Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
Online ISSN : 1882-4528
Print ISSN : 0030-1558
Volume 85, Issue 5-6
Displaying 1-7 of 7 articles from this issue
  • Tadasuke KONDO, Mamoru HIROHATA, Kenji SEO
    1973 Volume 85 Issue 5-6 Pages 161-164
    Published: June 30, 1973
    Released on J-STAGE: March 30, 2009
    JOURNAL FREE ACCESS
    Direct bilirubins in vivo was fractionated into bilirubin diglucuronide, bilirubin phosphate and bilirubin sulfate. Influence of pH concentration on the absorptions maximum of each azo-pigment was studied. The schift of absorptions maximum was the same in the former two direct bilirubins and the latter moved as nearly as the indirect bilirubin-azo pigment yielded after an addition of methanol. These results was supposed to the difference was based on the structual difference of bilirubin due to the conjugation type of bilirubin.
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  • Mitsuo TAIRA
    1973 Volume 85 Issue 5-6 Pages 165-182
    Published: June 30, 1973
    Released on J-STAGE: March 30, 2009
    JOURNAL FREE ACCESS
    When a dose of actinomycin D was intrathecally injected into higher animals (dogs and cats), these animals experienced generalized seizures after a latent period of several days.
    The critical seizure producing quantity was 0.025mg on the dog. Actinomycin D combines with DNA in the nervous tissue of the brain, this may be the cause of the seizure effect.
    When the same dose (0.025mg) of actinomycinic acid, which has a chemical structure resembling that of actinomycin D, but does not combine with DNA in the tissue, was injected into the cerebro-spinal fluid of the dog, the animals did not show any behavioural change.
    Electroencephalographic studies of the actinomycin D seizure were carried out on the cat. Several days after the administration of actinomycin D, the animal showed the typical generalized seizure discharges. A generalized seizure discharge induced by actinomycin D began with the occurrence of frequent spikes in group in all of recording sites, then the repetition of a spike and wave complex or a polyspike and wave complex followed. The intervals of each spike and wave complex gradually grew longer, and the seizure discharge stopped.
    After the actinomycin D administration, the electroencephalogram sometimes showed remarkable spike discharges occurring in the hippocampal region. They occurred mostly in hippocampus, but to a lesser degree spike discharges occurred in the other regions.
    In the preconvulsive state after the actinomycin D administration, regular waves of 8 cps occasionally appeared simultaneously with the occurrence of myoclonic jerks.
    In the case of the injection of actinomycinic acid into the cat, no significant change occurred on the electroencephalogram.
    However, the injection of DNA into the cerebrospinal fluid, simultaneously performed, with the injection of actinomycin D, did not stop the seizure effect of the latter.
    Methionine sulfoximine, characterized by a seizure effect with a long latent period, was also injected into the cerebro-spinal fluid of the dog. The critical seizure producing quantity was 0.18mg. These two convulsants with long latent period, the actinomycin D and the methionine sulfoximine, did not produce seizure effects when combined in different proportions.
    Three butyric acid derivatives (GABA, GABOB and S-GABA) did not arrest the actinomycin D seizure effect.
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  • Kuniyuki EGUSA, Shuji SEKI, Takuzo ODA
    1973 Volume 85 Issue 5-6 Pages 183-187
    Published: June 30, 1973
    Released on J-STAGE: March 30, 2009
    JOURNAL FREE ACCESS
    In our previous observation, the T-antigen in unclond mouse embryo cells transformed by SV 40 or SV 40 DNA declined gradually through long term cultivation. To analyze whether this phenomenon is due to selection or reversion and/or conversion, we observed the T-antigen of a series of clones derived from a single cell in the SV 40-transformed cells by the immunofluorescent method.
    The T-antigen was either positive or negative in all cells in each clone, and none of the clones was consisted of the mixture of T-antigen positive and negative cells. The T-antigen negative cells were more predominant in the rate of growth than the T-antigen positive ones in this mouse embryo cells. One reasonable explanation on the data may be that the decline of T-antigen in the uncloned SV 40-transformed cells is due to the selection of the T-antigen negative cells. This fact may be one of the reason for difficulty in establishing the SV 40-transformed mouse embryo cell lines.
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  • Part. I A Improved Method for a Simple Direct Method Counting Absolute Basophils and Eosinophils and its Clinical Significance
    Yoshiro TANIZAKI
    1973 Volume 85 Issue 5-6 Pages 189-197
    Published: June 30, 1973
    Released on J-STAGE: March 30, 2009
    JOURNAL FREE ACCESS
    Improved methods were made to count the absolute numbers of basophils and eosinophils by adding phosphate buffer to Kovács staining fluid. The improved method has following advantages.
    1) Both basophil and eosinophil leucocytes can be easily recognized and identified simultaneously.
    2) The staining fluid is stable for more than one year at room temperature.
    3) Within two hours after filling a pipette, precipittates or ghost cells still do not appear.
    4) This method is simple and convenient for the routine examination like calculation of peripheral leucocytes.
    Changes of basophil leucocytes in the peripheral blood in various diseases were investigated with above described method. Basophilia was shown in allergic rhinitis, bronchial asthma, parasite infection, idiopathic thrombocytopenic purpura, acquired hemolytic anemia, hypothyroidism, iron deficiency anemia, chronic myelogenous leukemia and Addisons disease.
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  • Part. 11. Changes of Basophilic Leucocytes in Bronchial Asthma
    Yoshiro TANIZAKI
    1973 Volume 85 Issue 5-6 Pages 199-210
    Published: June 30, 1973
    Released on J-STAGE: March 30, 2009
    JOURNAL FREE ACCESS
    Using mainly our improved method of Kovács, Changes of basophilic leucocytes in peripheral blood were observed in 181 patients with bronchial asthma and 20 patients with allergic
    occupational asthma caused by inhaling the body fluid of sea spuirts attaching to the shell of cultured Oyster.
    This study shows that basophils have a strict relationship to asthmatic attacks; the basophils show a normal level in non-attack stage as in healthy controls, an increase in pre-attack, and tend to decrease in attack. A close observation on changes in the numbers of peripheral basophils almost make an early detection of the forth-coming attack possible. The threshold of asthmatic attack indicated by basophil count, 65/cmm, is very significant for the observation of forth-coming asthmatic attack, and this threshold of asthmatic attack varied, in some extent, though constant, in each case. These changes of basophils were similar in the cases given corticosteroids. In eosinophils changes were not so clear-cut.
    Concerning a prolonged therapeutic course, basophils in bronchial asthma were over the threshold in the stage of repeated asthmatic attacks, and lower in the non-attack stage.
    Patients with allergic occupational asthma showed these similar changes.
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  • Part. III. Changes of Basophilic Leucocytes in Chronic Myelogenous Leukemia
    Yoshiro TANIZAKI
    1973 Volume 85 Issue 5-6 Pages 211-221
    Published: June 30, 1973
    Released on J-STAGE: March 30, 2009
    JOURNAL FREE ACCESS
    Basophils in peripheral blood and bone marrow in 25 cases with chronic myelogenous leukemia (CML) were counted and morphologically classified by our improved method; basophilis were classified into I to V types. The III and IV types were subclassified to S type with sharply defined granules and diffuse N type. This classification was adapted for basophilograms.
    The basophilogram in healthy controls showed a left type and that in CML generally a right type. It shifted to the left approaching to the normal type in the cases with remission by chemotherapy. In relapsing patients with CML, the basophilogram clearly shifted to the right.
    The basophilogram seen in 2 cases with the pre-stage of blastic crisis of CML indicated a definite right type. It was shown that an increase of S type occurred in the relapsing stage. Therefore, appearance of S type as well as a right type in basophilogram would be a useful clue to follow CML patients clinically. A basophils of S type was considered to be immature. The bone marrow basophilogram was shown to be more shifted to the right than that of peripheral blood, and this tendency became more remarkable in relapse.
    Presence of a right type in basophilogram and appearance of S type are significant to observe the clinical course in CML patients, especially for judging acute exacerbation.
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  • Kuniyuki EGUSA, Keiko SATO, Takuzo ODA
    1973 Volume 85 Issue 5-6 Pages 223-229
    Published: June 30, 1973
    Released on J-STAGE: March 30, 2009
    JOURNAL FREE ACCESS
    Sensitized lymphocytes have been demonstrated to inhibit tumor growth in vivo systems. In SV40-viral oncogenesis, some specified immunization with virns and with virus-induced tumors to the animals results in rejection of further transplantation of the tumor cells. To analyze these phenomena by our specially devised cytotoxicity test, the authors have studied cell mediated immunity for tumor specific transplantation antigen (TSTA) in SV40 viral oncogenesis. The results suggest that sensitized lymphocytes inhibit the growth of heterogenic SV40-transformed target cells as well as syngenic ones. And the lymphocytes from tumor-bearing animals have lower ability of the inhibition on the target cells, which may be explained that the lymphocytes in tumor bearing animals are under the condition of immunologically insufficient state.
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