Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
Online ISSN : 1882-4528
Print ISSN : 0030-1558
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Volume 109, Issue 7-12
Displaying 1-10 of 10 articles from this issue
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  • [title in Japanese]
    [in Japanese]
    1997 Volume 109 Issue 7-12 Pages 125-127
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_125
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  • [title in Japanese]
    [in Japanese]
    1997 Volume 109 Issue 7-12 Pages 129-132
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_129
    JOURNAL FREE ACCESS
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  • Expression of parathyroid-related peptide mRNA in the kidney
    Kunihiko AYA
    1997 Volume 109 Issue 7-12 Pages 133-140
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_133
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    PTHrP mRNA was quantified by RT-PCR and the localization of PTHrP mRNA was determined by in situ hybridization in kidney. During development, the expression of PTHrP mRNA is much higher before 2 weeks of age while kidney is developing. In E16 fetal mice, PTHrP mRNA was strongly expressed in the collecting ducts, urothelium of the pelvis, tubules, the glomeruli and immature elements in the cortex of the developing kidney (nephrogenic zone). Within O-day-old mice, it was dominantly expressed in collecting ducts, the urothelium of the pelvis and nephrogenic zone. These findings suggest that PTHrP plays a role in kidney development as an autocrine/paracrine factor.
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  • The effect of testosterone on the growth of the human chondrosarcoma-derived chondrocytic cell line HCS2/8
    Masayoshi SATO
    1997 Volume 109 Issue 7-12 Pages 141-150
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_141
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    Sex steroids have a great influence on bone maturation in childhood, especially in adolescence. However, the precise mechanisms are not fully understood. Human chondrosarcomaderived chondrocytic cell line HCS2/8 cells were cultured in DMEM culture medium supplemented with 1% fetal calf serum, 1×10-7M testosterone or estradiol for 24 hours in a subconfuluent state. Then the cells were pulse labeled with 3H-thymidine to measure DNA synthesis. In addition, S1 nuclease protection assay of insulin-like growth factor (IGF)-I, -II and IGF-binding protein (IGFBP)-2, -3, -4 and IGFBP-5 mRNA was carried out. DNA synthesis of HCS2/8 cell was enhanced in the presence of testosterone but not in the presence of estradiol. Using S1 nuclease protection assay, testosterone increased expressions of IGF-I, -II, and IGFBP-3mRNA. However, estradiol decreased the expression of IGF-II mRNA but did not change expressions of IGF-I, IGFBP-2, -3, -4 or IGFBP-5 mRNA. In conclusion, the present results indicate that testosterone stimulates the growth of chondrocytes, probably via increased expressions of IGF-I, -II, and IGFBP-3 mRNA.
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  • Physical development of tissue-reacted cationic iron colloid with protein silver and gold chloride
    Takuro MURAKAMI, Wei Dong SU, Luo Jia HONG, Da Xun PIAO, Aiji OHTSUKA, ...
    1997 Volume 109 Issue 7-12 Pages 151-156
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_151
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    Rat brain sections were treated with fine granular cationic iron colloid, which was prepared by boiling ferric chloride with hydrazine hydrate and cacodylic acid (Murakami et al., 1986). The sections were then treated with ferrocyanide and HCl for Prussian blue reaction or with protein silver and gold chloride for physical development. Physical development had an advantage over Prussian blue reaction in that it produces clear and highly contrasted images under a light microscope. Thus, the perineuronal sulfated proteoglycans and the fine processes of the nerve cells were clearly demonstrated by physical development.
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  • A comparative study of obesity and life styles between Japanese and Chinese children
    Zhuan WANG
    1997 Volume 109 Issue 7-12 Pages 157-164
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_157
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    In 1993 and 1994, a questionnaire survey was conducted on elementary school students of fourth and fifth grades living in Shengyang city (620 children), China and in Okayama city (579 children), Japan to determine differences in the degree of obesity and life styles between the children of the two countries. Children whose body mass index (BMI) was 22 or higher were considered obese. The percentage of obese children was 5.7 in Shengyang and 5.4 in Okayama. Multiple logistic regression analysis of dietary patterns and life styles to explain childhood obesity in both countries showed the importance of consuming large amounts of grain products, potatoes, sugar, and oily foods as factors related to childhood obesity. Physical activity has also been shown to be negatively and significantly associated with childhood obesity. Daily intake of energy, carbohydrate, and fat among obese children was significantly greater than those among nonobese children. The length of time spent watching television among the Japanese children was found to contribute to childhood obesity.
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  • A follow up study on preventive measures against occupational low back pain
    BaoLong Liu
    1997 Volume 109 Issue 7-12 Pages 165-172
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_165
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    In order to evaluate the preventive effects of preventive measures against low back pain (LBP) at a worksite which were carried out on the basis of a 10-year-ago study, a follow up study of the same workplace was conducted using the same study method. The preventive measures included improvement of working environment, exercises at work breaks, medical advice to the workers with severe LBP and decreasing overtime work brought by change in the economic situations at enterprises.
    Results were as follows:
    (1) Point, period (a month) prevalence rates and prevalence rate of severe LBP and absence days due to LBP (deducted absence days in a year) were all significantly lower than those of 10 years ago.
    (2) Onset of LBP among young workers were mainly chronic, which is closely related to heavy lifting and constrained working postures such as bending and squatting etc.
    (3) Absence from work due to LBP was related to work years, severity of LBP and level of job satisfaction.
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  • [title in Japanese]
    1997 Volume 109 Issue 7-12 Pages 173-179
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_173
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  • [title in Japanese]
    1997 Volume 109 Issue 7-12 Pages 181-191
    Published: December 25, 1997
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_181
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  • [title in Japanese]
    1997 Volume 109 Issue 7-12 Pages 193-205
    Published: December 25, 1997
    Released on J-STAGE: October 29, 2012
    DOIhttps://doi.org/10.4044/joma1947.109.7-12_193
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