examined the digestibility of food components, and recognized that there is no influence to the digestibility of each component of diet by adding the stannous tartrate.

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Tin retention in the liver and the kidney of rat are did not detected by the polarographic analysis, when examined the viscera of adult rat breeding in long intervals (14 weeks) with a diet containing 110mg% of stannous tartrate.

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An investigation are attempted about the change of intestinal flora of rat, by the taking of diet containing some stannous tartrate. The intestinal flora, used for this study, are selected of general intestinal flora of rat, such as Escherichia coli, Aerobacter, Streptococci and Clostridium type bacteria. The result are shown as follows.
(1) In the case of breeding with the diet containing a stannous tartrate from 2mg% to 10mg%, the intestinal flora of rat did not occur any changes, however, according to the increasing of tin content (from 55mg% to 110mg% as stannous tartrate), a number of aerobe are decreased and the growth of Escherichia coli type bacteria are suffered.
(2) In the medium containing stannous tartrate from 55mg% to 110mg%, the frequency of appearance of aerobe are decreased, and one of Escherichia coli type bacteria are markedly decreased also, compared with control medium.

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