Okayama Igakkai Zasshi (Journal of Okayama Medical Association)

The antimitotic effect of cornin extracted from dog intestine

Masao Makiyama

1972 Volume 84 Issue 7-8 Pages 143-155
Published: August 30, 1972
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.84.7-8_143

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The antimitotic effect of cornin extracted from dog intestine, are summarized as follows.
1. Canine intestine cornin (CIC) inhibit the proliferation of HeLa cell in tissue culture. It's antimitotic effect, however, increases depend on the cornin dose per cell.
2. CIC is stable substance. Antimitotic effect of CIC does not decrease after stocking in desiccater over 3 years, and also 2 days preincubation in 37°C. DTT treated CIC show no differences with untreated CIC in growing HeLa cell.
3. Both dialysable and nondialysable fractions show antimitotic effect to HeLa cell. And nondialysable fraction is more effective.
4. Ultrafiltration is done by Diafilter membrane. The most effective fraction of molecular weight is over than 55, 000. The next is molecular weight 20, 00055, 000, the third is molecular weight below 10, 000. The component molecular weight 10, 00020, 000 is slightly effective.
5. The antimitotic effect of molecular weight over than 55, 000 fraction decreases by 2 days treatment in 0.025% trypsin at 37°C.
6. CIC is separated in 3 fractions by Sephadex G200 column. The most effective fraction is FIII. It suggests that the antimitotic action of CIC is more effective in relatively small molecular weight of molecular weight over than 55, 000 fraction.

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Studies on the Structure of Blood Vessels in Malignant Tumors. I

Akira NISHISHITA

1972 Volume 84 Issue 7-8 Pages 157-166
Published: August 30, 1972
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.84.7-8_157

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Studies on the Structure of Blood Vessels in Malignant Tumors. II

Akira NISHISHITA

1972 Volume 84 Issue 7-8 Pages 167-176
Published: August 30, 1972
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.84.7-8_167

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Studies on the Structure of Blood Vessels in Malignant Tumors

Akira NISHISHITA

1972 Volume 84 Issue 7-8 Pages 177-188
Published: August 30, 1972
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.84.7-8_177

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With the purpose of elucidating the role of the stroma for the growth of tumors, the vascular structures in the parenchymal stroma and the surroundings of animal tumors were studied.
In the first chapter, the vascular structures of Bashford tumors and Ehrlich subcutaneous tumors were examined employing the India ink method. The Blood vessels of tumors are derived from their surrounding host connective tissue. The structure of blood vessels of every tumor has features of its own. Further, it was observed that metastatic tumors in the lungs of Ehrlich ascites carcinoma and Brown-Pearce tumors had an individuality in their vascular structures.
In the second chapter, the vascular structures of Ehrlich subcutaneous tumors and first generation transplants of C₃H spontaneous mammary cancers were observed employing ultrasoft X-ray, and the relationship between the blood vessels and the stromaltissue of these tumors was investigated using several special staining methods. Characteristic features were also observed on the vascularization and the stromal tissues of Ehrlich subcutaneous tumors treated with chondroitin sulphate.
In the third chapter, the influences of administration of Bleomycin on C₃H spontaneous mammary cancers were abserved with respect to the vascular struture and stromal tissue. It was found that small tumor vessels were narrowed, obstructed and destroyed by administration of this drug. Further, it was observed that the vasculature and stromal tissue of lungs of mice and humans administered with this agent were likewise damaged, and that prednisolone and chloroquine considerably inhibited these pulmonary damages caused by this drug.

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Study on the Mechanism of phagocytosis

Effect of Ionic strength on Adhesion of Fixed Red Blood Cells to Monocytes

Yoriko SHIMIZU

1972 Volume 84 Issue 7-8 Pages 189-200
Published: August 30, 1972
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.84.7-8_189

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For the purpose to clarify whether electrostatic contributes to the adhesion of red blood cell to monocyte, first phase of phagocytosis, was studied in vitro in ion deficient environments comparing with that in ionic environments. The experiments were carried out by using mouse ascites macrophage and the fresh and fixed red blood cells of mouse and chicken.
Observations revealed that in Hanks' solution, the fixed red blood cells adhered to the monocyte, while fresh red blood cells of both species did not. In isotonic sugar aqueous solutions, however, the fixed red blood cells did not adhere to the monocyte. The test in the varied ionic concentration proved that number percent of monocytes adhering the fixed red blood cells to the total monocytes reduced in proportion to logaritsm of the decrease in ionic strength of the media. Concentration of Ca⁺⁺ and Mg⁺⁺ and pH of the media gave actually no effect on the red blood cell adhesion to the monocyte. The red blood cells fixed and conditioned with methylation treatment adhered to the monocytes even in ion free sucrose solution, but those conditioned with desamination treatment did not.
From these results it is concluded that the ionic strength in the media is a decidive factor for the fixed red blood cell adhesion to the macrophage. Discussion was made on the mechanism of the adhesion from the view point of lyopobic and lyopilic colloid mechanism.

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Relationship between Sulfur Dioxide Concentration in Mizushima Industrial Area

Part 2 [Comparison of Sulfur Dioxide Concentration Measured Practically and Logical Concentration Calculated Using Pasquill's Chart]

Kentaro Inoue

1972 Volume 84 Issue 7-8 Pages 201-207
Published: August 30, 1972
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.84.7-8_201

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The author calculated, using Pasquill's chart, the logical concentration of sulfur dioxide concerning principal 15 chimneys in many places of Mizushima industrial area which had emitted 74 percent sulfur dioxide over Mizushima, and compared concentrations measured practically at the respective station of Fukuda-cho, Mizushima Harbor Office and Amagi with the logical concentration calculated by Pasquill's chart. The results obtained were as follows.
1. The high concentrations of sulfur dioxide measured practically at Fukuda-cho station could be logically explained by the diffusion called C-stability following Pasquill, and those at Amagi station could be also explained by the diffusion called D-stability.
2. Under the condition of each of A and B stabilities following Pasquill, sulfur dioxide diffused itself significantly on C-stability in Pasquill's chart, and in the case of each B and C stabilities, it diffused itself on D-stability in Pasquill's chart.

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[title in Japanese]

1972 Volume 84 Issue 7-8 Pages 209-213
Published: August 30, 1972
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.84.7-8_209

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