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Volume 39 (1976)
- Issue 5 Pages 295-
- Issue 4 Pages 207-
- Issue 3 Pages 149-
- Issue 2 Pages 79-
- Issue 1 Pages 1-
Volume 39, Issue 3
Displaying 1-7 of 7 articles from this issue
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Takehiko OKUDA, Takuro OGATA1976 Volume 39 Issue 3 Pages 149-156
Published: 1976
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSCiliated cells were found in the mucosa of the human stomach in three patients. In two cases they occurred in the pyloric mucosa of patients with intestinal metaplasia who were operated on for duodenal ulcer. In the other case, they occurred within a polypoid lesion. They were located in small limited areas.
The cells were densely ciliated, each cilium showing a typical 9+2 fibrilar pattern.
Ciliated cells are not found in normal gastric mucosa and this suggests that they occur only in pathologically altered mucosa.View full abstractDownload PDF (16432K) -
Takuro MURAKAMI, Shozo IRINO1976 Volume 39 Issue 3 Pages 157-163
Published: 1976
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSGlycol methacrylate hardened with 10-15% glycerin showed no charging and gave dark contrast in the scanning electron microscope with an acceleration voltage of 25kV and a specimen current of 1×10-10A. Using human blood cells, it Was shown that this conductively treated resin is a useful mounting substrate to highlight the non-coated microsamples or to obtain their highly contrasted images under the scanning electron microscope. It was also demonstrated that the glycerin-substituted blood cells can be scanned with little shrinkage.View full abstractDownload PDF (9787K) -
Keiichi TANAKA, Akihiro IINO, Tomonori NAGURO1976 Volume 39 Issue 3 Pages 165-175
Published: 1976
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSIon-etching technique on the cracked surface of biological material may give a plastic visualization of intracellular structures under the scanning electron microscope, because membraneous structures in the cell are generally etch-resistant and the cytoplasmic matrix is easily ion-etched.
Mild ion-etching using low voltage was applied to the cells of the pancreas. Nuclear pores were clearly disclosed but were enlarged slightly in the process of etching. Endoplasmic reticulum with ribosomes, Golgi apparatus, mitochondria, some filamentous structures and crystalline inclusions (B cell granules) were also effectively disclosed by ion-etching technique.
It is necessary, however, to compare the etched specimens carefully with non-etched ones to determine whether given structures observed are intrinsic ones or artifacts caused by etching.View full abstractDownload PDF (19562K) -
Shiro KOBAYASHI, Hitoshi OSATAKE, Yuzuru KASHIMA1976 Volume 39 Issue 3 Pages 177-181
Published: 1976
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSAfter direct injection of Mercox into the thyroid parenchyma we obtained the corrosion casts of lymphatics to be observed by scanning electron microscopy.
It seems reasonable to deduce from the following points that these corrosion casts were of the lymphatic system: 1) variable size of columnar vessels, 2) their three-dimensional anastomoses, 3) occurrence of blind endings, 4) V-shaped reliefs on parts of the columns, certainly indicative of valves, 5) bead-like structures formed by closure of valves, 6) depressions and surrounding saw-toothed figures on the surfaces of the casts, probably corresponding to the nuclei and boundaries of endothelial cells.
The method described in this study seems useful in the study of the structures and changes of lymphatics in various organs.View full abstractDownload PDF (5467K) -
Yasumitsu NAKAI, Toru IWASHITA1976 Volume 39 Issue 3 Pages 183-191
Published: 1976
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSCorrelative light and electron microscopy on the same cells of the adrenal gland of the frog, Rana nigromaculata, fixed in glutaraldehyde followed by osmium tetroxide, was done using the adjacent Epon embedded sections.
Electron microscope observation revealed three different types of granule-filled secretory cells; the noradrenaline-storing cells (NA cells) filled with intensely dense and varying shaped granules, the adrenaline-storing cells (A cells) filled with relatively less dense granules and the summer cells (STILLING, 1898) containing very large, round or polygonal granules (0.2-1.3μ in diameter).
Light microscopically, an essential difference could be observed in the affinity to ammoniacal silver solution between NA and A cells. It was clarified that the granules of NA cells stained in black and were clearly distinguishable from the yellow- or brown-stained granules in both A cells and summer cells. This silver method can be applied for the light microscopic identification of the NA cells in the Epon-embedded sections. Furthermore, after immersing the thick sections in toluidine blue or methylene blue, the granules of NA cells showed much stronger affinity to both dyes than those of A cells and became dark blue and occasionally stained greenish blue in methylene blue, while the summer cells became blue and the granules of the A cells stained light blue.View full abstractDownload PDF (7855K) -
Seiki ITO, Shigeru KOBAYASHI1976 Volume 39 Issue 3 Pages 193-202
Published: 1976
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSCells possessing glucagon- and glucagon-like immunoreactivity (GLI) were studied by an indirect immunofluorescence technique in the normal canine gastrointestinal mucosa and pancreas.
Glucagon-immunoreactivity was demonstrated in the basal-granulated cells in the deeper portion of the fundic gland of the stomach as well as in the A cells of the pancreatic islet. GLI-positive basal-granulated cells were found in the fundus of the stomach, jejunum, ileum and colon. None of them were found in the pyloric antrum and duodenum. Careful examination of the immunofluorescence specimens gave us the impression that essentially every GLI-positive cell in the gastric fundus and pancreas contained glucagon.
It was observed that the GLI-positive cells in the jejunum, ileum and colon were open in type reaching the lumen with their tapered luminal process, whereas the glucagon/GLI-positive cells in the stomach were always separated from the lumen by a layer of other epithelial cells so that they were closed in type.
The possible difference in the way of stimulus-reception between the GLI-positive cells in the intestine and glucagon/GLI-positive cells in the stomach was discussed with particular reference to the idea that basal-granulated cells could receive information for their secretory activity not only from the gut lumen but also from the blood side.View full abstractDownload PDF (7769K) -
1976 Volume 39 Issue 3 Pages 203-205
Published: 1976
Released on J-STAGE: February 20, 2009
JOURNAL FREE ACCESSDownload PDF (277K)
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