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Volume 28 (1970)
- Issue 6 Pages 225-
- Issue 5 Pages 167-
- Issue 4 Pages 119-
- Issue 3 Pages 89-
- Issue 2 Pages 51-
- Issue 1 Pages 1-
Volume 28, Issue 2
Displaying 1-7 of 7 articles from this issue
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[in Japanese]1970 Volume 28 Issue 2 Pages 51-52
Published: 1970
Released on J-STAGE: October 29, 2010
JOURNAL FREE ACCESSDownload PDF (2384K) -
Haruyo Yamazaki, Ryokuero Suzue, Hiroo Sanada, Yoko Nakashima, Shoji K ...1970 Volume 28 Issue 2 Pages 53-56
Published: 1970
Released on J-STAGE: October 29, 2010
JOURNAL FREE ACCESSIt has been known that ultraviolet (UV) irradiation causes mutation and death in many organisms, and that UV-inactivated organisms can be photoreactivated.
The present paper describes the photoreactivation in vitamin B1-requiring mutants of E. coli B. Three kinds of mutants, which require respectively pyrimidine, thiazole and VB1, were isolated. The cells of wild strain and three mutants were irradiated with UV light of 255mμ at the rate of 0.194mW/cm2. After irradiation the cells were immediately exposed to photo reactivating (PR) light of 320-420mμ for 15 to 60 minutes.
The survival curves of Th-, B1- mutants were similar to that of the wild strain, but Pm- mutant was extremely sensitive to UV irradiation.
When cells were irradiated by UV light at a dose of 40×0.194mW/cm2, the wild strain was almost completely reactivated by exposure to PR light for 15 minutes, and Th-, B1- mutants were reactivated in 60 minutes. However, in Pm- mutant, the phenomenon of photoreactivation was not observed.
After irradiation of UV light at a dose of 80×0.194mW/cm2, only the wild strain was reactivated by exposure to PR light for 60 minutes. On the other hand, none of three mutants could be reactivated.
The present results indicated that in the wild strain and VB1-requiring mutants of E. coli B, UV-induced damage was repaired by exposure to PR light, although the photoreactivating ability of mutants was lower than that of the wild strain. It was concluded that the photoreactivating activity differs by the kind of mutants and the degree of UV damage.View full abstractDownload PDF (3652K) -
Haruyo Yamazaki, Hiroo Sanada, Ryokuero Suzue, Yoko Nakashima, Shoji K ...1970 Volume 28 Issue 2 Pages 57-60
Published: 1970
Released on J-STAGE: October 29, 2010
JOURNAL FREE ACCESSPhotoreactivation of ultraviolet (UV) damage has been demonstrated in many bacteria, plants and animals.
In the previous paper, we reported on the phenomenon of photoreactivation in vitamin B1 requiring mutants of E. coli B. The present study was performed to clarify the mechanism of photoreactivation.
E. coli DNA labeled with 3H-thymidine was irradiated with UV light of 255mμ. In the presence of visible light, the irradiated DNA was incubated with enzyme which was extracted from E. coli B. The reaction was terminated by addition of trichloroacetic acid. The precipitated DNA was hydrolyzed with formic acid, paper chromatographed and the radioactivity of each fraction was counted.
As the result of this experiment, a new radioactive peak of thymine dimer with Rf value 0.24 was observed.
However, about 30% of the dimers were split off from irradiated DNA by photoreactivating treatment.
It was concluded that photoreactivating enzyme exists in E. coli B, and UV-induced thymine dimers in DNA are eliminated by the enzyme in the presence of light.View full abstractDownload PDF (4019K) -
Investigation of Nutrition Survey and Food SurveyNobuko Wakahara, Misako Kuwahata1970 Volume 28 Issue 2 Pages 61-68
Published: 1970
Released on J-STAGE: October 29, 2010
JOURNAL FREE ACCESSIn July, 1968, the author surveyed the status of nutrition in two different regions in Kagoshima, that Furue-cho, Kanoya city (a seaside region) and Honmata, Togo-cho, Satsumagun (a mountainous region), and made a study of regional difference by the correlation during in each nutritive elements, each food group intake, and between each nutritive elements and each food group intake.
The results are as follows; the status of nutrition intake is not recognized the significance for regional difference in the average value, and though it reached almost the level, there was a great difference in the status of its distribution, and it showed much homely difference in same region.
In the status of food intake, the significance for average values of two regions are find in mostly food group, and especially there was a great difference both in sorts and in quantity of caloric sources and proteinic sources.
In the seaside region, the proteinic sources food is mainly fish, pulses and cereals.
In the mountainous region, animal proteinic food of every kind are taken and it recognized remarkable mutual relation between protein and animal protein.View full abstractDownload PDF (8335K) -
Nutritional Analysis of Obesity among Children during Lodging Together for TrainingYukie Wada1970 Volume 28 Issue 2 Pages 69-76
Published: 1970
Released on J-STAGE: October 29, 2010
JOURNAL FREE ACCESSWe organized a class of 18 obese children during summer vacation for the purpose of studying the relation between nutrition and physical development, especially centering in their way of spending time, nutrition intake and consequent weight change.
(1) Their heat value intake per day was reduced to 1, 600-1, 300 calories, and as a new method of satisfying the resultant hunger feeling of children, they were served with soup before meals, which proved to be effective.
(2) The balance of nutrition intake, excepting heat value and sugar, in its nutritions quality, was kept more adequately during the term of their lodging together here for training than before, and despite of the decrease in their weight, children were in a good state of health.
(3) The ratio of the constituents of the nourishment taken in here indicated that the degree of dependence of their calorie intake upon cereals before they joined this training was higher in each group, and animal protein intake during the term of their lodging together here for training was in much higher proportion than the attainable standard in the 45th year of Showa.
(4) Food, except cereals, was taken in larger quantity during their staying here for training, and effective difference was recognized in the case of vegetable food. As for animal food, individual difference was great, which was not recognized in their intake of cereals.
(5) Their weight changed during their staying here for training, and group II and group III respectively showed the decrease of 4.0% and 4.6%.View full abstractDownload PDF (8894K) -
Chika Fujimura1970 Volume 28 Issue 2 Pages 77-83
Published: 1970
Released on J-STAGE: October 29, 2010
JOURNAL FREE ACCESSThis paper is to report the results investigated the following subjects to the junior class students who major in food and nutrition in the women's university. The investigations were done for five years from 1963 to '67.
The subjects are: 1. The result of the nutritive investigation. 2. The condition of the student's diet, the degree of consciousness of their physical condition, and the investigation of the condition of the students with or without taking breakfast in the recent growing trend toward the latter case.View full abstractDownload PDF (8545K) -
(1) Result of local investigationM. Hamano, H. Kuwabara, S. Kozima, S. Kisi1970 Volume 28 Issue 2 Pages 84-88
Published: 1970
Released on J-STAGE: October 29, 2010
JOURNAL FREE ACCESSAccording to the economic growth and development of communication and production, farm villages near large cities are gradually urbanized and their dietary life is also remarkably changing with the change of their living conditions.
As a method of eliminating the difference of nutrition of the nation, the government is aiming at the activity of home dietists. For this purpose, the origin and real state of the change and difference and the method of instruction for amelioration must be investigated. Hence the authors studied the problem on housewives of 99 families chosen at random from 6 localities within the jurisdiction of the Huzioka health centre.
As the result, their community social feeling was common and uniform. Almost all of them have a desire to study. Therefore it is a problem how to make places of study and organize the group activities.
In order to aim at the completeness of popularization, it is important to make them to prepare the place of participation by themselves, because housewives have their own living conditions.View full abstractDownload PDF (5434K)
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