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Volume 117 (2005)
- Issue 3 Pages 187-
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Volume 71 (1959)
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Volume 70 (1958)
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Volume 65 (1953)
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Volume 64 (1952)
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Volume 117, Issue 3
Displaying 1-7 of 7 articles from this issue
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[in Japanese], [in Japanese], [in Japanese]2005 Volume 117 Issue 3 Pages 187-191
Published: January 04, 2005
Released on J-STAGE: June 03, 2011
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[in Japanese]2005 Volume 117 Issue 3 Pages 193-198
Published: January 04, 2005
Released on J-STAGE: June 03, 2011
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[in Japanese]2005 Volume 117 Issue 3 Pages 199-204
Published: January 04, 2005
Released on J-STAGE: June 03, 2011
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[in Japanese]2005 Volume 117 Issue 3 Pages 205-209
Published: January 04, 2005
Released on J-STAGE: June 03, 2011
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[in Japanese]2005 Volume 117 Issue 3 Pages 211-217
Published: January 04, 2005
Released on J-STAGE: June 03, 2011
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Hikaru Namba2005 Volume 117 Issue 3 Pages 219-224
Published: January 04, 2005
Released on J-STAGE: June 03, 2011
JOURNAL FREE ACCESSA monoclonal antibody (MAb), IK17, was developed and found useful for the analysis of human herpesvirus-7 (HHV7) genome replication. In Western blot analysis, the MAb IK17 specifically detected an HHV7 DNA polymerase subunit, U27protein, expressed in both Escherichia coli (E. Coli) and HHV7-infected cells. Analysis by the same method of truncated U27 proteins expressed in E. Coli demonstrated that the MAb IK17 recognizes an epitope between amino acid1and133of the U27protein. The indirect immunofluorescence (IF) method with MAb IK17detected an unexpected reduction of U27 protein in the infected cells that were treated with a viral DNA polymerase inhibitor, phosphonoacetic acid (PAA), although no reduction of U27 mRNA was observed in the cells by Northern blot analysis.The data suggest the possible usefulness of the MAb IK17 for obtaining clues to characterize an unknown feature of the U27 protein.Immunoprecipitation experiments with the MAb IK17 enabled to detect at least four U27 protein-associated phosphorylated polypeptides in addition to the U27 protein itself. The result indicates the usefulness of the MAb IK17 for qualitative and quantitative analysis of the U27 proteinassociated viral and cellular factors. These analyses contribute to elucidation of the HHV7genome replication system.View full abstractDownload PDF (5501K) -
[in Japanese]2005 Volume 117 Issue 3 Pages 225-234
Published: January 04, 2005
Released on J-STAGE: June 03, 2011
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