With a day-by-day comparative examination of the growth of buckwheat sprouts-grown in the dark and seedling-grown in the light, 1-ascorbic acid and rutin content were determined, the acid by the indophenol method and rutin by the paperchromatographic method. Theamount of 1-ascorbic acid in the seedlings (35.8-48.5mg%) was found more than that in the sprouts (11.1.-36.9mg%). And the amount of rutin in the seedlings (147-208mg%) about twice that in the sprouts (77-92mg%). Then the rutin in the sprouts was isolated and identified by elementary analysis, absorption spectrum with infrared rays and other methods of examining properties.

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Studies on the loss of ascorbic acid during the manufacturing process and preservation of Orange Marmalade were carried out.
As the result of the experiment, it was recognized that most of ascorbic acid which was containd in a fruit as the material of sweet marmalade was lost, although ascorbic acid in the bitter marmalade was remaind. The loss may be depend on dissolving of ascorbic acid into water in the procass of washing. The loss of ascorbic acid the enriched marmalade was 48 percent when preserved it for a week at room temperature (25°C-30°C)

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It has been previously observed by Nagase and Fujita that riboflavin synthesis by intestinal bacteria was markedly promoted when the diet was supplemented with cellulose. It is very interesting, therefore, to research what changes would occur on the bacteria in the intestine by cellulose supplementation. It was experimented under the next conditions.
The experimental subject was a 20-year old, healthy male, weight in 62kg and 173cm in height. The three kinds of experimental diets as shown in Table I were used, i. e., ordinary, meat and vegetable diets.
As source of cellulose, cellulose powder and Toyo filter paper No. 7 were used. Each diet was supplied for 7 days by the next order, ordinary diet, vegetable diet, vegetable diet supplemented with cellulose powder, ordinary diet, meat diet, meat diet supplemented with filter paper and finally ordinary diet. The filter paper was ground in a mortar until it became mushy and was added to polished rice at a concentration of 2 per cent of the total diet and cooked.
The measurements of various bacterial counts was published in the previous report.
1. The amount of fecal riboflavin had a tendency to rise in case of the vegetable diet, but when the vegetable diet was supplemented with cellulose powder fecal riboflavin content was not increased, from which it is supposed that the effect of cellulose powder as cellulose was questionable. However, the amount of riboflavin in feces was found to decrease as changed to meat diet, and to rise by addition of mushy filter paper to this diet.
2. Total bacterial count and coli form count hardly changed. Clostridium welchii count had a tendency to decrease by addition of cellulose. A switch of an ordinary to a vegetable diet resulted in small increase in Lactobacilli, and also when the mushed filter paper was added to the meat diet, the increase of Lactobacilli was observed.
But in both Aerobacter and anaerobic bacilli the results were not so clearly.

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In compliance with the request of a certain college for nutritional administration of athletes staying together in the camp for training of boating, we have conducted a nutritional survey and administered their daily diets. In this report, the results obtained from biometrical measurements, observations of degree of physical fitness, basal metabolism values, the energy consumption of rowing and the energy consumed throughout the day are summarized as follows:
(1) Biometrically the athletes were in exellent codition in respects of their boody length and breadth. However, as subcutaneous fat values were low, they are consequently assigned to the balanced muscular body type.
(2) The strength of the back and vital capacity were also eminent and function of circulation was likwise above normal.
(3) The basal metabolic rate was more than 10% higher than the Japanese standard, and was specific as usually seen in case of heavy muscular workers.
(4) The energy consumption values attained in performance of many different ways of rowing ranged from eight to twenty-eight times as much as the basal metabolism. Therefore it is considered that in concern of the energy consumption rate, they belong to the top group surpassing all other athletes except those taking instaneous athletics such as sprinters whose energy values are the highest.
(5) The energy consumption per day amounts to 4900 cal. which is exceptionally high exceeding energy consumption value of other kinds of athletics.

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Average serum iron level of the male was 109±23.9γ% and of the female was 101±22.9γ%.
On the diurnal variation of serum iron content in healthy adults, it seemed that serum iron level started rising at 6 a. m., and the peak was reached at 2 p. m., and began to fall gradually reaching the lowest at 2 a. m., which indicated that there was large variation during the 24 hours.

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After the administration of ferrum reductum, serum iron level was elevated and reached maximam value in 4 hours. Then gradual decrease was seen, and coming back to the pretest level and thereafter no rising trend was seen for 32 hours.
To comfirm the ferrum reductum administration would always cause the rise of serum iron level, ferrum reductum was given during night, and the rise was recognized, indicating that ferrum reductum was practically absorbed.

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