Juices of 46 green vegetables, 3 potatoes, 2 mushrooms and 15 fruits were investigated for their scavenging activities against superoxide anion radical (O
2) by the luminescence method with xanthine-xanthine oxidase system. IC
50 measurements of all the samples were performed on five solutions ranging in concentration from 0.01 to 100% (w/v) of each sample juice.
1) Juices from yellow and red peppers showed the most remarkable scavenging activities among all the samples, followed by juice of tomatoes and cherry tomatoes. Carrot contains a large quantity of β-carotene, but showed a weak O
2 radical scavenging activity compared to other vegetables. Juices in this study contained only the water-soluble components, leaving the oil-soluble components in the samples. The weak O
2 radical scavenging activity of carrot may be due to absence of the oil-soluble components.
2) Chinese green leaf vegetable (chingensai) and asparagus demonstrated the strongest O
2 radical scavenging activity among green and yellow vegetables. Other colored vegetables such as turnip, cabbage, onion, ginger root, garlic, radish-daikon and Chinese cabbage demonstrated strong O
2 radical scavenging activities. These results may be attributable to the total action of polyphenol, vitamin C and other compounds in water-soluble fractions.
3) In the botany classification as edible parts, “fruit vegetables in solanaceas”, “root and herbage vegetables in cruelferace”, “root vegetables in nymphaeceate” and “herbal vegetables in lillaceae” strongly scavenged O
2 radical. Two types of mushroom showed an activity similar to those of turnip and cabbage. Juices of grapefruit, lemon, orange and mandarin orange demonstrated strong activities.
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