Since the discovery of a High voltage-paper electrophoresis method for the separation of the amino acids by Michl in 1951, it has been recognized as a quite effective method for the analysis of the amino acids.
In this report, a very simple apparatus for this purpose was used and satisfactory results was obtained on the quantitative analysis of lysine, histidine and arginine.
The advantage of this method is; by using rather long filter paper on the apparatus, a clear diagram of separated amino acids can be obtained in a short time.

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Of quantitative Analysis methods for the amino acids which had been isolated in various ways, a ninhydrin method is known as the most common and effective one.
In this report, as a continuous experiment of our recent paper, a colorimetric ninhydrin method is used for the quantitive analysis of lysine, arginine and histidine, after the separation by Highvoltage-paper electrophoresis.

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Arbino rats were fed a casein diet, zein diet and non-protein diet, and the contents of gastrointestinal tracts were analyzed for 70% ethanol soluble nitrogen, and 4 free amino acids as described by Nasset (1955).
It was obserbed that all 4 amino acids were present in almost same rate throughout the test meals and the segments of intestine.
Therefore, these results suggested that endogenous amino acids were secreted into intestinal tracts during digestion and absorption of diets.

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Compare Antioxidant action of New Antioxidant; IC (isopropyl citrate), EMQ (6-ethoxy-1. 2-dihydro 2. 2. 4. trimethyl quinoline) and UPO 88 with BHA and BHT.
The following results were obtained; UPO 88 was qreatest antioxidant activity.
Also Compare Antioxidant action to alcohol extracted four fraction of EMQ by the paperchromatographic analysis.

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The thiamine actions were investigated about thiamine anhydride sulfoxide (TAO) and thiamine anhydride sulfone (TAO₂) which were obtained by oxidating thiamine anhydride with H₂O₂. It was found that though 500λ of TAO and TAO₂ were respectively administrated to the rats which were feeded with thiamine deficient diet, each substance did not show the thiamine action and that LD₅₀ of TAO₂ was lowe than that of thiamine.

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Both thiamine anhydride (TA) and thiamine anhydride sulfone (TAO₂) obtained by oxidation of TA did not show the biological activities. It was studied whether that survival rate was low when the TAO₂ action was tested. When 500λ of TAO₂ in the presence of 5λ of B₁ was administrated to rats, TAO₂ did not show antimetabolic action.

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Bio-potency of vitamin A of fish liver oil was estimated from their maleic value according to Ames et al. As the results, maleic value of raw liver oil and distilled fish liver oil were about 35, and relative bio-potency about 65% for all-trans isomer of vitamin A. But from view point of effect of distillation and freshness of liver oil, relative bio-potency of vitamin A of fish liver oil was considered to be more than 90%. Isomerization of synthetic vitamin A in oil was observed under storage and radiation of ultra violet light, and its bio-potency recognized to show sometimes below 75%. It was observed in vivo that the type of vitamin A storaged in, liver was hardly influenced by distribution of isomer of vitamin A adminstered, as well as by ratio of distribution of vitamin A isomer in feed. Decrease of maleic value in liver in vitro was found under addition of “slow reacting isomer”. Isomerization of vitamin A on the wall of intestine was not remarkable. Relation between bio-potency of vitamin A shown by growth of rat and maleic value of vitamin A source was exsamined, and only within the specific range of dosing of vitamin A the above relation was observed.

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