Eighty blind school pupils in a boarding house, 6 to 25 years of age, were grouped into two. Forty pupils of the experimental group were given the supplementary diet of each 40g non-fat milk solid, vitamin B₁ and cerebrolysin tablets per day for six months.
The other 40 pupils were grouped as the control and given placebos. The dietary records of the boarding house were obtained for ten consecutive days in the summer and the autumn to calculate the nutritive values of the diets.
Physical examinations (height, weight, girth of the chest and sitting height) and analyses of blood constituents (protein, vitamin A, total cholesterol, and alkaline phosphatase activity in serum) were conducted prior to, during and at the end of the experiment.
The following results were concluded:
I) The nutrient intake of the boarding house was relatively good compared with mean values for Japanese people. However, vitamin intakes were deficient compared with their recomended allowance.
II) No significant effects on the physical measurements were detected which could be attributed to the supplements except the mean weight of the experimental group.
III) Serum protein and vitamin A concentrations did not show significant difference in both groups and those values kept normal levels.
IV) Greater percent of the lower level of total serum cholesterol was found at the experimented group compared with the placebo treated group.
V) Alkaline phosphatase activity in serum was signifficantly higher at the experimented group than the other.

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The use of the short-time urine samples instead of 24hrs. urine is of very important significance for the estimation of the nutritional status of the groups or the individuals by biochemical methods, and in this study the analysis and the discussion were made about it.
The urine samples were collected from 35 healthy women, 21 to 49 years of age, and the whole day urine was divided into 4 parts, morning (6-12), afternoon (12-18), night (18-24), and sleeping-time (0-6) urines.
The urinary excretions of thiamine, riboflavin and N′-methylnicotinamide (MNA), as well as nitrogen and creatinine were determined, and interrelationship among them in each timed urine and also correlation between the intake and excretion were examined.
The results were:
(1) About the excretions of thiamine, riboflavin and MNA, 24-hour urine primarily correlated with sleeping-time urine.
(2) Between the intakes and excretions of thiamine the highest correlation was found in the 24-hour urine, and secondarily in sleeping-time urine. Riboflavin also showed the similar tendency.
(3) When expressed the urinary excretions of thiamine and riboflavin either per hour basis or per gram of creatinine basis, there was observed little difference in the coefficient of variation nor in the correlation between the timed urine and the whole day urine.

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