Okayama Igakkai Zasshi (Journal of Okayama Medical Association)

A case of living related liver transplantation for an adult recipient with primary biliary cirrhosis

Takahito YAGI, Naoto URUSHIHARA, Masahiro OISHI, Hiroyoshi MATSUKAWA, ...

1998 Volume 110 Issue 1-6 Pages 1-8
Published: June 25, 1998
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.110.1-6_1

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A 49-year-old female received a living related liver transplantation from her son. She had a 13-years history of primary biliary cirrhosis (PBC). The preoperative 1-year survival rate and mortality after 6 months were calculated to be 6.5% and 99.3%, respectively. The graft included left bisegments weighing 435g, and was equal to 43% of the calculated standard liver volume. Massive ascites were sustained due to accompanied portal hypertension. On postoperative day 7, she manifested massive tarry stool by portal-hypertensive colonopathy. The CT scan revealed a right subphrenic abscess on the postoperative day 14. After drainage of the abscess, the serum total bilirubin level decreased to the norman range. She was discharged with extracorptreal tube drainage.
On the postoperative day 169, she was readmitted due to acute elevation of the serum bilirubin level. Echography revealed selective dilatation of the hepatic bile duct in the segment 2, and suggested the presence of insufficiency of hepatico-jejunostomy in the early postoperative phase. Despite emergent PTCD, jaundice was sustained and responded poorly to steroid pulse therapy. According to tapering of oral prednisolone, the serum bilirubin level gradually decreased and reached a the plateau between 2 and 4mg/dl. The patient is currently completely free from oral steroid therapy and in receiving interventional hepaticoplasty using the YAG laser system.
We concluded that living related liver transplantation is an effective transplantational option for adult end-stage liver disease.

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Localization of Mn- and Cu/Zn-SOD-like immunoreactive cells in the rat brain

YAN Wang

1998 Volume 110 Issue 1-6 Pages 9-20
Published: June 25, 1998
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.110.1-6_9

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The localization of manganese (Mn) and copper-zinc (Cu/Zn) superoxide dismutase (SOD)-like immunoreactive (lir) cells in the normal rat brain and the change in numbers of both SOD-lir cortical cells after the injection of ferric chloride into the sensorimotor cortex were investigated immunohistochemically. Large and medium-sized cell bodies, pyramidal to polygonal in shape, were immunostained by anti-Mn-SOD antibody. Most of these were not immunolabeled by anti-GFAP antibody, suggesting that most of the Mn-SOD-lir cells are neurons. These cell bodies were distributed in some selected brain areas with different densities. The densest population of Mn-SOD-lir cells was found in the hypothalamic and limbic structures, and in some brain stem nuclei, such as the thalamic reticular nucleus, the locus ceruleus and the pontine nucleus. Anti-Cu/Zn-SOD antibody immunostained many small round cells throughout both white and gray matter, most of which were also immunolabeled by anti-GFAP antiserum. After intracortical injection of FeCl₃, the numbers of Mn- and Cu/Zn-SOD-lir cells per unit area within the adjacent narrow region of the injection site significantly increased 2 and 6 hr after the injection, respectively.

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Experimental study on the maintenance of hepatic circulation with arterial blood through a catheter bypass during total vascular clamping at the hepatic hilus

Takashi MORI

1998 Volume 110 Issue 1-6 Pages 21-29
Published: June 25, 1998
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.110.1-6_21

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This study was designed to determine the safe range of the arterial blood flow rate into the portal vein through a catheter bypass under the condition of total vascular clamping at the hepatic hilus in mongrel dogs. A control group and 6 experimental groups were prepared according to the arterial blood flow rates through the bypass, 0, 10, 25, 50, 75 and 100% of the normal total hepatic blood flow volume. After 2 hours of bypass circulation, the dogs were sacrificed and the hepatic tissues of all groups were examined by light microscopy and transmission electron microscopy. Compared with the control group, ischemic changes were observed in the 0% and 10% groups, while there were no particular changes in the other groups. Edema in the Glisson's sheath was observed in the 75% and 100% groups, while there was no edema in the other groups. The results demonstrated that the lower limit of the arterial blood flow rate through a catheter bypass to mantain the hepatic structure was between 10% and 25% and the higher limit was between 50% and 75% of the normal total hepatic blood flow volume.

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2β-(3-Hydroxypropoxy) 1α, 25-Dihydroxyvitamin D₃(ED-71), increases bone mass by stimulating bone formation in hypophosphatemic mice

Hideaki OCHI

1998 Volume 110 Issue 1-6 Pages 31-37
Published: June 25, 1998
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.110.1-6_31

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To determine the effects of ED-71, a new active vitamin D₃ analogue, on bone, we examined the action of ED-71 and 1α, 25(OH)₂D₃, using hypophosphatemic (Hyp) mice, a model for X-linked hypophosphatemic rickets in humans. ED-71 has the unique characteristics of a longer half-life in serum, a higher binding affinity to serum vitamin D binding protein and lower affinity to the vitamin D receptor than 1α, 25(OH)₂D₃. Hyp mice were treated with 10-1200 pmol/kg/day of ED-71, 40-400 pmol/kg/day of 1α, 25(OH)₂D₃, or the vehicle alone, administered daily for 21 days by subcutaneous injection. ED-71 at doses of 100-400 pmol/kg/day, dose-dependently effects in increased bone size and bone mineral density (BMD) without causing hypercalcemia. However 1α, 25(OH)₂D₃ at a dese of 400 pmol/kg/day, demonstrated only slight increases in bone size and BMD with hypercalcemia. These findings suggest that ED-71 may be more beneficial in the treatment of X-linked hypophosphatemic rickets than 1α, 25(OH)₂D₃.

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Analysis of ictal EEGs in West syndrome

Takashi ASANO

1998 Volume 110 Issue 1-6 Pages 39-51
Published: June 25, 1998
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.110.1-6_39

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Although tonic spasms (TS) observed in West syndrome (WS) are regarded as generalized seizures, the mechanism of their occurrence has not yet been fully elucidated. We analyzed ictal EEGs of TS to determine the mechanism and to clarify the role of cortical pathology in the occurrence of TS.
Of 53 infants with WS, we classified cryptogenic cases as Group I. Symptomatic cases were classified into 2 groups: Group II consisted of those without partial seizures during the period of WS and Group 3 consisted of those with partial seizures.
We analyzed 7283 ictal EEGs of TS regarding the following 5 points: 1) patterns of ictal EEGs, 2) frequency of isolated TS, 3) asymmetry of ictal EEGs, 4) spasm-spasm intervals, and 5) duration of ictal EEGs of each TS.
We concluded that the asymmetry of ictal EEGs and the “rhythmic slow activity” immediately following ictal EEGs of TS were related to cortical pathology. Since “rhythmic slow activities” were often observed in Group III and appeared at the same regions as those in ictal EEGs of concomittant partial seizures, they were considered to be peculiar EEG patterns related to cortical pathologic lesions.

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Sugar chain alterations of glycoproteins in spent culture media of human hepatocellular carcinoma cell lines analyzed by lectin-affinity electrophoresis

Masako FUKAZAWA

1998 Volume 110 Issue 1-6 Pages 53-60
Published: June 25, 1998
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.110.1-6_53

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Sugar chain alterations of α-fetoprotein (AFP), α₁-Antitrypsin (AAT) and transferrin (Tf) in spent culture media of human hepatoma cell lines, HuH-7, huH-1, Hep-G2 and KIM-1, were analyzed by lectin-affinity electrophoresis with Concanavalin A (Con A), Lens culinaris agglutinin (LCA) and Phaseolus vulgaris lectin (E₄-PHA) and antibody-affinity blotting. Their glycoforms demonstrated lectin-dependent characteristic patterns similar to the glycoforms of AFP in hepatocellular carcinomas, although their separation became less chear as the number of sugar chains per molecule increased. The proportions of band intensities of the glycoform of AAT and Tf corresponding to AFP-C1, AFP-L3 and AFP-P4 increased consistently in all cell lines. However, the extent of the increases in the glycoforms differed from one cell line to another, reflecting tumor heterogenity. On the other hand, the sugar chain alterations of glycoproteins were similar in each cell line, although the glycoforms separated with Con A showed slightly different patterns from those separated with LCA. Accordingly, hepatocelluler carcinoma may be detected at an early stage by analyzing the glycoforms of serum AAT and Tf by lectin-affinity electrophoresis for cases with negative AFP.

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[title in Japanese]

1998 Volume 110 Issue 1-6 Pages 61-69
Published: June 25, 1998
Released on J-STAGE: March 30, 2009

DOIhttps://doi.org/10.4044/joma1947.110.1-6_61

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[title in Japanese]

1998 Volume 110 Issue 1-6 Pages 71-91,93
Published: June 25, 1998
Released on J-STAGE: October 29, 2012

DOIhttps://doi.org/10.4044/joma1947.110.1-6_71

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