Ammonia of the normal rat brain was measured by Conway's microdiffusion method, and amino acid by the paper chromatography, and the following data have been obtained in respective measurements.
1) In the case of the normal rat brain immersed in liquid nitrogen, the percentage of the contents of the following substances proved to be as follows: ammonia, 1.05 mg. per cent; glutamic acid, 140.9 mg. per cent; glutamine, 92.3 mg. per cent; and γ aminobutylic acid, 27.7 mg. per cent.
2) Trepanation and Enuculation of the Brain. Contents of the substances found in the brain frozen solid by dry ice acetone gave the following percentage; ammonia, 0.64 mg. per cent; glutamic acid, 159.9 mg. per cent; glutamine, 94.6 mg. per cent; and γ aminobutylic acid, 37.2 mg. per cent.
3) The amounts of ammonia formed by brain slices were found to be 5.1 μ M/g. for the first one hour, and 7.3 μ M/g. in two hours; and 13.8 μ M/g. in three hours, respectively. In this case, the decrease in the contents of glutamic acid and glutamine during the first two hours paralleled the increase in the contents of ammonia.

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Using 4 strains considered to belong to Staphylococcus aureus and albus, selected from various bacteria isolated from the human mouth as test bacteria and one strain each of the standard-strain bacteria kept in our laboratory as the control, the author made comparative studies of the productivity of hemolysins of both growing bacteria and resting cells as well as investigated effects of various conditions on the production of hemolysins. The results are presented in the following.
1. By passage through the animal the productivity of hemolysin increases markedly in the case of standard strain of aureus while with albus the increase is only slight.
2. On the whole the productivity of hemolysin of the bacteria belonging to aureus strain is greater than those belonging to the albus, and No.2 and standard strain of aureus presents the hemolyzat the highest degree.
3. Nitrogen source is required in the production of hemolysins by resting cells, and the most effective one is pepton; while among amino acids, glutamate, aspartate, alanine and glycine are effective, but there is no amino acid known to be indispensable to the hemolysin production.
Though hemolysin production does not occur when carbon source alone is added, when C-source is added in combination with N-source, the hemolysin production is enhanced. However, in the case of sugars, pH is lowered during the oxidation so there is a tendency to inhibit the hemolysin production.
4. Hemolysin production is inhibited by such metal ions as Fe⁺⁺, Mg⁺⁺, Co⁺⁺ and Cu⁺⁺, and it is likewise inhibited by monoiodoacetate, aureomycine, and chloromycetine.
5. Hemolysin produced by the resting cells are all unstable against heat with exception of standar strain of albus.

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Similar to the previous report, 4 strains belonging to St. aureus and albus selected from the isolated bacteria, and each standard strain of aureus and albus and these bacteria passing through the animal were used as test bacteria. In the present experiment N-source requirement during the growth of these bacteria, and oxidation and production of amino acids by resting cells were studied; and glucose oxidation by growing bacteria and resting cells was compared. The results are as follows.
1. The N-source requirement in the case of albus compared with aureus in general tended to be greater but no significant difference could be recognized between standard strains and those passing through the animal or the isolated strains.
2. With respect to the oxidation and production of amino acids by resting cells, no distinct difference could be found either between standard strains and the bacteria passing through the animal or the isolated strains.
3. On comparing the glucose oxidation by growing bacteria and resting cells of each strain, during the process of glucose metabolism both the strains that passed through the animal and the isolated strains tend to show a weaker oxidation of pyruvate and of those that follow than the oxidation by our laboratory strains.

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The author studied the action of various inhibitors and antibiotics on the glutamaterespiration of staphylococci under the consideration of physiological structure of the cell. Staphylococcus citreus and aureus (Terashima) were used as the test organisms, and L-glutamic acid as the substrate. The results were as follows:
1) Thionine, sodium arsenite and 8-hydroxyquinoline inhibited the respiration of intact cells of staphylococci markedly, but did not inhibit that of cell-free extracts. The inhibitive action of these three sorts of inhibitors was stronger on Staph. aureus (Terashima) than on Staph. citreus.
2) In intact cells, the inhibition of respiration by potassium cyanide and sodium azide was not restored by addition of thionine. In cell-free extracts, however, the inhibition by these two sorts of inhibitors was well restored by thionine.
3) By addition of thionine, the inhibition of the respiration of cell-free extracts by octanole was not so well restored as that by cyanide or azide.
4) 2, 4-Dinitrophenol inhibited the glntamate-respiration of both of the intact cells and cell-free extracts.
5) Of all the antibiotics tested, aureomycin was the only one which noticeably inhibited the glutamate-respiration of staphylococci.

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The author measured the activation energy of the respiration of whole brain homogenate of rats by a Warburg's manometer and obtained the following results. The oxygen absorption of the whole brain homogenate of rats under the experimental conditions was determined following the Arrhenius equation; and the activation energy was found to be about 11, 000 ± 500 cal. However, with what reaction this value might be associated remains still uncertain.

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By studying the effects of NaCN 4 × 10^-4 M on the activation energy of whole brain homogenate of rats, the following results were obtained: namely, the rate of oxygen absorption at the time when NaCN 4 × 10^-4 M is loaded decreases about 14-35 per cent, while the activation energy increases up to about 15, 800 cal. This value can be considered as almost equivalent to 16, 000 cal., and it seems to be due to the cytochrom. cytochrom-oxidase system has turned to the rate determining reaction.

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The author studied the effects of chlorpromazin 1.5 × 10^-4 M on the activation energy of respiration of whole brain homogenate of rats and obtained the results as follows: namely, by loading chlorpromazin 1.5 × 10^-4 M the rate of oxygen absorption decreases about 17-31 per cent, but the activation energy has been found to be about 10, 800 cal. This meàns that the value is the same as in the case without addition of chlorpromazin; and consequently the rate determining reaction is also identical.

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By the inoculation of the antigen prepared from the virus-infected animal organs into the hepatitis patients in Kagato district and the healthy men in other districts, a kind of allergic skin reaction was observed which were specific to a mild or subclinical infection of infectious hepatitis. In order to test the practical value of this skin reaction, the author performed many experiments and obtained the following results:
1) Of all the antigens prepared from various animal organs or tissues, the antigen from liver of the mouse inoculated with Ishihara strain showed the most effective result, and was used in the subsequent experiments.
2) In the forearm, 0.1 cc of the saline solution of antigen was inoculated intracutaneously. The test was tried on 375 hepatitis patients and 125 healthy men, and the frequency curve of the size of flush area was taken to estimate the standard size for the judgement of the result of skin reaction. That over 10 mm in diameter was determined as positive and that under 9.5 mm as negative.
3) The maximum of flush area was reached 20 hours after the inoculation of antigen and lasted till 28 to 32 hours, and it seemed reasonable to decide the result of reaction 24 hours after the inoculation.
4) As the disease became chronic, the specificity and appearance of reaction falled.
5) No noticeable side effect was produced by the inoculation of this antigen.
6) Judged from the frequency curve of the size of flush area, the specificity of the reaction seemed to be higher in the early stage of the disease than in the convalescent stage.

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In the present part, together with the skin reaction which was reported in part, I, the author studied the clinical symptomes, liver function and pathological changes of liverpuncture materials to investigate the interrelation between the skin reaction and these clinical matters. The results were as follows:
1) The skin reaction showed a high positive rate when the hepatitisspecific blood picture, monocytosis, appearance of plasmocytes and relative lymphocytosis, appeared. Inthe prodromal stage, in which various indefinite symptomes developed, a high positive rate of skin reaction of 91 to 96% was obtained. This fact suggested that the skin reaction could be a help to the early and differential diagnosis of infectious hepatitis.
2) Even the cases, in which no noticeable disturbance of liver function was observed, showed a positive skin reaction.
3) In the relapse cases of chronic hepatitis, the skin reaction became severer in the active stage and weaker in the inactive stage, and the non-specific flush became bigger as the disease took a delayed chronic course.

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Authors have had to perform autopsy on a body died after injection of Evipan (Cyclopan) and detected in his brain and blood the crystalline of Evipan by our chemical research.

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By the “roller tube” technic, the author tried the tissue cultivation of the virus of infectious hepatitis. The hepatitis virus isolated by Murakami et al. from hepatitis patients with the embryonated hen's egg or mouse was used throughout this work. The result were as follows:
1) The inoculation of the virus into the medium composed of fragments of skin or muscle of the chick embryo, or suckling mouse did not produce any cytopathogenic effect. The inoculation of each generation of the serial cultivation into the mouse, however, produced the typical pathological changes; this might be taken as the evidence that the multiplication of virus occurred, at least to some extent, in tissue cultures.
2) Judged from the pathological changes in the mouse inoculated with the emulsion of virus infected tissue fragments, the multiplication of virus appeared to be worse in the medium of liver fragments than in that of skin or muscle.
3) When used as the antigen for infection protecting test, the inactivated emulsion of the tissue cultures of virus was less effective than that of the chick embryo by Murakami et al.; this might be the evidence that a high degree of virus multiplication could not be obtained by the tissue cultivation. However, the fact that some degree of infection protective power was obtained by the inoculation of tissue cultures of virus, suggested the possibility of practical application of tissue cultures of hepatitis virus to the vaccination.

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The auther, as a research result of our department intent on the anthropological and esnological investigation to make comparisons between the fingerprint frequency found in those Southern people of which Indonesians of Java consists the center, and that found in Japanese people living in Kyushu and people living in the continent over there to say more properly, having studied in detail the afore-said frequency, in regard to the inhabitants of Koshikijima, which, lying southwest of Kyushu, and against China Continent, Kyushu, or southern island, has a very significant relation anthropologically; as to this, he has investigated as well as compared those results obtained among the inhabitants, dividing them into three group, the east coast and west coast as well as that of Kashima; then, all the results have been considered minutely in contrast to the surrounding tribes, until he arrived at the following somewhat marked purport;
1) Frequency of fingerprints found among those of east-coasters and west-coasters of Koshikijima, and villagers of Kashima; all these groups prove difference in the frequency; easteners and Kashima villagers proved higher frequency in U-type compared to west-coasters, besides, between the former two, the latter proved higher. Whereas, the west-coasters proved higher in W-type frequency, compared to east-coasters or Kashima villagers. Altogether, these group seen to differ racially.
2) The frequency of fingerprints between southerners and northerners of Kyushu and islanders of Koshikijima; east-coaster as well as Kashima villagers, in their privalence in U-type fingerprints, tends to islanders of Tanegashima, or inhabitants of southern Kyushu, i.e., to the local inhabitants of southern Kyushu; while, the west-coasters, in most points, resembles the northern Kyushuist; also, as for south-Kyushu people and Kashima villagers, the latter may be said to resemble more to the southern people, in their prevalence of U-type fingerprints.
3) The frequency of fingerprints found in Koshikijima people, compared to those of various neighbour tribes or races; The east-shore people and Kashima villagers, who are found to be superior in U-type fingerprint, proved to resemble to the Indonesians of Java; while, west-coasters who have proved prevalent in W-type fingerprints, tend to the so-called continentals, i. e., Manchurians, Mongolians (Mongola), Chinese or Koreans (Chosenese). On the other hand, in case we contrast East-coasters with Kashima islanders, the latter, as they prove more frequent in U-type fingerprint, may be justly considered to tend to southern race.

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In order to study some metabolic aspects of Salmonella typhi, the author measured the innert electrode potential of the culture media at 37.5 C with the lapse of time. M/50 phosphate buffer and salt solution were used as the fundamental culture media; glucose, pyruvate, lactate, succinate, malate, glutamate, aspartate and alanine as the substrates; Salmonella typhi 57 S as the test organism. The results were as follows:
1) The most remarkable fall of potential was observed in the media of 10^-3 M glucose, pyruvate and malate, and in 10^-2 M acetate and succinate
2) In the media of lactate or aspartate of the concentration from 10^-1 to 10^-4 M, the potential falled with the decrease of concentration of substrate, while, in that of glutamate, the reverse interrelation was observed. In the media of alanine, no definite interrelation was observed.
3) The lowest potential was below 0 V in the media of glutamate, aspartate and glucose; 0 +100 mV in those of pyruvate, lactate and succinate; +100 +200 mV in those of acetate; +200 +300 mV in that of alanine.
4) When glucose, acetate and glutamate were used as the substrates, the fall of potential was less in phosphate buffer than in salt solution, while, when pyruvate, lactate, succinate, malate, aspartate and alanine were used, the fall of potential was less in salt solution than in phosphate buffer.

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In the present part, the influence of various enzyme-activators and -inhibitors on oxidation-reduction potential was studied. The salt solution (pH 7.2) was used as the fundamental culture medium; Salmonella typhi 57 S as the test organism. The results were as follows:
1) In the medium of glucose or pyruvate Mg⁺⁺ of the concentration over 10^-4M accelerated the fall of potential.
2) 10^-2M KCN inhibited the fall of potential in the media of all the substrates tested, whereas 10^-3M inhibited it in those of the substrates other than pyruvate, succinate, aspartate and alanine, and 10^-4 M inhibited only in that of lactate.
3) NaF of the concentrations over 10^-3 M inhibited the fall of potential in the media of the substrates other than lactate, acetate and succinate. 10^-1 M NaF, however, showed some inhibition even in that of succinate.
4) NaN₃ of all concentrations inhibited the fall of potential in the media of all the substrates other than lactate, acetate and succinate. However, 10^-1M in the medium of lactate, 10^-3 M in that of acetate, 10^-2 M in that of succinate inhibited the fall of potential.
5) Monoiodoacetic acid of the concentrations over 10^-3 M inhibited the fall of potential in the medium of lactate, that over 10^-4 M inhibited it in that of succinate.
6) 2, 4-Dinitrophenol of all the concentrations tested inhibited the fall of potential in the media of all the substrates tested.
7) The inhibitive action of these five sorts of inhibitors to the fall of potential resembled their action to the oxygen consumption of Salmonella typhi.

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In the present part, the author studied the interaction of two substrates to oxidation-reduction potential. Salmonella typhi 57 S was used as the test organism. The results were as follows:
1) The combination of glucose and glutamate, markedly accelerated the fall of potential, while those of glucose and alanine, and glucose and lactate accelerated it moderately.
2) The combinations of aspartate and lactate, glutamate and lactate, malate and glutamate, alanine and malate, and aspartate and alanine did not noticeably accelerate the fall of potential.
3) In the combinations of aspartate and glucose, and lactate and alanine, contrary to the above-mentioned ones, the fall of potential was less than that in each of the substrates.
4) As the cause for the effect of the combinative administration of two substrates, transamination will play a very important role, and the opinion that the hydrogen production by hydrogenlyase is the only cause for the rapid fall of potential, can not be approved as a whole. The essential cause will be, however, disclosed by the studies in future.

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Three strains of R. tsutsugamushi, Karp, Mitani and Tanizawa strains, were inoculated into the peritoneal cavity of guinea-pigs previously injected with chlorpromazine. The peritoneal exudates of thus treated guinea-pigs were diluted with physiological salt solution and were used as the compliment-fixing antigens. The sera of rabbits immunized with living rickettsiae were used as the antisera. The compliment fixation test was carried out in the conventional way. The results are summarized as follows:
1) The peritoneal exudates showed a high compliment-fixing antigenicity, but did not show any anti-compliment-fixing antigenicity.
2) In the presence of homologous and heterologous antisera, the antigen of Karp strain did not show any significant difference in the suppression titer of hemolysis.
3) In both of homologous and heterologous antisera, the antigen of Mitani strain showed strong compliment-fixing action.
4) The antigen of Mitani strain had a compliment-fixing antigenicity, showing no noticeable difference in the suppression titer of hemolysis in both of homologous and heterologous antisera.
Consequently, the peritoneal exudates of guinea-pigs treated as mentioned above, in regard to compliment-fixing antigenicity, have the same rickettsial specificity, but no noticeable strain specificity.

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In a previous part, it was reported that the peritoneal exudate obtained by intraperitoneal inoculation of R. tsutsugamushi into guine-pigs previously injected with chlorpromazine was excellent antigen for the compliment fixation test. In the present part, the author investigate d the specificity of this compliment-fixing antigen to Tsutsugamushi disease. The results are summarized as follows:
1) In the presence of the sera of fleck fever, typhoid fever, leprosy, tuberculosis patients and pregnant women, the antigen of peritoneal exudate of R. tsutsugamushi did not show any significant suppresion of hemolysis.
2) In the sera of Tsutsugamushi disease patients, the antigen of every strain of R. tsutsugamushi, even highly diluted one, showed the suppresion of hemolysis.
In short, the compliment fixation test with the antigens of Karp, Mitani and Tanizawa strains was quite specific to Tsutsugamushi disease and did not show any group reaction; this fact suggests that this compliment fixation test is of great use for the diagnosis of Tsutsugamushi disease.

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It is said that Neill-Mooser reaction in the scrotum of the male guinea-pig is specific to R. mooseri, but R. prowazeki also sometimes causes the non-specific reaction, so-called false Neil-Mooser reaction. The author studied on the substance of Neill-Mooser reaction by investigation of the figures of cells in the exudate of the tunica vaginalis of scrotum. The results are summarized as follows:
1) In every case, the tunica reaction by R. mooseri was markedly positive and lasted for a long time. The reaction by R. prowazeki was accidental and not specific.
2) Even inthe cases of inoculation of R. mooseri into male guineapigs, the reaction did not always appear typically in those weighing under 400 g.
3) In the positive phase of the scrotal reaction in guinea-pigs inoculated with R. mooseri, activation of fibrohistiocyte system appeared in the tunica vaginalis, and the great majority of rickettsiae became visible in the serous cells.
In short, the affinity of R. mooseri for the tunica vaginalis of the guinea-pig was specific, while that of R. prowazeki was not specific.

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