This experiment revealed that growth of young rats were improved by increasing the dietary protein level, whereas the fat level, unlike the protein level, showed no marked effect on the growth.
Liver thiobarbituric acid value increased accompanying the increase of the dietary protein level from 0 to 18%, the fat level 0 to 10%, in each case when the protein or fat level was kept constant.
At levels exceeding 18% for protein and 10% for fat, liver thiobarbituric acid value did not show higher value (s).
From the results obtained, it was concluded that liver homogenates prepared from an animal attaining good growth give high TBA value(s) which may be due to the high biochemical metabolism of the animal.

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Effects of whole-body ⁶⁰Co-γ irradiation on body weight gain, weight of thymus, liver, spleen, kidney and testis; liver thiobarbituric acid value(s), liver fat level and content of the experimental rats were examined, and self-restoration from the radiation damages were also observed for 30 days duration after the irradiation.
Six hundred roentgen of cobalt-60 gamma ray irradiation caused the reduction of diet consumption for several days after the irradiation. The loss of appetite resulted the decrease of body weight of the exposed animals. A marked fall of liver thiobarbituric acid value(s) of the irradiated rats were observed at the next day of the irradiation, and the restoration from the radiation damages led to give high liver thiobarbituric acid value(s). The irradiation caused the reduction of weights of thymus, spleen, and the increase of liver. The influences of the irradiation could be observed in thymus, spleen, even at the last day of this experimental period. The radiation effects were not obvious in testis and kidney for the first 10 days after the irradiation, but the effects became significant at the end of the observation.

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Effects of dietary protein level on the influences of 800 roentgen of whole-body ⁶⁰Co-γ irradiation were investigated using following criteria: body weight gain and its ratio, diet consumption and its efficiency ratio, protein intake and its efficiency, urinary nitrogen excretion, liver fat level, weights of organs, and mortality.
The results obtained show that the influences of the irradiation are relatively small in rats reared with the diet in which the dietary protein level is lying between 12 to 18%, but in the cases the protein level is below 12% and exceed 18%, the radiation damages are significant.

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Effects of dietary protein from three kinds of protein sources on the influences of 800 roentgen of whole-body ⁶⁰Co-γ irradiation against rats were compared using the same criteria as used in Report 6. The three kinds of protein sources were milk casein, soybean oil meal, and mixture of corn flour and soybean oil meal (as protein, 1:3). Dietary protein level of the three test diets in this experiment was 12% each.
This experiment showed that the influences of the irradiation were the most significant in rats fed the mixture-protein diet and the influences were the smallest in the animals fed the soybean-protein diet.
Concentration of carcass fat in the non-irradiated controls was highest in the animals fed mixture-protein diet, and there was almost no difference in the fat concentration between the both animals fed the casein diet and fed the soybean-protein diet.
Concentration of unsaturated fatty acids of carcass fat in the non-irradiated animals fed the mixture-protein diet were the highest, and were the lowest in the animals administered the soybean protein diet. The concentration of unsaturated fatty acids in the animals fed the casein diet was a little higher than that of the animals fed the soybean-protein diet.
Although growth of the animals fed the mixture-protein diet was the best among the animals of 3 groups administered the 3 diets during pre-irradiation period, the radiation damages were the most significant in this group.
From the results obtained, it is concluded that source of protein is as important factor as dietary protein level in prevention of the radiation damages, and in also concluded that there is difficulties to suspect the radiation susceptibility from the growth of the experimental animals.

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It has been already reported by us that the growth of the implanted tumor is remarkablly affected by the restriction of essential amino acids. From our amino acid imbalance studies, it has been found that the restriction of phenylalanine causes the returdation of tumor without any undesireble side effect against the host. Such effect of phenylalanine deficient diet has also been found to be accelerated by the restriction of tyrosine. The phenylalanine hydroxylase activity of liver decreased remarkablly in the tumor bearing animal. Foretheremore, it has also been confirmed that the restriction of an essential amino acid causes the elongation of the doubling time of tumor cell.
From the experimental facts mentioned above, the effectiveness of amino acid imbalance diet has been clinically investigated in all types of leukemia and other malignant diseases. The patients have been treated initially with low phenylalanine diet and then recently with low phenylalanine-tyrosine diet. The combined restriction therapy of phenylalanine and tyrosine seemed to be more effective than the single restriction of phenylalanine. Phenylalanine-tyrosine limiting milk according to our prescription has been used as the main nitrogen source of the diet. The dosis of phenylalanine and of tyrosine supplied daily were 1.8g. respectively for adult and were 1.1g. for infant. Such phenylalanine dosis as mentioned above exceeds slightly the lowest essential dosis of it.
Up to now, 22 patients were treated with this amino acid imbalance diet for one month to tow and half years, without any side effect. The patients treated with this diet were as follows; 11 cases of acute leukemia including 5 infants, 6 cases of chronic leukemia and 5 cases having solid malignant tumor. Single application of this diet not so effective except few successful cases. The clinical responce against chemotherapy, however, was remarkably improved with this diet therapy and long term remission was obtained. One case of malignant lymphoma has been well controled for long time with this diet therapy only.

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In despite of the improved procedures of the occult-blood tests of feces, the dieticians frequently meet some difficulties in preparing a menu for the patients, because it is very important to control diets prior to these tests. Some of the vegetables, which are boiled more than thirty minutes, are proved to be available for this purpose. The foodstuffs, which might cause a false positive reaction unspecificially in the tests, should be excluded from this menu. In this experiment, the authors reexamined the influences of cooking for occult-blood tests, using the 68 vegetables and 28 meats, and attempted to magnify the materials in the list of control-diets.
Four methods are employed as occult-blood tests; Hematest B, Benzidine test (Adler), Pyramidone method (Adler) and Guajac method (Deen). The occult-blood test, in vitro, are carried out on each food material before and after cooking. The same procedures are done on the feces of healthy volunteers with the same materials. The number of false positive vegetables in all vegetables is markedly reduced after three minutes' boiling. The same result of decrease is obtained on the feces with boiled vegetables, being more prominent in degree than on fresh vegetables only. These results, however, are not obtained on the meats.
The present data shows that the control of diets and the method of cooking previously employed for the occult-blood tests are too much exaggerated.

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