(1) The grade of the intensity of brain swelling observed in clinical symptoms and electroencephalogram was the highest in the cases with resection of the cortex and then decreased in the order of those with brain tumors etc., with explorative craniotomy and with lobotomy. The cases with air insufflation by ventricular or lumbar puncture, however, showed almost no changes in electroencephalography.
(2) The decrease of the average number of alpha waves per second and the increase of the delta index became most striking in 1-4 postoperative days, then gradually recovered and returned to normal in 15-16 postoperative days, in the epileptics with coritcal resection.
(3) The brain swelling was generally marked on the side of craniotomy, and more marked at the part of craniotomy and less at the distant part, with consideration of the frontal domination.
(4) The electroencephalographic findings in the brain swelling, such as the changes of the delta index and alpha waves, were parallel with the clinical symptoms. But their appearance as well as their cessation were left a little behind the clinical symptoms.
(5) The electroencephalographic changes were not always marked at the part of craniotomy. This is due to the characteristic fact that the frontal lobe reacts more actively i. e. the frontal domination.
(6) The water content of the brain was measured in one case of autopsy, and the brain edema was ascertained to be more marked on the side of craniotomy than the other side.
(7) The electroencephalographic findings represent the clinical features of the brain swelling and have enough value for clinical application.

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A case of malignant myogenous tumor originated on the left spermatic cord of 49 aged man is presented. This tumor was found as a painless tumor in the testicular region about 7 years before the death of patient, and one year later it grew to a child-head sized tumor showing a rapid development. Clinically the tumor was diagnosed as a cancer of the testis and resected surgically. (Fig. 1)
No metastasized tumor was recognized at that time. However, 3 years and a half later a new tumor appeared on the center of the heald wound. The relapsed tumor was resected again, but after about one year and a half a tumor reappeared on the same region (Fig. 4) accompaning with an extensive metastasis in the thoracic cavity. (Fig. 8) The patient died by heart failure caused by the metastasized tumors.
Anatomical and histopathological observations revealed that this tumor was a leiomyosarcoma originated in the lower region of the left spermatic cord. The tumor tissues consisted of whorled masses of polymorphic spindel-shaped cells with lod-shaped mucleus similar to the immature smooth muscle cells. (Fig. 9 & 7)

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I reported in the 1st chapter the fact, that tle specific increase of each leukocyte is evidenced in the peripheral blood, bone-marrow and in the lymphatic tissue of the normal rabbit or guinea-pig injected with the extract from pseudoeosinophil, eosinophil or lymphatic leukocytes. In this chapter, I, employing such animals as have undergone either splenectomy, liver parenchymal disturbance, or reticuloendothelial blockade, examined to get certain detailed notions as to their functions, from which, results as follows were obtained.
1) When the extracts out of the pseudoeosinocytes or eosinophil leukocytes have been applied to splenectomized or disturbed liver parenchy animal, an increase of each leukocyte was induced respectively, same as was seen in 1st chapter.
2) When the pseudoeosinocyte extract or eosinophil leukocyte extract was applied to animals of reticuloendothelial blockade, different from the case in 1st chapter, a feeble sort of increase took place in case of pseudoeosinocyte extract, while with the latter, no brieftime increase has appeared, but only when 24 hours have lapsed.
3) If lymphocyte extract was injected in animals with splenectomy, liver parenchymal disturbance or reticuloendothelial blockade, though the was seen no blood lymphocyte increase, same remarkable proliferation as stated in 1st chapter was detected.

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In the previous chapters, I have described the so-called direct functions indicated by pseudoeosinocytes, eosinophil leukocytes, as well as lymphocytes, at the same time examining their mechanism. In this, I am going to discuss the case in which the above mentioned leukocytic extract has been added to the tissue culture of the bone-marrow and lymphnodes.
1) In case pseudoeosinocyte extract are added to any tissue culture of rabbit's bone-marrow, an improvement of tissue growth rate (i.e., an increase in pseudoeosinocytes) as well as an excitement in their wandering velocity are achieved in comparative particularity.
2) If I add lymphocytic extract to a rabbit's lymphnode culture, there happens in comparative unique mode, an improvement in the tissue growth rate (i.e., lymphocytic increase), and the lymphocyte rises in its wandering velocity.
3) If I add guinea-pig's eosinophil leukocytes extract on the culture of the same animal's bone-marrow, it induce an increase of eosinophil leukocytes in the growth zone, together with a rise in the wandering velocity of the same.
4) I evidenced that the direct influence of leukocyte extract explained in 1st and 2nd chapter, acts on the bone-marrow and lymphnodes in a direct manner.
Conclusions. Owing to those ezperimental results obtained throughout these chapters, I can conclude as folllows: i.e. The extracts stimulate the bone-marrow or lymphnode directly with comparative specifity, no matter how the liver and spleen might stand, and induce the increase of each sort of leukocytes respectively and of their wandering velocity so that they appear in the peripheral blood in short time, though it is better that the reticuloendothelial system is normal.

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Washing of organisms by phosphate buffer results in the decrease of their respiration, and the addition of divalent metal ions such as Mg₊₊, Fe₊₊, Mn₊₊ restores the decrease.
This study is performed to determine the mechanism of action of such metal ions by observing the influence of these ions on the metablism of metal ion deficient organisms; some strains of Sh. flexneri, Sh. dysenteri, S. typhi, Staphyl. albus, Staphyl. aureus, Staphyl. citreus, E. coli, Pseudomonus aeruginosa.
(1) The respiration in which glucose or pyruvate are substrates can be accelerated by metal ions and moreover in the case of Sh. dysenteri 3, succinate is the substrate in which respiration is accelerated too.
(2) The action of metal ions seemed to cause the reaction to perfect oxidation and in the case of Sh, dysenteri 3 succinate → fumarate reaction is accelerated too.
(3) It is concluded that metal ions act on enzyme system concerning phosphorus metabolism coupled with oxidation and this action results in the acceleration of respiration.

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This study is carried out to determine the mechanism of mutual action between two substrates which act to accelerate bacterial respiration by their mutual action.
From the results of some experiments with some strains of B. dysenteriae, such as Sh. dysenteri 3, Sh. flexneri 1. Sh. flexneri 3, two types of mechanism of the action was discovered, they are as follows.
(1) In the case of succinate and aspartate, or succinate and glutamate, these combinations of substrates can accelerate the respiration as compared in the case of each substrate alone. The mechanism of this mutual action included aspartate _??_ glutamate reaction.
(2) In the case of couples in which one substrate is glucose, pyruvate, or succinate and another is tartarate, citrate, histidine, tryptophane, tyrosine, or lysine, these couples of substrates can acceletate the respiration too, in spite of the fact that the latter substrates cannot be oxidized when they are added alone to organisms. The mechanism of this mutual action includes the phosphorous metabolism coupling with oxidation, and addition of the substrates such as tartrate, citrate, etc. to glucose, pyruvate, etc. seemed to activate ATP _??_ ADP reaction.

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An experiment was conducted on the attitude of some strains of B. dysenteriae on citrate and the following results were obtained:
1. Citrate, independently, as substrate, was added to organisms but development of CO₂ and O₂ uptake was not found. Also disappearance of citrate from medium was very little.
2. The disappearance of citrate in medium was accelerated by addition of glucose, pyruvate, succinate, or ATP. From this, it can be concluded that in order for citrate to be analyzed by B. dysenteria, step in which citrate can be activated is required: the energy necessary is believed to be furnished by glucose, other added substrates or ATP.
3. Whether citrate requires energy to pass cell membrane could not be made clear, nor the pathway of metabolism.

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An experiment was conducted to consider the metabolism system of histidine, tryptophane, tyrosine of some strains of B. dysenteriae. The following results were obtained:
1. With the B. dysenteriae used, when these amino acids were independently substrated, no analysis took place, but when added with glucose, pyruvate, succinate or ATP, they were oxidized.
2. Under these conditions, it is believed that amino acids analyze first by oxidative deamination of α-amino group, then by decarboxylation.
3. In this case it is believed that glucose, and other added substrates or ATP furnish the energy necessary for the activation of α amino group of these amino acids.

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Convulsive seizures were caused by metrazol injection in cats, and the water content of the brain was measured at the cerebral gray matter, white matter, brain stem and cerebellum by the cobalt-chloride method.
The water content became a little more at the preconvulsive stadium and it became the most at the acme as well as the postconvulsive stadium. Then it showed a gradual decrease to the normal as time elapsed.
The preconvulsive increase of the water content could be considered to cause the convulsion, and the convulsion could also be considered to cause the increase of the water content, because of the maximal increase at the acme and postconvulsive stadium, which suggested some relation between the water content and the activity of the central nervous system.

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The ketoenolic granules were investigated in the motor cortex and the results were as follows:
1. No change was observed at the preconvulsive stadium.
2. The granules in the nerve cells, especially in the Betz's giant pyramidal cells decreased or disappeared and those in the Nissl's gray substance rather increased at the acme and the postconvulsive stadium.
This fact suggested the ketoenolic substance moved from the ganglion-cells to the Nissl's gray substance or to the nerve fibers at the moment of such great consumption of energy as convulsion. And this change recovered gradually to the normal as time elapsed, which was similar to the above-stated changes of the water content in the brain.
It was interesting that this change was most striking in the Betz's cells which were considered to have very close relation to convulsion.

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