In the previous study, Sr
89 was administered to cocks to ascertain the effect of internal irradiation on the plasma protein fractions, and the temporary and slight decrease of albumin in relative percent, was observed. Further study, using Ta
182 was made by a similar method. The employment of Ta
182 is based on the fact that tantalum is taken up chiefly by the liver. In the present experiment, Ta
182 (Potassium tantalate) was administered to 4 cocks, subcutaneously, in doses of 0.05 to 1.5 μc per gram of body weight, and the plasm proteins were analyzed by the Tiselius electrophoretic method. The electrophoretic analyses were carried out in phosphate buffer (pH 7.8, ionic strength 0.144) under the conditions of 5 mA, 60∼80 V, and 80 minutes. The results obtained are summarized as follows. 1) After the administration of Ta
182, the albumin decreased, relatively. The particular electrophoretic patterns, showing the peak of albumin lower than that of globulin (φ
γ), were often obtained in the case of injection of 0.5 to 1.5 μc/g. 2) The relative increase, corresponding to the albumin decrease, was found in all the globulin fractions such as α
1, α
2, β, φ, and γ. In other words, the increasing components were not restricted to the specific fractions. 3) As the result of the administration of Ta
182, the rising in the red and white cell counts was pointed out, and in this augmentation of white cells the heterophylic leucocytes played the leading role. 4) Regarding the tantalum distribution in the various organs of the cock, the liver and lung contained the highest radioactivity, followed by the kidney, spleen, testicle and bone marrow, respectively.
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