Okayama Igakkai Zasshi (Journal of Okayama Medical Association)
Online ISSN : 1882-4528
Print ISSN : 0030-1558
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Volume 79, Issue 9-10
Displaying 1-13 of 13 articles from this issue
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  • Three Cases what Appeared to be “Porcelain Gallbladder”
    Kaname AONO, Masatada TANABE, Shinya NOMA, Yasumasa KUMADA, Yoshimasa ...
    1967 Volume 79 Issue 9-10 Pages 727-731
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_727
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    Present paper describes the findngs of three cases what appeared to be the case of “Porcelain gallbladder” showing the X-ray picture similar to gall stones in the resected bladder and Calcium deposite on the bladder wall.
    It was in 1926 that “Porcelain gallbladder” case was first reported by Flörcken. Since then there appeared reports of such cases by Yamamoto, Niizuma and others, but this is a relatively rare diseases. It is sometimes accompanied by gall stones and in other cases without any stones.
    In the diagnosis of this disease by X-ray it is possible to identify this case by the roentgenograms of affected field and also by tomography of that region.
    We are able to examine three cases that we encountered by simple X-ray pictures, X-ray pictures with contrast medium as well as by tomographic pictures of the affected region.
    Some discussion was made comparing the findings of our cases with those available in literature.
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  • Antimitotic Action of “CORNIN” Extracted from Rabbit and Dog Organ
    Toshiaki TERASAKA
    1967 Volume 79 Issue 9-10 Pages 733-742
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_733
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    It is generally observed that there are various organ-specific mitotic rates in regeneration and compensatory growth. Nevertheless, each tissue can proliferate well in a suitable culture medium.
    These facts suggest that there are regulating substances for cell division.
    The author extracted an antimitotic substance “CORNIN” from rabbit and dog organs by the alcoholic fractionation, mainly from muscle layer and mucous layer of digestive organs.
    Each cornin of internal organs has less antimitotic effeet on sea urchin eggs than that of muscle or cornea. Furthermore, there are no difference between muscle layer and mucous layer as regards the antimitotic effect. There is no difference in the qualitative analysis of cornins for protein and sugar. Typical protein waves are shown by polarogram excepting cornin of regenerating rat liver. The ultra-violet absorption curves show the same maximum absorption peak at 260 mμ, which is the same as that of corneal cornin, but is different from muscle cornin that shows the peak at 249 mμ. The paper electrophoresis does not show any remarkable fifferences among these cornins.
    With respect to the cornin activity, there can be seen no remarkable difference between muscle layer and mucous layer.
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  • Studies on Intracellular Crystals
    I. New Crystals Arising in Bone Marrow Cells of Various Animals
    Hiroshi MATSUMORI
    1967 Volume 79 Issue 9-10 Pages 743-750
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_743
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    Hitherto unknown intracellular crystallizations were discovered in neutrophils and megakaryocytes of various animals.
    Animals used were normal C57 and Cb mice, normal hybrid mice. these mice with leukemoid reaction induced by Saponin injection, C58 mice with myelogenous leukemia (OHS ML, No. 1), C58 mice with lymphocytic leukemia (OHS-LL, No. 1), normal rats, guinea-pigs and dogs.
    Cells of bone marrow or spleen of these animals were observed by means of tissue culture or supravital preparations. A few hours after the preparations were ma e, shape, nee e shaped, or pindle-shaped, strongly refractile crystals were observed arising in neutrophils and later in megakaryocytes. Those crystals were positive in birefringence and do not seems to be of extracellular origin.
    The crystals I found have not been reported in the literature.
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  • Studies on Amino Acid and Protein Metabolism of the Brain with the Use of [U-14C]-Glucose
    1. Protein Metabolism of Normal Cat Brain and the Brain Under Chronic Compression by Infusion Method
    Buntaro OMORI
    1967 Volume 79 Issue 9-10 Pages 751-762
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_751
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    There are many reports on the protein metabolism of the brain but it seems that much remains to be clarified as regards the structural chemistry of the brain proteins. For the purpose to elucidate this problem, the author studied histological changes in the cat brain at the time when the frontal lobe was placed under chronic compression by the extradural compression method of Ishii et al., and simultaneously studied the incorporation of 14C into each subcellular unit protein of the brain tissue after intravenous infusion of [U-14C]-glucose. Further, the incorporation of 14C into what Otsuki et al. call acid-ethanol soluble proteins and residual proteins was investigated. Brain proteins were fractionated by DEAE Sephadex column chromatography as well as by the disc-electrophoresis with polyacrylamide gel, and compared these fractions with these of normal cat brain. The results of the study are briefly summarized as follows.
    High molecular substances of the mitochondrial fractions of the cat brain under the chronic compression showed a higher relative specific activity (RSA) to the blood glucose than those of the normal cat brain. In the case of acid-ethanol soluble proteins in the brain under chronic compression, just as in the case of normal cat brain, particulate fractions, especially crude mitochondrial fractions, revealed a higher incorporation of 14C, and also their RSA was higher than that in normal brain.
    In the fractionation by DEAE-Sephadex column chromatography, the mitochondrial fractions of the brain under chronic compression showed a specific peak of the eluate that dissolved with 0.8 M NaCl. This substance was the protein fraction that showed the mobility identical with prealbumin fraction obtained by the dis-electrophoresis with polyacrylamide gel.
    Looking at the 14C incorporation by each protein fraction separated on DEAE-Sephadex column, the radioactivity was observable in the portion dissolved only by 0.02 M sodium phosphate buffer.
    This substance, when subjected to the disc-electrophoresis with polyacrylamide gel, showed the mobility identical with γ-globulin fraction.
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  • Studies on Amino Acid and Protein Metabolism of the Brain with the Use of [U-14C]-Glucose 2
    A Study on the Amino Acid Metabolism in Normal Cat Brain and the Brain Under Subacute Compression of the Cats Under Nembutal Anesthesia
    Buntaro OMORI
    1967 Volume 79 Issue 9-10 Pages 763-770
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_763
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    In the previous paper dealing with the protein metabolism as well as the structural chemical aspects of the brain proteins in normal cat brain and the brain under chronic compression by means of infusion method, it was reported that organic changes of the brain affect the protein metabolism.
    This communication briefly describes the results of the experiment conducted by the infusion method on the amino acid metabolism studied with the use of [U-14C]-glucose while comparing with the same metabolism in normal cat brain. Further the effect of the functional changes mainly induced by Nembutal on the glucose metabolism in the brain was studied by means of brain perfusion. and this effect was compared with the effect of Nembutal anesthesia as studied by infusion method. The study was also focused on the metabolism of glutamic acid and aspartic acid which are the protein components of crude mitochondrial fractions in the brains of those placed under subacute compression and normal cat brain. After the infusion of [U-14C]-glucose, the incorporation of 14C to free glutamic acid and aspartic acid of the brain was higher in the brain under subacute compression than in normal brain, i. e. the relative specific activity (RSA) was higher in the former than in normal brain. This tendency was more marked in aspartic acid.
    In observing the glucose metabolism by injecting Nembutal into carotid artery during the brain parfusion, the metabolic pattern at this stage showed a pattern intermediate between that during the high functional state and the low functional state of the brain under perfusion. This resembles the metabolic rates of glutamic acid and aspartic acid of the normal cat brain under a slight anesthesia by the infusion method. Only in the case of aspartic acid the relative specific acitivity (RSA) in the brain during the infusion was considerably lower than RSA during brain perfusion under the influence of Nembutal.
    In the protein component amino acid metabolism of crude mitochondrial fractions. RSA of the protein component glutamic acid was higher under subacute compression state than RSA of normal brain.
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  • Eosinophilic Gastritis Report of two Cases
    Michio YAMAMOTO, Sôichi NISHISHITA, Osamu HIRAMATU, Srôsuk ...
    1967 Volume 79 Issue 9-10 Pages 771-777
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_771
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    Recently, as we have encountered two cases of eosinophilic gastritis, we have described about these X-ray findings etc.. And attention is called to the feasibility of clinical and roentgenographic diagnosis.
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  • Re-examination of the Punch Method for Anti-inflammatory Assay with Practical Applications
    Akio MASADA
    1967 Volume 79 Issue 9-10 Pages 779-789
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_779
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    Ungar, Kobrin and Sezesny (1959) devised an anti-inflammatory assay method the principle of which is to punch out the skin pieces from the inflamed and control sites and weigh them to obtain the net weight increase of the skin produced by phlogogenic substances. The present author carefully re-examined this method with special consideration on the experimental conditions and the reproducibility of the results. The inflammatory edema was induced in animals by the intracutaneous injection of anti-rat rabbit serum, serotonin, bradykinin, dextran, carrageenin, formalin (in rats) and histamine (in guinea pigs).
    It was found that the weight increase (edema intensity) of the skin induced by phlogogenic substances is related with the body weight of animals, the caliber of the punch and the pH of the vehicle in which irritants are dissolved. Satisfactory results were obtained with the use of male rats weighing 150±10g, the punch of 12mm caliber, and 0.9% physiological saline adjusted to pH 7.2 as the vehicle. As the skin could be punched out from 4 test sites and 4 control sites of one animal, it was possible to estimate the edema intensity with good reproducibility of the results using a relatively small number of animals. There was found a linear correlation between log doses of the phlogogenic substance and the weight increase of skin. A linear correlation was also obtained between the log doses of the anti-inflammatory agents tested and the percentage inhibition of weight increase of inflamed skin.
    With respect to anti-rat rabbit serum edema, the anti-inflammatory effect of oxyphenbutazone was somewhat longer lasting than that of phenylbutazone. Peroral phenylbutazone was slightly more effective than intramuscular. These results reflect well the course of blood level of these agents reported in rats as well as in man. The author depicted log dose-effect curves of 7 kinds of glucocorticoids and of 12 non-steroid anti-inflammatory agents of current clinical use. The order of the anti-inflammatory potency of these compounds coincided with the order of the clinical effect surmised on the basis of doses. Phenylbutazone, oxyphenbutazone, hydrocortizone, prednisolone, dexamethazone, chloroquine, aminopyrine and aspirin also showed clear inhibitory effects on the inflammatory edema induced by histamine, serotonin, bradykinin, and dextran. Discussion was made on the simplified experimental design with this method, for the anti-inflammatory screening of newly synthesized compounds, with some concrete examples.
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  • Inhibitory Effect of Adrenal Glucocorticoids on Histamine Release by Sinomenine
    Hiromu KANAI
    1967 Volume 79 Issue 9-10 Pages 791-796
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_791
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    Hydrocortisone, prednisolone and dexamethasone clearly inhibited the reduction of skin histamine of albino rat with their optimal doses, individually not affecting the histamine content, when injected intramuscularly in divided doses 24 hours and 1 hour prior to an intraperitoneal injection of sinomenine hydrochloride 50 mg/kg. Their inhibitory effect reached the maximum, approximately 50 per cent inhibition, with the doses: 10 mg/kg of hydrocortisone acetate, 5 mg/kg of prednisolone acetate and 1 mg/kg of dexamethasone acetate respectively, whereas at the dosages larger than the above mentioned the inhibitory effect rather decreased. Relative potency of these glucocorticoids, in terms of doses producing the maximum inhibitory effect, was proportional to their anti-inflammatory effects. These results are reconfirmation of the earlier findings reported by Yamasaki and Yamamoto (Jap. J. Pharmacol. 13: 223, 1963), on the inhibitory effect of glucocorticoids on histamine release induced by dextran.
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  • Cytological Studies on Cancer Cells
    Part I. Cytological Studies on the Free Cell of Mouse Ascitess Hepatoma 134
    Seihei MIYAKE
    1967 Volume 79 Issue 9-10 Pages 797-804
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_797
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    The present study was designed to determine cellular charactors of the carcinoma of the human utrine cervix utilizing Microspectorophotometry for nuclear DNA and Squash technique for chromosome.
    A fundamental study was carried out analysing chromosome number, nuclear DNA and nuclear volume in free cell of mouse ascites hepatoma 134.
    Results were as follows:
    1) Since the highest peak of chromosome number was observed at hyperdiploid range (44). it was considered as the chromoseme number the stem cell. Another small peak, however, was also noted at triploid range.
    2) A amount of nuclear DNA in hepatoma cell was found to be higher than in normal lymphocyte and was distributed in wide range.
    3) A significant correlation was observed between chromosome number and amount of nuclear DNA.
    4) Correlations were also noted to amount of nuclear DNA to nuclear volume, cell protein to cell volume and amount of nuclear DNA to cell protein respectively.
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  • Cytological Studies on Cancer Cells
    Part II. Cytological Studies on the Cancer Cell of the Human Uterine Cervix (With Special Reference to the Chromosome and the Amount of Nuclear DNA)
    Seihei MIYAKE
    1967 Volume 79 Issue 9-10 Pages 805-820
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_805
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    Chromosome number, amount of nuclear DNA, and nuclear volume were measured in 14 cases of the carcinoma of the cervix of the human uterus in which chromosome were clearly demonstrated out of 120 cases.
    Following results were obtained on correlations in parameters measured and vaginal smear to the appearrance of chromosome demonstrated by Squash Method.
    1) Chromosome number of the cancer cell of the uterine cervix exhibited a wide range of number variations from 35 to 136. The mode of chromosome number was observed in all cases. The highest peak of the frequency was noted at diploid range, particularly at hyperdiploid range. Another peak were noted at triploid and tetraploid ranges. It was decreased in sequence of triploid and tetraploid range, respectively.
    Number of cases showing one mode was much higher than these having two modes.
    2) While wide variation was noted in shape and size of chromosome of the cancer cell when compared to the normal in Karyotype analysis, no definit tendency was obtained in the cancer cell.
    3) Amount of nuclear DNA in the cervical cancer cell was found to be higher than in normal cell and was distributed in wide range. Same tendencies were observed in frequency and type of mode of nuclear DNA as chromosome number. Amount of nuclear DNA was correlated to chromosome number.
    4) Nuclear volume of the cervical cancer cell showed a wide variation, however same size as normal cell was also demonstrated. Nuclear volume was correlated to amount of nuclear DNA.
    5) Chromosome number and nuclear DNA did not correlate to patient age, clinicalstage nor pathologic findings.
    6) High apperance rate of chromosome by Spuash technique was obtained in the exopbytic type of cervical cancer in which appearance of malignant cell in vaginal smear was also high.
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  • Studies on Vectorcardiogram
    Part I Analysis of the T Loops in Young Healthy Japanese
    Masanobu IMAI
    1967 Volume 79 Issue 9-10 Pages 821-828
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_821
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    Analysis of the T loops of the vectorcardiogram (Frank's lead) in young healthy Japanese and its changes with aging and sex distinction were done. Results were as follows.
    1) The magnitude of the QRS and T maximum vector was the greatest in the first decade in the male and in the second decade in the female.
    2) T magnitude/QRS magnitude Ratio was greater in the male than in the female.
    3) L/W ratio was smallest in the first decade. That is the T loop in first decade was most circular.
    4) The polar vector of the T loop was located in the second quadrant, and the greatest in the first decade.
    5) The T loop was large and rounded ellipse ln shape. On the other hand, the T loop found in coronary sclerosis and the hypertensive was small and circular.
    6) It is postulated that the change of shape of the T loop was influenced with the vector of developing posterior wall of the left veutricle.
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  • Studies on Vectorcardiogram
    Part II Analysis of the T loop in the Aged
    Masanobu IMAI
    1967 Volume 79 Issue 9-10 Pages 829-839
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_829
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    Subjects with normal standard 14 leads EGG were chosen. Relationship of the T loop with blood pressure, serum cholesterol, funduscopic findings, (especialy arteriosclerotic changes) age and sex distriction was studied. Results were as follows.
    1) The T loop tended to be small, and circular, and to be located superiorly with the aging in both sex.
    2) Location of the polar vector of the T loop tend to convert into the first quadrant froom the second one.
    3) The shape of the T loop was not correlated with blood pressure, serum cholesterol and fundoscopic findings and it was more related to the aging. The T loop was large and circular in the forth decade.
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  • [title in Japanese]
    1967 Volume 79 Issue 9-10 Pages 841-845
    Published: October 30, 1967
    Released on J-STAGE: March 30, 2009
    DOIhttps://doi.org/10.4044/joma1947.79.9-10_841
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