With transparent specimens (Spalteholz's method) injected with dyes and tissue sections, the author studied the developmental course of medullary vessels in the scapula of 60 human embryos for the periods covering the first embryonal stage to the terminal stage, and obtained the following results.
1) The primary bone marrow in scapula starts its formation in the latter part of the third embryonal month (twelfth gravid week) with the primary medullary primordium which penetrates into three sites in the neck of scapula.
2) Nutrition arteries and veins (A. and V. nutricia) start to develop along with the enlargement of the primary medullary spaces and are distributed beginning at the neck of scapula radially and extensively, and veins, with no distinction of trunk and capillary, possess the vascular diameter far larger than that of arteries, presenting a picture of intra-tissue spaces.
3) By the fouth embryonal month, enchondral ossification starts, and along with the progress of bone formation the central part of scapula is almost completely occupied by bony substance. In the latter part of the fourth embryonal month, the blood vessel system is divided into two parts having the base of spina scapulae as the center, at the same time auxiliary nutrition vessels begin to penetrate into spongy substance of residual Margo vertebralis, thus offering an aid to the blood circulation.
4) No marked changes with the progress of gravid month can be observed in the mode of distribution of medullary vessels later than the fifth embryonal month. The nutrition artery enters into scapula from the base of spina scapulae and divides itself into two branches. One of the branches runs along the lower edge of the base of spinsa capulae towards Margo vertebralis while the other descends along Margo axillaris and on its way anastomoses with nutrition arteries coming from one or two other nutrition canals, and finally sends out minute capillaries, elongating extensively cross-wise along Margo vertebralis; and these capillaries surround the central part of bony substance. Now the trunk and peripheral vessels can be distinguished in nutrition veins.
5) The minimum of two, the maximum of ten and usually 4-6 of nutrition canals have been recognized, and a slight tendency of an increase in number of these canals is observable along with the progress of growth.
The largest and most commonly found nutrition canal is located at the base of spina scapulae. and when other canals do exist, they are generally located at a fixed location.
6) In the beginning of the fourth embryonal month, cartilaginous canals appear near the root of coracoidal process in the cartilarginous part of Fossa articularis, and assuming a peculiar mode of development, many of these canals are seen penetrating into the vicinity of the cartilaginous part of Fossa articularis and into the coracoidal process; and in the seventh embryonal month one of these canals is communicated with the medullary vessel of Fossa articularis.

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With the aid of transparent specimens (Spalteholz's method) injected with dye and tissue sections, the author investigated the developmental course of medullary vessels in the ilium of 60 human embryos for the periods ranging from the first to the terminal embryonal stages, and obtained the following results.
1) The developmental courses of both the primary bone marrow and the meduallry arteries and veins in the ilium do not differ to any great extent from those observed in the scapula, and the periods as well processes of development are almost the same
2) In the stages later than the fifth embryonal month, no marked changes with the progress of gravid months can be observed in the mode of the distribution of medullary vessels. All the arteries, penetrating more than two at a time, are anastomosed with one another and with many anastomoses of capillaries thus forming networks, they are densely distributed. Veins can now be distinguished into trunks and capillaries.
3) The minimum of two, the maximum of seven, and usually 3-5 nutrition canals can be recognized; and under all circumstances the largest nutrition canal is situated in front of Facies auricularis of the inner side of ilium. In case there are other nutrition canals, they are generally located in a fixed position.
All nutrition canals run towards the base of Ala ossio ilium, which is the center of bone marrow, and pass through the bony substance obliquely.

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Using transparent specimens (Spalteholz's method) injected with dyes and tissue slices, the author observed the distribution of medullary vessels in the pelvis, ischium and pubis in particular, of 60 human embryos covering the periods from the first the terminal embryonal stages, and obtained the following findings.
1) Ossification commences in the beginning of the fourth embryonal month for ischium while the ossification of pubis, though individual differences are marked, begins in the period between the beginning and the middle of the fifth embryonal month.
2) Main nutrition canals of both ischium and pubis can be observed in respective inner side of Pars acetabularis. Principal nutrition arteries and veins both in ischium and pubis form dense networks with numerous anastomoses of respective capillaries and are distributed extensively.
3) Besides these principal nutrition arteries and veins, 1-3 auxiliary nutrition vessels can be observed entering into the bone marrow.
4) Cartilaginous canals of the pelvis enter in the beginning of the fourth gravid month, and cartilaginous canals, one each, are seen entering into Fossa acetabuli and into the exterior side of Pars acetabularis of ischium. Moreover, with progress of gravid months many cartilaginous canals can be observed entering here and there, anastomosing with each other or communicating with respective medullary vessels.

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The author. intending to carry out some Penicillin anaphylaxis experiment in view to the individual phenomenon, has immuned certain rabbits by dint of cow's serum for antibody source, and then has divided them in two groups owing to their degree of antibody production (strong or weak); next, by sensitizing some penicillin on both, has taken observations on the same phenomenon, making such as the reticulo-endotherial function, serum protein fraction, blood picture, local hypersensitivity as well as penicillin-sensitized erythrocyte agglutination reaction for indexes; results were as follows:
If one makes the precipitin production as an index in any antibody production, it is usuall assumed that there exists groups of rabbits proving either strong or weak in antibody production; therefore, in case one takes a survey on (penicillin) local-sensitivity as well as the penicillin anaphylaxis in view to certain agglutination reaction of penicillin-sensitized erythrocytes, not to say of other proofs such as reticul-oendotherial function, serum protein fraction, changes of blood picture, or the local hypersensitivity, it may roughly be surmised that certain penicillin anaphylaxis might be induced in the group strong in antibody production, from which I have concluded that some individual phenomenon interferes with any penicillin anaphylaxis.

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The author, by sensitizing penicillin on the certain rabbits, that have been decreased in antibody production by splenectomy, indiaink blockade and carbon tetrachloride injection, has proved by experiment Congored Index, electrophoretic fraction of serum protein, blood picture, penicillin-sensitized erythrocyte agglutination reaction and local hypersensitivity. As a result, the author has found the decrease of sensitivity for penicillin of the rabbits.

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In 1957, Yabe reported that the combined administration of glutamate and glucose markedly accelerated the respiration of Micrococcus pyogenes var. aureus (Terashima) over the sum of those in each of the two substrates, and that this phenomenon was due to the important role of glutamate as a sole sparker or entering site of the citric acid cycle of M. pyogenes var. aureus (Terashima). In the present experiments, the authors investigated this phenomenon in regard to pH.
In the presence of glutamate and glucose, oxygen consumption or carbon dioxide evolution was nearly the same at all pHs tested, whereas ammonia formation was the best at pH 6.2, which was the optimum for glutamate oxidation by M. pyogenes var. aureus (Terashima); this result seems to sustain the conclusion given by Yabe.
For all of glutamate, aspartate and alanine, the oxidation optimum pHs were lower in M. pyogenes var. aureus (Terashima) than in pyogenes var. albus and citreus.
From thsee results, it is easily inferred that the cytoplasmic membrane of M. pyogenes var. aureus (Terashima) is a pretty peculiar one compared with those of the other two micrococci, and that these peculiar physiological properties of the cytoplasmic membrane have something to do with the previously reported peculiar aspect of the citric acid cycle of M. pyogenes var. aureus (Terashima).

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Authors have discribed the case that had recently encountered and dissected a newly-born child afflicted with a combination of Encephalocele, Anophthalmos, Polydactylism, masculine Hermaphrodism and etc.

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The author, employing certain rabbits, and after having induced in them a bone fracture experimentally, examined for 9 weeks since that date, such facts as changes that have occurred to serum protein, function of reticulo-endotherial system, number of leucocytes, erythrocytes, Hb, Ht, or B. S. G.: and further, by examining the effects that have ensued the administration of Platonin, finally has obtained results as follows: Those changes that have occurred to the number of leucocytes, erythrocytes, Hb, Ht, or B. S. G., have proved at a gla ce to be slight, yet showed strong in preaction in proportion to the seriousness of the disease, which, however, recovered by the administration of Platonin. The function of the reticulo-endotherial system has proved to lower at the beginning of injury, but soon revived, even where, the lowering degree has been slighter and sooner recovered in slighter affections, rather than in more serious cases. Moreover, even this thenomenon has been mitigated by the administration of Platonin. As to serum protein, due to the changes in each fraction, certain significance has been attached to A/G value; in changes of which too, the decrease at the beginning of injury showed to be slight in slighter diseases; and besides, proved to recover sooner. Moreover, this tendency has been strengthened by Platonin.

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The auther has attempt to study the propagating mechanism of the excitement, which can be thought as the origin of the epileptic convulsion. The action potential has been recorded from cerebral cortex, basal ganglia, nucleus of midbrain and muscles of extremities during convulsion, which precipitated by intravenous administration of metrazol (10% solution of Cardiazol) on cats. Needle electrodes (200 enamel insulated steel wire) were used as recording electrodes, and the spike wave (duration 20 to 60 msec. amplitude 50 to 100 μV by abovementioned electrode) was applicated as an indication of the excitement.
1) Motor cortex, caudate and pallidum, thalamus and black substance have demonstrated their important function in the propagating mechanism of the excitement. And motor cortex has been more sensitive than other structures.
2) On the occasion of metrazol convulsion, the spike wave is detected in the first place at cerebral cortex. The spiking at cerebral cortex propagates to basal ganglia with some seconds delay time, and then the spiking propagate also to basal ganglia. Moreover, the spiking at basal ganglia propagate to mid-brain nucleus. The onset of a convulsive movement is earlier than the beginning of the spiking at basal ganglia, and is delayed from the beginning of the spiking at cerebral cortex. The convulsive pattern of muscles change from former clonic convulsion to tonic convulsion in the course of propagation of the spiking to basal ganglia.
3) The auther has presumed that a typical epileptic convulsion, which has clonic-, tonic- and clonic stadium a its components, should have above mentioned propagating mechanism of the excitemnent from electroencephalographical findings.

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In the present paper, efforts have been attempted to analyze alteration of the basal metabolism before and after gynecologic operation and to contribute to the treatment.
The studies were made at the Dept. of Obstetrics and gymecology, Okayama University Medical School, on 77 cases of laparotomy. The basal metabolism was measured by Knipping's apparatus before and after the operation and the value obtained was referred to Harris-Benedict's table to determine the percentage of the basal metabolism. The summary of the results is as follows:
1. Normal value was discussed in the evaluation of 26 healthy nonpregnant woman who had not been suffered from any serious diseases. It was 11.27% 13.16 m 9.38 21.07 x₀ 1.47
2. In 77 cases before operation, it was 17.41% on the average and was significantly increased compared with the normal value.
3. In the analysis of these values, the basal metabolism of cases with fibroma uteri and carcimoma of the cervix was significantly increased compared with those of the healthy women. On the other hand, those of the cases with benign ovarian tumors had no significant increase.
4. Each value which was measured on the 5 th, 10 th and 15 th day after operation, had a close relation with that before operation. That is, in cases which had high values before operation, basal metabolism dropped after operation and in cases with low values, it was accelerated after operation. These tendency was observed in all cases.
5. As to analysis of values of each disease, cases with fibroma uteri was accelerated significantly on the 10 th day after operation as compared with those before operation and of the 5th day after operation. In cases with benign ovarian tumors no significant difference was obtained between values after and before operation. Measurements of patients with carcinoma of the cervix were taken on the 5 th, 10 th, 15 th, and 20 th after operation and there was no significant difference each other between these values. But the average value which was measured on discharge was significantly lower than those before operation and of the 20 th day after operation.
6. As to analysis of values at the time of their discharge, a significant difference was recognized only in a case with fibroma uteri and it was significantly lower than that before operation inspite of acceleration compared with the normal value.

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The author estimated by Warburg's manometer, the tissue metabolism of normal (10 cases) and carcinomatus (73 cases) patients who were hospitalized in Okayama University Hospital. The influence of Roentgenrays on cancer tissue was also studied after irradiating (900r, 1800r 2700 r and 3600 r each) on them, and the relation between its clinical or histological findings and cancer tissue metabolism rate or metabolic X-ray sensitivity.
Following results were obtained:
1) Tissue metabolism of the normal cervix is relatively high in the respiration rate, but low in the glycolytic action.
2) Tissue metabolism of non-treated uterine cancer is slightly higher in the respiration rate and notably higher in the glycolysis, comparing to those of the normal uterine tissue.
3) Influence of X-rays on the cancer tissue metabolism: the respiration rate is markedly suppressed and becomes lower than that of the normal tissue by the irradiation described above, and the glycolytic action is not suppressed by the irradiation of 900 r, but is suppres sed, not less than the rate of the normal tissue, by irradiation of over 1800 r.
4) Relation between the non-treated cancer tissue metabolisn and its clinial and histological findigs. The metabolism rates increase in following cases; advanced cases classified clinically, I type in Nagase's classification, cases after menopause and large -tumor-bearing group.
5) Relation between the metabolic X-ray sensitivity and the clinical and histological findings: In creases of X-ray sensitivity are observed in following cases; younger age group, IV-VI type in Mandelstamm's method, cases schowing intersive changes in the tissue and increasing T. P. T-positive cells, and spinous-cell group in Martzloff's classification.
6) Metabolism suppressing rate, i.e. metabolic X-ray sensitivity is closely related to the histomorphological findings, and is thought to be one of the important methods to determine the X-ray sensitivity.

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The author has performed polarographic cancer tests, first reaction (abb. F.) by the Brdicka's original method, filtrate reaction (abb. D.) by the Müller's method. The positive percentages were; F. 90.9% and D. 81.8% in the gastric carcinoma, both 82.3% in the whole carcinomas. Although each reaction was not specific for cancer, it was aimed to improve the sensitivity, after choosing the distinguished one out of the non-specific: The positive percenteges were 90.9% in the gastric cancer and 88.2% in the whole carcinomas, when they were calculated from the formula F./2D.×15. And the positive percentage revised in accordance with the protein contents was 87.1%.
F. in polarographic cancer test, comparing to the others, corresponds to Kürten, Hichijo Black's tests, which determine SH-activate grade. Huggins' and Jensen's reactions revised with the protein contents show similar rate to the Tropp's.

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a) The rabbits under those conditions such as, non-treated, R. E. S. stimulated, R. E. S. blocked, splenectomized, liver protected, and cachectic, were given human cancer extract intravenously for seven days, determining the protein waves of the blood serum. It was found that the wave height of F. decreased and of D. increased, A/G and α and γ globulins increased, when the cancer extract was injected into them. In the liver protected and in the R. E. S. blocked cases the decreasing rate of F. wave height was less, and in the liver protected and in the splenectomized cases the increasing rate of D. wave height was also less.
b) The filtrate protein waves were determined polarographically in the saline extract of the gastric cancer tissue, the mucosae of gastric cancer and of gastric ulcer. In the cancer extract the fluctuation in wave height did not appear even after alkali denaturation.
c) In the paper-chromatograph completely similar nine spots were observed in the cancer tissue, the mucosae of gastric cancer and the ulcer.
d) Filtrate protein wave was determined, after keeping the cancer tissue extract and the serum at various temperatures for 30 minutes and in the several concentrations mixed in vitro, and was compared with the result obtained in the non-mixed after the same procedure. In the temperature of 20°C and the protein concentration of 1.1 gm. %, the mixed one showed higher wave than the non-mixed. As a control, same experiment was performed in the extract of mucosa of gastric ulcer, but it showed no differences between the mixed and the non-mixed.
In short, the cancer extract which elevated the protein wave of rabbti serum filtrate is more denaturated than the extract of normal gastric mucosa, and the cancer extract has a direct action to the serum protein and to elevate the filtrate protein wave even in vitro.

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The author has determined the polarographic protein wave of the gastric juice taken fractionally after histamin injection. In F. the polarogram was taken in 1/200 sensitivity, after adding 3 cc. of 1/20 N. HCL into 2 cc. of the gastric juice, and 10 cc. of Co⁺⁺ test solution into 0 2 cc. of the former mixture 15 minutes later. Bordering 60 mm of wave height the gastric carcinoma showed a high wave and the ulcer a low wave.
D. was performed by the method of Wada and the wave height was compared between the contents before and after 20 minutes of histamin injection, its percentage was 90%.
Polarographic protein wave was determined in the supernatant and in the deposit after centrifuging the solution (fractionated gastric juice 1 cc. +acetone 1.5 cc.) kept at 40°C for an hour (used Co⁺⁺⁺ test solution). In the supernatant same as in the acetone deposit a protein wave producing substance was found, and the protein wave curve showed similar rise and fall tendencies to the filtrate protein wave curve.

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I have studied the Thorn's test in lungtuberculosis by the administration of Adrenalin. The results are as follows:
(1) The eosinophil diminition ratc (E. D. R.) of lungtuberculosis patients is no remarkable differences from the E. D. R. of healthy persons on the average, but seldom shows abnormal low value.
(2) The E. D. R. of old persons is efficiently lower than the one in young persons.
(3) The E. D. R. of the lungtuberculosis patients having been in sick for about 5 years is efficiently lower than the one of the patients having been is sick for within 3 years.
(4) The E. D. R. of the patients with increased blood sedimentatoin rate is a little lower than the one of normal persons, but the difference is not significant.
(5) The E. D. R. has no close connections with the breadth of pathogenic tissue. the evacuation's condition of bacills. bodytemperature, bloodpressure, menstruation and sex.
(6) The patients with the low E. D. R. shows a bad results for all the liver function tests, except the Azorubin S test, especially remarkable for the zinc turbidity test.
(7) No connections are observed between the blood picture, serum albumin and the E. D. R.
(8) In the first stage of pleurisy, the E. D. R. becomes showing low.
(9) Eosinopenia and low E. D. R. are observed in the most cases with the administration of SM and PAS.
(10) The E. D. R. is not changable with the injection of 500 times Tuberculin solution, and there are no connections between the E. D. R. and the strength of the skin reaction with the injection of 2000 times Tuberculin solution.
(11) The ratio of both the Uric Acid and Kreatinin in urine is steady by the injection of Adrenalin, and no connections with the F. D. R. also is not observed.

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I have measured the serum sodium and potassium in lungtuberculosis patients and estimat ed the ratio of both the sodium and potassium. Furthermore, I have made the Robinson-Kepler-Rower Test under the consideration of that the results of the water test must represent the Adrenocortical functions through the water metabolism. The results are as follows:
(1) In general, there are no differences on the adrenocortical functions between healthy persons and lungtuberculosis patients. The decline tendencies of Adrenocortical function are observed in some of the lungtuberculosis patients.
(2) The decline tendencies of Adrenocortical functions become increased with the ages, and become remarkable at the age of over 40.
(3) The decline tendencies of Adrenocortical functions have connection with the clinical symptoms, and become increased at the following conditions; having a longstanding process, increased blood sedimentatations rate, wide spreaded pathogenic tissue, fever, evacuation of bacillus in sputum, body weight below the standard weight and complication of intestinal tuberculosis etc., but no connections are observed with the follows; lun capacity, Chemical treatments and the intensity of zinc turbidity reaction and of Tuberculin skin reaction.

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The values of 17-Ketosteroid in the Urine of the lungtuberculosis patients was measured and was compared with the Thorn's test, the ratio of both sodium and potassium and the Robinson-Kepler-Power water test. The results are as follows:
(1) The excretion quantity of 17-Ketosteroid in the urine of the nontuberculosis patients at the convalescent stage are 7.58mg per day (4.66-16.5mg) on the average, and the value, less than 4.5mg daily, is thought as the abnormal low value.
(2) In the tuberculosis patients it shows 5.76 mg daily on the average and the value, less than 4.5 mg daily is 35 percentage.
(3) The decline of the exeretion in urine has a close connection with the age, increased blood sedimentation rate, breadth of pathogenic tissue, decreased lung capasity, body weight below the standard weight and the deciline of the liver functions, but no define connections are not observed with the process of the illness, bodytemperature, evacuation of bacillus and chemical treatments.
(4) Some of the various symptomes of lungtuberculosis, especially the age, increased blood sedimentation rate and intestinal tuberculosis as a complication etc. have a close connection with the value of 17-Ketosteroid in Urine, Thorn's test, ratio of both serum sodium and potassium and Robinson-Kepler-Power Water test.
(5) The decline of the adrenochrtical functions in lungtuberculosis is very light.

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I have studied the influences of the adrenocortical function on the clinical process by the treatments and the obstructive actions of the tuberculous bacillus on the whole blood in the lungtuberculosis patients, besides observed the obstrutive actions of the tuberculous bacillus multiplication in the whole blood of the rabbits with the removal of the adrenal glands or with the longstanding injection of ACTH. Furthermore, I have observed the rabbits with the inoculation of tuberculous bacillus pathologically and bacteriologically. The results are as follows:
(1) The clinical processes of the lungtuberculosis patients with the treatments are not influenced by the states of adrenocortical functions.
(2) There are no define connecions observed between the adrenocortical functions of the lungtuberculosis patients and the obstructive acions of the tuberculous bacillus multiplication in the whole blood by the slide cell culture.
(3) In the lungtuberculosis patients with the normal obstructive actions of the tuberculous bacillus multication in the whole blood, many cases show a good process with treatments.
(4) In the rabbits without the infection of lungtuberculosis, the obstuctive actionst of the human type tuberculous bacillus multiplication in the whole blood are not influenced by the removal of one side of the adrenal glands or the longstanding injection of ACTH, and the obstractive actions of the oxen type tuberculous bacillus multiplication is declined by the removal of one side of the adrenal glands and the half removal of other side.
(5) As the rabbits without any management, with the removal of one side of the adrenal glands and with the longstanding injection of ACTH are inoculated with tuberculous bacillus, the tuberculin reaction represents positive in the first cases and the pathogenic change is localized in the site of the inoculation, but it's reaction is strongly positive in the latter 2 cases and represents a caseaction early and highly.
(6) The number of the tuberculous bacillus in the pathogenic tissue of the site with the inoculation of the bacillus and the morphologic districution are not directly influenced by the removal of the adrenal glands and the longstanding injection of ACTH.

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I have measured the serum bilirubin of the lungtuberculosis patients with various types. The results are as follows:
(1) The total bilirubin in the healthy persons and patients having become positive to the tuberculin skin test within 6 months shows a little high level, but not efficent.
(2) The total bilirubin of the lungtuberculosis patients become decreased in the severe cases and become increased in the light cases, but it's increase is not efficient. The prosperity and decay of the indirect bilirubin follows in the one of the total bilirubin, but the direct bilirubin is not observed in the most of cases and it's prosperity and decay is parallel to the one of the total bilirubin when it is observed. But it become increased in the severe cases while the total bilirubin become decreased. Because of the reverse absorption by the connection with the pathogenic changes of the liver tissue.
(3) The total bilirubin become decreased in tPe gollowing cases, with active process, aggrabating symptomes, strict rest, cavity especially big cavity or maltiple cavities, remarkable body weight loosing, increased blood sedimentation rate, fever, positive bacillus in sputum, and aggravating leucocyte picture etc.
(4) The total bilirubin become most decreased in the exsudative type of the mixed phthisis and the next is the productive type of it.
(5) The total bilirubin of the lungtuberculosis patients with the complications especially intestinal tuberculosis shows remakably low, and it shows high in the cases with hemothorax or bloody phlegm, but it shows low in the ceses with the tendency of anemia.
(6) No connections are observed between the total bilirubin and the days of illness or/and the lung function.
(7) The total bilirubin is not influenced in the most of cases with the short period of various treatments, except it become decreased in the cases with the treatment of INAH and increased in the cases with the treatment of artificial pneumoperitoneum.
In the cases with the longstanding administration of BAS, the total bilirubin become increased as same as the cases getting well by other treatmtnts, but it is not changed in the cases without any changes of symptomes by the use of PAS. The direct bilirubin is not easily changed even on the cases having showed a good respondency to the treatments.
(8) The prosperity and decay of the serum bilirubin is not a characteristic of the prognosis in lungtuberculosia at the present time with advanced treatments.

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I have studied the reticuloendothelial systems and the liver function tests of the servere, moderate and light lungtuberculosis patients separately and observed the connections between the above results and the breadth of the pathogenic tissue, serum bilirubin. The results are as follows:
(1) Since the stand point of the congorot index in lungtuberculosis, the function of the reticuloendothelial systems become low and the total bilirubin also become decreased with the width of pathogenic tissue.
(2) Bromsulphalein test, azorubin test, phenothiazin test, hippuric acid test. galactose test, thymol turbidity test, total serum proteins, α and β-Globulin fraction, phenol turbidity reaction, urobilinogen in urine, reatinin in blood, rest nitrogen and Thorn's test etc. Sometimes show some connections with the bneadth of the pathogenic tissue, but no close connections with the prosperity and decay of the serum bilirubin was observed. Especially, there are no connections to exp; ain clearly that the decline of the total bilirubin is caused by the breadth of the Pathogenic tissue.
(3) Takata reaction has no connection with the breadth of pathogenic tissue and the prosperity and decay of the total bilirubin, but lugol reaction and zine turbidity test, especially the latter one, has a close connection with the breadth of the pathogenic tissue and they become showing positive and strong with the widening of the pathogenic tissue. At the same time, the total dilirubin shows a low level.
(4) The serum Albumin become decreased with the widening of the pathogenic lung fields while the globulin, especially γ-globulin become increased. In such the cases, the total bilirubin become decreased and it's connection is efficient.
(5) In the severe cases of lungtuberculosis with normal or increased kreatinin-clearance levels, the total bilirubin shows a low level.
(6) The decline of the total bilirubin is connected with the breadth of the pathogenic lung fields and also connected partly with the absorptive functions of the reticuloendothelial system, and it seems to be connected with the formation of hemoglobin on the stand point of the abnormality of protein metabolism, but seems to be not connected with the functions of liver celle and the kidney functions.
(7) The direct bilirubin does not show any connections with the various tests.

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I have studied the prosperity and decay of the serum bilirubin in the lungtuberculosis patients with the oral administration of 4g hemoglobin or the intravenous administration of 20% Glucose 20 cc daily for 10 days. The results are as follows: At the same time, the results of other several experiments are added.
(1) The total serum bilirubin become temporally increased about 5 hours after the administration of hemoglobin in both healty persons and patients.
(2) The total serum bilirubin of the lungtuberculosis patients with the administration of hemoglobin shows the most low level in the severe cases and it is parallel to the cases without the administration in the standpoint of the breadth of the pathogenic tissue. Judging from the congo-red index, it is high at below the 49, and is almost same at over the 49.
(3) The congo-red index is not changed after the administration of glucose, while the total serum bilirubin become remarkably decrased in the severe cases.
(4) The total serum bilirubin become increased by the administration of hemoglobin at the both periods befere and after the administration of glucose and it shows a little high level at the periods after the administration of glucise, but it become decreased with the widening of pathogenic tissue, especially remarkable in the severe cases. But the increased level of the serum bilirubin by the administration of hemoglobin is remarkable in the cases with administration of glucose especially in the severe cases, as the serum bilirubin in the cases without the administration of hemoglobin become decreased by the administration of glucose.
(5) In the sever cases of lungtuberculosis, the dosis of blood plasma shows increased and it is thought as one of causes for hypobilirubinemia.
(6) Temporary hypobilirubinemia occurs by the administration of INAH, but it's cause is still obscure.

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1. The author has made researches, in summer, autumn and winter, of the quantitative variation, following the time after death, of the non-protein nitrogen in bones and cartilages, and the anorganic phosphoric, sulphuric and nitric acid in brains, lungs, livers, kidneys and spleens of rabbits.
2. Each quantities of the non-protein nitrogen in bones and cartilages increase following the time for a certain period after death. The quantities of the anorganic acids in these five organs increase similarly following the time after death, and then decrease after a certain period.
3. The author has proved that the disintegration of dead bodies is more speedy 8-11 times in summer and 3-4 times in autumn than in winter.

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Ever since Takahara discovered acatasemia in 1946, many studies on the questions concerning causative factors of progressive necrotic ulcers and phsiological significance of catalase have been made public.
As there is no report other than that by Miyamoto concerning the relationship between blood of the acatalasemic patient and bacteria and considering this to be an interesting problem, the author conducted a series of experiments with a view to elucidate actions of several bacteria belonging to Streptococcus and Pneumococcus on blood of the acatalasemic patients.
Namely, the author carried out aerobic and anaerobic cultures with the medium containing Diprococcus pneumoniae I, II, III, Streptococcus hemolyticus, or Streptococcus viridans, which was loaded with either acatalasemic blood or blood of the normal person, and other series of cultures containing each of the these media loaded still further by inoculation of other medium containing either KCN or hydroxylamine (inhibitory agent of catalase action).
By macroscopic and spectroscopic observation on the results of above mentioned expeirments, the following findings were obtained.
1) As regards the rate of bacterial growth under either condition no great difference could be recognized between the medium loaded with normal blood and that with acatalasemic blood.
2) In the cases of aerobic culture, the production of Methb and decolorization in the media containing actalasemic blood tended to be more marked than those in the media with normal blood.
3) In the cases of anaerobic culture, the production of MetHb and decolorization phenomenon decreased in the media containing acatalasemic blood.
4) In the aerobic culture with the medium loaded with inhibitory agent of catalase action, the rate of MetHb production and decolorization phenomenon were about the same in both the medium with normal blood and the medium with patient's blood. While, in anaerobic cultures influences of addition of these agents could hardly be discerned.
5) Since the blood of patient doss not contain catalase, the production of MetHb and decolorization phenomenon in the medium containing acatalasemic blood seem to have occured due to influences of hydrogen peroxide (H₂O₂) produced by bacteria.

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As previously reported in Part I the author found that hemoglobin had tendency to show the production of MetHb and the decolorization remarkably when Streptococcus hemolyticus, Streptococcus viridans, and Diprococcus pneumoniae I, II and III were cultured in the medium using the blood of acatalasemic patients.
These changes clearly have indicated that the blood of these patients lacking in the catalase is unable to dispose of hydrogen peroxide (H₂O₂) produced by bacteria and subsequently Hb is oxidized to form MetHb and with progress of oxidation the constituents of the blood seem to turn to decolorization substances such as propentodyopent.
This time with a view to clarify this point still further, the author studied action of still bacteria en erythrocytes of the normal and the patients, and from the results of this study arrived at the following conclusion.
1) Diprococcus pneumoniae II, III and Streptococcus viridans markedly accumulate H₂O₂ during oxidation process of glucose.
2) When glucose is used as substrate loaded with acatalasemic erythrocytes and shaken, in the case of Diprococcus pnemoniae I, II, or Streptococcus viridans, a marked production of MetHb has been observed, when normal erythrocytes are loaded, the production of MetHb has been extremely small as compared with each of these bacteria in the case of the acatalasemic erythrocytes.
When pyruvate, succinate, or aspartate is used as substrate, the influences of each of these bacteria on erythrocytes are on the whole quite small.
3) Productivity of MetHb and decolorization by bacteria have a parallel relationship with the accumulation of H₂O₂.
4) Using glucose as substrate, influences of Diprococcus pneumoniae II on the respiration of erythrocytes are as follows.
a. In the case where erythrocytes are not loaded (the control) O₂-consumption decreases by 60-90 minutes.
b. In the case where acatalasemic erythrocytes are loaded, so long as Hb exists, O₂-consumption continues to rise.
c. In the case where normal erythrocytes are loaded, up to 60 minutes O₂-consumption is comparatively lower than that of the control but it does not fall even 120 minutes later.
d. On examining H₂O₂ in solution after these reactions, an extremely minutes quantity of it has been traced in the case of loading acatalasemic erythrocytes, while none can be traced in the case of loading normal erythrocytes.
e. Thiourea and cysteine have been found to completely recompensate whatever influences exerted upon O₂-comsumption of bacteria by acatalasemic blood.
5) After studying color changes of the erythrocytes to which H₂O₂ of various concentrations had been adden, the results thus obtained were quite identical with those which erythrocytes had been influenced by respiring bacteria.
6) From these faets as far mentioned it may be assumed that the influences of respiring bacteria upon the acatalasemic erythrocytes are due to the action of H₂O₂ produced by bacteria.

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1. The respiration of mitochondria was measured by the Warburg's manometer and the intermediates of the TCA cycle were used as the substrates.
2. The O₂-uptake of the normal rabbit brain mitchondria is about twice of the rabbit mitochondria with latent cerebral local anaphylaxis.
3. On the stimulation of the substrate;
The succinate, α-ketoglutarate, oxalacetate and pyruvate accelerate the O₂-uptake in both cases, but the O₂-uptake of the normal brain is always more than that with anaphylaxis.
4. Fumarate stimulates the O₂-uptake of the normal brain, but does not stimulate that with anaphylaxis.
5. Citrate and malate do not accelerate in both cases.

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The effects of Diamox on the water content in cats brain were measured.
The experiments were performed by Hatschek's cobalt-chloride method taking the samples at 9 and 24 hours respectively after the injection of Diamox, in which the period of the former showed the strongest chenges and the latter showed nearly normal (the latter came back to nearly normal value) in the preliminary experiments.
Concerning about the mean values of each 6 cases, the mhole water content showed 2% decrease, the free water content showed 3.4% decrease and the combined water content showed slightly decrease at 9 hours then the normal, although these water contents showed normal values at 24 hours.
From these experimental results, the anticonvulsive effects of Diamox were considered to be caused by the strong dehydrating action in the brain.

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The effects of Diamox on the free amino nitrogen in cats brain were measured
The experiments were performed by Van-Slyke method taking the samples at 9 and 24 house respectively after the injection of Diamox.
Concerning about the mean values of each 8 cases, the free aminonitrogen showed 25.9% increase at 9 hours, while 37.3% decrease at 24 house than the normal values.
The anticonvulsive effects of Diamox were considered to be based on the ammonium management accompanying with the increases of glutamic acid.
On the contrary, the decreases of the free amino nitrogen were considered to be based on the lack of the sources of glutamic acid synthesis.

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The effects of Diamox on the cholinesterase activities in nucleus lenticularis of cats brain were measured.
The experiments were performed by Ammon method taking the sanples at 9 and 24 hours respectively after the injection of Diamox.
Concerning about the mean values of each 7 cases, the cholinesterase activities showed 44.8% increase at 9 hours and 43.4% increase at 24 hours.
The anticonvulsive effects of Diamox were considered to be caused by the acceleration of the acetylcholin decomposition of cholinesterase from these experimental results.

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The effects of Diamox on the CO₂ content in the circulatory arterial blood with Dial anesthetized cats brain were measured.
The experiments were performed by Van Slyke Saito method taking the blood at 9 and 24 hours respectively after the injection of Diamox.
Concerning about the mean values of each 8 cases, the CO₂ content showed 11.1% and 14.1% decrease respectively than the non-injected group.
These experimental results were not so sufficient for the explanation of the anticonvulsive effects of Diamox, that the author would like to give a report of the experimental results here.

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The effects of Diamox on Cr K. E. G. and Hg K. E. G. of the nerve cells in cerebral cortex and Nissl gray of cats brain by Hamazaki K. E. G. method.
Concerning about the (experimental) results of each 6 cases, on Cr K. E. G., the granula increased as approximately twice as much in both of the nerve cells and Nissle gray at 9 hours and then gradually decreased at 24 hours, but they were still suspected to be much more than the normal, while Hg K. E. G. showed no remarkable changes after the injection.
This increasing of granula is considered to be caused by acceleration of metabolism in the brain.

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One hundred and fifty-nine normal premature dogs weighing about 1.5 to 3.0 kg., fed under the same conditions were used. Incision, contused wounds and cauterization were made over the oral mucosa by means of incision knife, curet, and electric cautery.
Then the progress of the vascularization of blood vessels in each of these wounds was observed during the period of 14 days immediately after making the wounds. After being treated with Imagawa's Guttapercha Infusion Method, small pieces of tissue were taken these wounds 12 hours, 24 hours, 3 days, 7 days, and 14 days after making the wounds, and transparent specimens were prepared from these pieces for the steroscopie observations of the changes of blood vessels with a binocular microscope, while a part of these tissues was stained with haematoxylin-eosin by the rontine paraffin method for histological obrervation. The results obtained are as follows:
a) The fundamental principle of the vascularization in the process of wound healing of the buccal, lingual and gingival mucosae, was quite same regardless of the kind of wounds.
b) The vascularization of injured regions was most rapid on the lingual mucosa in all cases of the wound, being followed by that on the gingival and on the cheek in the order.
c) Disintegration of capillary networks due to successive degeneration in the early stage was most remarkably noticed in the case of cauterization and was decreased in the case of contusion and incision in the order and thus the regeneration was delayed.
d) The vascularization of injured regions of the gingiva was progressively affected by the alveolar bone and the blood vessels of the periodontium at the alveolar margin and it also played the most important role in the blood supply for the gingiva.
e) In the cases of contusion and cauterization the vascularization of the gingiva showed a marked difference in their rate according to the injured regions. The vascularization had the following five variations iu its type: ellipse, triangle, inverted triangle, trapezoid and deepening or atrophy of gingival margin, showin a regularly definite pattern in each wound in its healing process.
f) In the case of gingival detaching its margin showed a recovery up to the preoperative level and no formation of deep gingival pocket could be noticed. A tendency of regeneration of alveolar bone and re-attachment of epithelium to the cementum was seen on the fourteenth day and the periodontal capillaries played a very important part in vascularization of blood vessles of the gingival margin.
g) The results from the experiments of the influence of Parotin on the regeneration of blood vessels could be summarized as follows:
i) Vascularization of the injured region was most rapid in case of Parotin injection in normal premature dogs, being followed by the control cases and the cases of Parotin injection and removal of parotid gland. Vascularization was remarkably delayed in case of removal of parotid glands.
ii) Injectione of one mg./Kg. of Parotin stimulated progrssively the vascularization of supporting structures of the teeth and the scar formation histologically.

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Citric acid conecnetrations in sera and cerebrospinal fluids of various psychotic patients and of normal persons were determined by Natelson's method; and the following results were obtained.
Citric acid concentrations in serum: In comparing the citric acid concentrations in sera of various types of schizophrenic patients with that in serum of normal persons, it has been found that in hebephrenic form it is lower than in the normal, while in catatonic form it ranges widely from higher to lower, and in paranoid form it is nearly the same as that in the normal. In depressive form it tends to be lower than that in the normal. A marked decrease in the concentration has been observed both in chronic and deteriorated schizophrenia as well as in stupor, whereas a marked increase can be recognized in excited state.
Citric acid concentration in cerebrospinal fluid: Citric acid concentration in cerebrospinal fluid has been found to be 2 or 3 times that in serum; and the value in the cerebrospinal fluid of lumbar region is greater than that in cerbral region. The concentration in the cases such as cerebrovascular disease and postlobotomy cases show an increase, whereas in hydrocephalus it is slightly lower.

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The author carried out periodically follow-up studies on the changes of citric acid concentration in serum of various psychotic patients after oral administration of glucose, saccarose and food; and obtained the following results.
After the oral administration of glucose, those that showed abnormal responses, namely, delayed responses, were the chronic schizophrenic and the acute excitator schizophrenic patients. Since these abnormal responses are observable in diabetic patients as well as in the children with fits of convulsion, from the standpoint of the citric acid metabolism it is assumed that there is some correlation between these two groups mentioned above.
In the case of oral administration of saccarose, a transient increase of the citric acid concentration in blood can be recognized contrary to the case in glucose administration. Abnormal response has been recognized in one of acute excitatory schizophrenic patients.
After taking food, a certain number of shizophrenic patients show abnormal nesponses.
From these findings, in schizophrenic patients biochemically no metabolic abnormalities can be recognized at the onset of disease, but with gradual chronic deterioration irreversible metabolic abnormalities seem to occur.

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1) In the cases who received electroshock treatment, a rapid increase in the citric acid concentration of blood has been recognized, showing on the average an increase of 30 to 40 per cent, with the maximum of 80 per cent. However, the value returns to the pretreatment level after one and a half hours to two hours, but no marked change in the citric acid concentration can be recognized in urine.
2) In the cases given insulin shocks, the citric acid concentration in blood during coma shows a decrease of 20 per cent and shows an increase on regaining consciousness, in the great majority; but there are some cases which do not show the decrease during coma. In the course of insulin treatment the value immediately before the next injection is lower than the normal value.
3) Subcutaneous injection of epinephrine increases the citric acid concentration. However among the schizophrenic and the depressive patients there are some who do not show an increase.
4) Successive adminstration of chlorpromazine decreases the citric acid concentration of blood. When glucose is given to the patients receiving the successive chlorpromazine administration, these patients show responses similar to the abnormal responses observable in the chronic schizophrenic and diabetic patients.
5) Oral adminstration of barbital shows the minimum value of the citric acid concentration in blood two to four hours after the administration.

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(1) The search was made to differentiate the possibilities of inducing the neuroallergy by infection in the case of polyradiculoneuritis with viruses of coxsackie group, strain Okumoto (A-2), Dohi (A-19) and Saragai (B-1) which were isolated from the patients with the polyradiculoneuritis showing Guillain-Barré Syndrome.
(2) (A) Previous repeated administration of these three strains of coxsackie virus in the adult mice through intraperitoneal route and the challenge of identical virus intrathecally.
(B) Similar repeated administration of PD₅₀ of poliovirus (Mahoney or Lansing strain) through intrathecal route and the challenge of coxsackie virus Okumoto Strain through same route to survived and sensitized mice after previous inoculations.
The observation for 1 month revealed no significant signs of paralysis. Histologically no discernible picture of polyradiculoneuritis in the spinal root, ganglia, nerve and the ischias was clarified.
(3) No acceptable evidence of the presence of neuroallergy by infection experimentally made in the above strains was obtained through the trials with coxsackie virus and polio virus in the mode of repeated inoculations.

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Certain results were obtained to illustrate the possibility of inducing the radiculoneuritis in experimental polio infections in mice.
(1) No remarkable inflammatory changes in the spinal root, ganglia, nerve and the sciatic nerve in the adult mice paralysed by intrathecal infection with poliovirus (Mahoney & Lansing strain), and no histological difference between two strains.
(2) The previous repeated administration of poliovirus (Mahoney & Lansing strain) intrathecally in the dosis of PD₅₀ (10^-4, 10^-5) and 10^-7 or intraperitoneally in the dosis of 10^-1 and the challenge of same virus in the dosis of 10^-2. 10^-4-6 intrathecal route revealed the mice with paralysis and without visible paralysis. In both mice no detectable histological pictures of polyradiculoneuritis in the spinal root, ganglia, nerve and the sciatic nerve were seen.
(3) The reproducible data would seem to indicate the discernible points that the poly-radiculoneuritis with Guillain-Barré Syndrome is not affiliated in the major part to the primary affection or repeated inoculations of poliovirus of the neuroallergic basis of pathogenesis.

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