1. First molars of C₃H fetal mice were dissected out, pretreated in 30per cent glycerol, frozen rapidly in liquid nitrogen (-195°C), rewarmed rapidly at 37°C. in TYRODE's solution, and then transplanted to the anterior eye chambers or axilla of adult mice. Transplants were considered successful when odontoblasts and ameloblasts differentiated and formed dentin and enamel.
2. Tooth germs frozen without pretreatment in glycerol almost always failed to take on transplantation.
3. Pretreatment with glycerol increased the proportion of successful transplants among frozen tooth germs from 0 to 33per cent in the case of anterior eye chamber transplants and from 4 to 57per cent in tooh germs transplanted to the axilla.
4. Tooth germs that were exposed to glycerol but not frozen showed less damage than glycerol-treated frozen tooth germs. However, the glycerol solution in itself caused some injury under the conditions of this experiment.
5. The group of tooth germs that were neither treated in glycerol nor frozen showed the highest proportion of successful takes, about 85per cent in both the anterior eye chamber and axilla.
6. Under the conditions of this experiment, the axilla proved to be more favorable than the anterior eye chamber as a site for transplantation. No difference was noted in the case of control transplants.

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1. Estrogen treated animals showed an exceptionably well developed blood supply in the pulpal area, but no other significant differences were observed between hormone treated and untreated animals.
2. Tooth germs grown in vitro for periods of up to 10 days were able to establish and were verified to still keep the vitality for normally growing in host animals.

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The sensory nerve supply of the urethra of dog is very notable in general, and in particular, most liberal in the pars prostatica but somewhat less so in the partes praeprostatica, membranacea and cavernosa.
The sensory fibres entering the mucous membrane of the pars praeprostatica end in subepithelial and intraepithelial simple branched terminations in most cases but more rarely in capsulated and uncapsulted small-sized glomerular bodies. The uncapsulated bodies are smaller than the capsulated. Many terminal fibres in the branched terminations show change in size and end searply or bluntly. Small endbodies resembling PACINIan bodies are not rarely found in the submucosa here.
In the pars prostatica, simple branched termiations are often found along the ducts of the prostatic glands. Their terminal fibres often enter the epithelium. Very small-sized PACINIan bodies are found in the submucosa in this part. Most of the sensory fibres running into the mucous membrane here mostly end in branched and less often in corpuscular terminations. These are superior in number as well as in construction to those in the preprostatic part. The end bodies here too mostly resemble the genital nerve bodies Type I, but a fow belong to the Type II of such bodies. Most of the branched terminations are formed intraepithelially and not rarely complexly enough. Their terminal fibres, sometimes rather thick in size, often show conspicuous change in size.
In the pars membranacea urethrae, PACINIan bodies are found in the adventitia covering the m. urethralis and these are often found in groups of 2 or 3 bodies each. The connective tissue lamellae count from 10 to 15 and the incoming single thick sensory fibre ends in a blunt point withouf branching. Very peculiar branched terminations formed by enormously thick sensory fibres as found in the urethra of cat are found in the inner circular smooth muscle layer of the m. urethralis of dog, too. These are well developed in the proximal portion of the part where the muscle layer is in good development but even in the more distal portions where the muscle layer is poorer, similar terminations are not at all absent. These seem to belong to the category of the ABE's so-called prostatic sensory terminations found in the same part in man.
In the pars membranacea, the glomerular and branched terminations formed in the mucous membrane are less well developed, eithar numerically or structurally. Neither PACINIan body nor end bulb have been found here. The glomerular terminations comprise capsulted and uncapsulated ones and lose both in number and in size in the more distal portions. These glomerular terminations are found not rarely in the venous plexus outside the mucous membrane, too.
The branched terminations also grow slowly poorer distalwards, but often I found rather complex branched terminations unexpectedly in the distalmost area. The terminal fibres of these terminations are often thick and show conspicuous change in size and mostly end in the epithelium, but subepithelial branched terminations are not rare either. The terminal fibres showing change in size of the latter very frequently run up into the epithelium, then curve down again into the epithelial basis and end sharply. Branched terminations of other types, i. e., those with branch fibres spreading arborescently toward the epithelium and those of a few branch fibres showing change in size during their winding courses spread out over a wide area in the propria, are found only very scantily.
The sensory supply of the pars cavernosa urethrae of dog is poorer than in man but is far better than in cat. Here, branched terminations are formed, most often intraepithelially, while the glomerular terminations are far larger in number and better in formation than those in the pars membranacea; intraepithelial fibres are often sent out from the latter

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In der vorliegenden Untersuchung wurden Riechdrüsen bei verschiedenen Mammalien, nämlich Kaninchen, Meerschweinchen, Ratten, Pferden, Rindern, Schafen, Schweinen, Hunden, Katzen und Fledermäusen cytologisch, histologisch zum Teil auch histochemisch eingehend studiert. Die ganz frische Riechschleimhaut aus Regio olfactoria der genannten Tiere wurde als kleine Gewebsstücke mit dem LEVIschen, CHAMPYschen Gemisch, ZENKER-Formol, 10% Formol u. a. fixiert, in Paraffin eingebettet, dann in 4-5μ dicke Serienschnitte zerlegt. Für die Darstellung der Mitochondrien wurden die mit LEVIschem und CHAMPYschem Gemisch fixierten Paraffinschnitte mit dem Eisenhämatoxylin nach HEIDENHAIN, Anilinfuchsin-Aurantia nach KULL und Azan gefärbt, diese Präparate wurden auch für verschiedene cytologische Beobachtungen der Riechdrüsen benutzt. Für den Nachweis des Mucins wurden die Perjodsäure-SCHIFFsche Reaktion (PAS) und die BAUERsche Reaktion an den mit ZENKER-Formol fixierten Schnitten appliziert, dabei wurde die Speichelverdauungsprobe für die Unterseheidung des Mucins von dem Glykogen angewandt. Außerdem wurden einfache Thionin- oder Toluidinblaufärbung, Berlinerblau-Reakion für Eisennachweis, SudanIII-Färbung u. a. angestellt. Für die Herstellung der Übersichtspräparate wurden die durch ZENKER-Formol fixierten Schnitte mit Hämatoxylin (HANSEN)-Eosin oder Azan angefärbt.
Bei genannten 10 Säugetierarten sind die Riechdrüsen morphologisch miteinander sehr ähnlich und zeigen keine wesentlichen morphologischen Abweichungen.
Die Gl. olfactoria stellt bei allen untersuchten Tieren eine einfache verästelte tubulöse Drüse dar und erweist sich durch morphologische, färberische und histochemische Beobachtungen als eine Art Schleimdrüse. Sie verteilt sich in der Riechschleimhaut verhältnismäßig dicht und erreicht die tiefste Schicht derselben. Die einzelnen Riechdrüsen teilen sich in einen kurzen intraepithelialen und in einen im Bindegewebe der Riechschleimhaut ziehenden, langen Abschnitt ein; der kurze intraepitheliale Abschnitt durchsetzt in der Regel das Riechepithel senkrecht, stellt zum Teil oder die ganze Länge hindurch den Ausführungsgang der betreffenden Riechdrüse dar, indem er mit einem eigentlichen, einschichtigen Plattenepithel des Ausführungsgangs bekleidet ist; im ersteren Falle dringt der Drüsentubulus aber eine kurze Länge in das Riechepithel hinein (der intraepitheliale Abschnitt des Drüsentubulus). Der lange, im Bindegewebe der Riechschleimhaut ziehende Abschnitt der Riechdrüse vertritt den Drüsentubulus oder -schlauch, nämlich den sezernierenden Abschnitt. Der lange, öfters leicht gewundene Drüsentubulus der Riechdrüse besteht aus großen, dunklen, verschiedentlich gestalteten Drüsenzellen und der schlecht ausgebildeten, sehr schwachen Membrana propria; er teilt sich wieder in einen subepithelialen oder superfizialen Abschnitt, einen mittleren oder Drüsenkörper und einen tieferen oder Drüsengrund ein. Der intraepitheliale Abschnitt stimmt in verschiedenen morphologischen Beschaffenheiten mit dem subepithelialen Abschnitt überein.
Die großen dunklen Drüsenzellen vertreten bei den Riechdrüsen von verschiedenen Säugetieren die gewöhnlichen Drüsenzellen, die lebhafte Schleimsekretion ausüben und sich in verschiednen Sekretionsstadien, Ruhe-, Restitutions- und Stapelstadium, befinden. Sie führen außer den Mitochondrien stets starklichtbrechende orangegelbe Pigmentgranula und homogene hyaline Substanz in wechselnder Menge; die gewöhnlichen Riechdrüsenzellen zeichnen sich bei verschiedenen Säugern durch diese beiden Cytoplasmaeinschlüsse aus.

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In order to study the effects of the cortical hormone of the suprarenal glands on the secretory activity of the gastric peptic cells, hydrocortisone 1mg, 5mg, 10mg per 1kg of the animal's weight was injected subcutaneously 1 time, 5 times and 10 times and, on the other hand, hydrocortisone 10mg/kg or anthranilic acid 10mg/kg was injected for 5 days after the total extirpation of the suprarenal glands. The secretory activity of the gastric peptic cells in each experiment was compared and discussed with those obtained in case of normal animals. The results obtained can be summarized as follows.
1. It is obvious that the cortical hormone has some effect upon the secretory ability of the gastric peptic cells. The fact can be analyzed as in the following cases.
2. The regular and sure effect is to inhibit the discharge of the gastric hormone productin from the gastric surface cells, regardless of the dose injected. Accordingly, as a result of this effect, a small dose of the hormone seems to be inhibitory on the secretory activity of the peptic cells.
3. A large dose of the hormone inhibits the secretory activity of the peptic cells more remarkably than it inhibits the discharge of productin.
4. An intervening dose of the hormone has irregular effects in different cases, it sometimes actively promotes production of secretory granules in the peptic cells and sometimes vacuolization of the granules. The reason for this, it is assumed that the cortical hormone is not a direct stimulant of the secretory activity of the peptic cells but effective on it by the balance with some other substance.
5. As a result of the extirpation of the suprarenal glands and of the injection of 10mg/kg hydrocortisone or 10mg/kg anthranilic acid for 5 days after the extirpation, it is noticed that a promotive effect on the vacuolization of the peptic secretory granules seems to be peculiar to the cortical hormone in addition to its regular and sure effect on productin secretion.

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1. In this study, we have tried to investigate the formation of dental hard tissues, especially amelogenesis, by utilizing a fluorescence microscope. As a result of this study, the secondary fluorescence of the sections stained with acridin orange as fluorochrom shows various and well differentiated fluorescence colors, which proves most effective.
2. The fluorescence microscope used in this study is one combined with LEITZ's camera microscope ‘Panphot’. The source of radiation used was an PHILIPS' high pressure mercury vapor lamp CS. 150, operating at 120 volts and 2.7 ampere. As the filter was designed to remove visible radiation from ultra violet light, 4mm thick blue fluorescence filter BG 12 is used, and as a filter for absorbing the red portion of visible spectrum, the liquid filter filled with 3per cent copper sulphate solution, is used. Ultra violet protective filters inserted into the microscope ocular are those of 2.5mm thick OG 1. Photomicrographs were taken by the one lens reflex camera attached to the microscope. The films used for this purpose are FUJI Color (reversal type, ASA 10), Ektachron (daylight type, ASA 32), and Konicolor (negative, ASA 50).
3. The distributions and the changes of the elements that fluoresce bright orange or bright orange red color in the cytoplasm of each enamel organ cell at each stage will coincide with those of ribonucleic acid (RNA) in the cytoplasm of the same cells (Fig. 1, 2, 3 and 4) The granules in the cytoplasm of the ameloblasts can, to a certain degree, be distinguished by the difference of fluorescence color, it seems. (Fig. 2)
4. By this methode, the secondary fluorescence color of the enamel matrix, especially the one at the formative stage, is similar to that of the granular layer and the deeper zone of keratin layer of the epidermis and both the fluorescence colors change in the same way, according to pH of buffer dye solution changes (Table 1).
5. It has thus been recognized that ‘cuticle-like structure’ in the ameloblasts at the maturation stage shows entirely different fluorescence color from the other portion of cytoplasms (Fig. 3 and 4).
6. It is considered that a fluorescence microscopy is a very usefull method for histological and histochemical studies on amelogenesis. However, it is required that we should be very careful of the interpretation of these findings, and further this fluorescence microscopy must be applied with routine methods, histological and histochemical.

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The ampulla recti of mole in its proximal part being lined by a very thin wall, the nerve fibres running into it are very limited in number and both the AUERBACH's and the MEISSNER's plexus are very poorly developed, the ganglion cells in them being exceedingly scarce. In the more distal part of the ampulla, however, the muscularis as well as the submucosa become better developed and these formations attain the maximal development in the distalmost part of it. Therefore, in the last part the AUERBACH's plexus is powerfully formed and contains large ganglia, and smaller ganglia are otherwise found in the adventitia covered by the m. sphincter ani ext. of striated nature, The MEISSNER's plexus, however, remains rather ill developed here too.
The nerve fibres coming into the lower part of the rectum contain a considerable number of thick myelinated sensory fibres besides numerous fine vegetative fibres. The sensory fibres run through the plexus without coming into contact with the sympathetic ganglion cells and form their terminations in the submucosa or further up in the propria. The larger part of the terminations are of unbranched and simple branched type formed in the propria and most frequently in the connective tissue between the intestinal crypts. The terminal fibres are usually medium-sized and often show change in size in their gently winding courses. Besides, some uncapsulated simple glomerular terminations can be found on rare occasions.
The anal canal of mole extends very long. The proximal two-thirds of it constitutes the zona columnaris ani having longitudinal mucous folds, with both the columnae and the sinus covered under a noncornified stratified flat epithelium and well-developed papillae formed into it out of the propria. Far more numerous sensory fibres come into this zona columnaris than into the lower part of the rectum. Their terminations are of branched type. The terminal fibres show conspicuous change in size during their wavy or looped courses and usually end sharply over a wide terminal area. In this part, however, such intraepithelial fibres and sensory terminations in and near the periproctal glands as found abundantly in this part of man and some other mammals could never be found.
The distal one-third of the anal canal is the zona intermedia devoid of the longitudinal folds. The epithelium is here somewhat thinner than in the zone columnaris above and the papillae are worse developed. The mucous membrane here, however, contains a number of incoming sensory fibres forming branched terminations, which are, however, somewhat simpler in formation than those in the preceding zone. None of the terminal fibres form intraepithelial fibres here either.
The distalmost part of the anal canal forms the zona cutanea ani containing a few hair follicles and is followed by a patch of haired skin covering the anal canal and the lower part of the rectum. This skinny part contains a huge number of sensory fibres, perhaps accountable by the presence of circularly running smooth muscle fibres in the corium and the good development of the papillae from the stratum papillare into the epidermis, giving the skin of this part a close resemblance to that of the outer genitals. The fibres usually terminate in branched terminations and end bulbs formed by very thick sensory fibres. Of course the hair follicle necks are found to contain well-formed sensory terminations as usual.
The branched terminations in this part are found mostly beneath the epidermis. The branch fibres are large- or small-sized and show conspicuous change in size during their winding courses common to such fibres before ending in sharp points. Sometimes very many terminal fibres partake in forming rather complex branched terminations. On rarer occasions, the terminal fibres are found arranged in glomeruli.

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The dorsum linguae is probably the part best supplied with sensory fibres in the whole body, but the distribution and the terminal mode of these fibres are not uniform in all the different types of the lingual papillae.
The sensory terminations found in the filiform papillae comprise uncapsulated glomerular terminations and rather complex branched terminations formed of terminal fibres showing frequent change in size. In the fungiform papillae, the types of terminations are similar to those found in the filiform papillae above, but the glomerular terminations are somewhat less abundant than in the latter. But as the epithelium of these papillae nearly always contains taste-buds and does not show cornification as in the case of that on the filiform papillae, intraepithelial fibres of fine size are found in this epithelium. Beneath the taste-buds, branched terminations formed of thick terminal fibres frequently changing size are found in most cases, and finer intra- and extra-gemmal fibres are sent out from these.
The number of sensory fibres coming into the foliate and the vallate papillae is smaller than of those in the filiform and the fungiform papillae, and these form only a few uncapsulated glomerular terminations but mostly relatively simple branched terminations composed of rather fine terminal fibres. These terminations terminate beneath the epithelium, particularly in relation with the taste-buds. Not rarely, however, branched terminations originating in very thick fibres and rather complex in form are found spread out over large areas. A very large number of vegetative fibres are found also running into these papillae, forming terminal reticula in every part of their stock.
The facies inferior linguae containe only so-called mucous papillae, so that the sensory fibres in its mucous membrane are much less abundant than those on the dorsum linguae. Comparativeiy large papillae are found in the lateral margins and near the median line of the inferior surface, and some sensory fibres are found running into them to form sensory terminations very similar to those in the filiform papillae above in type but simpler in architecture than these, but most of the smaller papillae are devoid of sensory fibres. Taste-budded papillae are not scarce on this side of the tongue either; in these are found sensory terminations of the same type as those in the fungiform papillae but worse developed than the latter. More rarely, some interesting capsulated glomerular terminations resembling the genital nerve bodies Type I were discovered subepithelially in this part.
Such genital body-like capsulated terminations could be found in the propria of the dorsum linguae too. These are usually large in size than those found in the bacies inferior linguae.
Sensory terminations are also found along the ducts of the gl, apicis linguae. These comprise not only rather complex branched terminations composed of branched fibres showing frequent change in size but also uncapsulated glomerular terminaions. A part of their terminal fibres run further into the epithelium of the ducts.
The most interesting fruit of my study reported herein was the discovery of a rather large number of very peculiar complex branched terminations in the submucosa and the lingual muscle tissue of the apex linguae. These terminations are originated in 1 or 2 thick sensory fibres each, which, upon reaching the terminal area rich in specific cell nuclei, branch out into many branch fibres showing frequent change in size in their utterly irregular looped courses and form neurofibrillar expansions during their courses or at their ends. Thus, they resemble the Type I sensory terminations concerned with the blood-pressure falling reflex, but some are more resembling the genital nerve bodies Type III found in the tunica albuginea of the human penis.

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