The Japanese Journal of Veterinary Science
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
Volume 41, Issue 4
Displaying 1-11 of 11 articles from this issue
  • Masami MOCHIZUKI, Shin-ichiro KONISHI
    1979 Volume 41 Issue 4 Pages 351-359,362
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    Two strains of a virus were detected in feline kidney and liver cell cultures derived from an apparently healthy kitten. They replicated in feline cells with vacuolation, and multinucleated giant cell and small plaque formation. They were principally cell-associated and required actively dividing cells for their replication. From their viral nucleic acid, they were presumed to be of RNA type. They were sensitive to ether, heat and acid. The smallest infective particle was determined to be between 100 and 130 nm in diameter by membrane filtration and electron microscopy. They had a buoyant density of 1.19 g/ml in sucrose. They were identified as feline syncytial virus (FSV) by their biological and physicochemical properties, and serologically by the serum-neutralization and microimmunodiffusion tests. In a decade from 1968 the incidence of FSV infection in cats was very low (3.8%) in the Tokyo area. FSV is, however, of particular importance for investigators and vaccine producers who use cats and feline cell cultures also in Japan.
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  • Muneo NAKAZAWA, Hisashi NEMOTO
    1979 Volume 41 Issue 4 Pages 363-367
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    The hemolytic activity of Moraxella bovis was investigated. It reached a maximum level in the initial stage of the logarithmic phase. This level was maintained for at least 120 hours during which the shaken culture of M. bovis in trypticase soy broth had been examined sequentially. Hemolysin was inseparable from the bacillary body, because after filtration through 0.45 μm Millipore filter or centrifugation at 8, 000 rpm, no hemolytic activity was proved in the filtrate or supernatant. No difference was demonstrated in the susceptibility of erythrocytes to this hemolysin among several species of animals. The hemolysin of M. bovis was activated by Ca++, but not by any reducing agent, and destroyed by heat, formalin and trypsin. In seven of 8 sera collected from clinically infected calves, a distinct increase of antihemolytic antibody titer was proved. Some discussion was made on the findings mentioned above.
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  • Kosaku FUKAYA, Tomoo INOMATA, Masao ASARI, Yasunobu EGUCHI, Yutaka KAN ...
    1979 Volume 41 Issue 4 Pages 369-376
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    The thoracic course of the esophagus of bovine fetuses and neonates was examined by resin-casts of the thoracic hollow organs with a comparison of the formalin-hardened organs, with special reference to the site of constriction, curvature and ampulla. Resin and formalin injections were made into the esophagus, and trachea and the axillary artery and vein. The esophagus in the thorax had three constrictions. The first was located at the site pressed by the dorso-caudal end of the developing thymus and by the anterior border of the left anterior pulmonary lobe. The second was located at the site where the aortic arch was attached on its left side, just before the bifurcation of the trachea. The third was located very closely to the hiatus esophageus of the diaphragm, surrounded by the left and right posterior and the accessory pulmonary lobes and the muscular diaphragmatic crura which were well-developed characteristically in this species. The esophagus in the thorax had three curvatures. The first was concave ventrally, located between the level of the seventh cervical vertebra and that of the second thoracic vertebra. The second curvature was convex dorsally, beginning at the site of the attachment of the aorta to bend slightly ventro-caudad. The third was horizontal, beginning shortly behind the bifurcation of the trachea in the median plane of the body axis to bend gently toward the left-biased hiatus esophageus. The esophagus had an ampulla in the way of passing the posterior mediastenum.
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  • Masaaki TAMURA, Kunitoshi SUGIURA
    1979 Volume 41 Issue 4 Pages 377-383
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    To study the metabolism of vitamin D3 in dairy cows, the serum levels of vitamin D3 metabolites were determined in healthy dairy cows raised on normal ration. Newborn, young and adult cows and vitamin D3 administrated adult cows were used in the experiment. Serum levels of 1α, 25-dihydroxyvitamin D3 and 24(R), 25-dihydroxyvitamin D3 in the newborn calves were higher compared with those in young and adult cows, whereas serum 25-hydroxyvitamin D3 levels was not changed in those three groups. On the other hand, daily administration of 10, 000 IU vitamin D3 to adult cows resulted in the increases of serum 25-hydroxyvitamin D3 and 24(R), 25-dihydroxyvitamin D3 levels. However, serum 1α, 25-dihydroxyvitamin D3 levels in those cows were not different from those in adult cows which did not receive vitamin D3. Whereas single administration of 150, 000 IU vitamin D3 showed a slight increase in serum 25-hydroxyvitamin D3 and 24(R), 25-dihydroxyvitamin D3, and a significant increase in serum 1α, 25-dihydroxyvitamin D3. Correlation between serum vitamin D3 metabolites and serum Ca, P or Mg concentration was not observed. However, correlation was observed between 24(R), 25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3. Serum 1α, 25-dihydroxyvitamin D3 was maintained relatively constant level, except for newborn calves and adult cows treated with 150, 000 IU vitamin D3. However, serum levels of 25-hydroxyvitamin D3 and 24(R), 25-dihydroxyvitamin D3 vatied in a certain degree directly proportional to intake of vitamin D3.
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  • Kozo FUJISAKI, Katsuhiro MIURA, Haruhisa TAKAMATSU, Shigeo KITAOKA, Ts ...
    1979 Volume 41 Issue 4 Pages 385-390
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
    The present work was undertaken to observe the presence of auto-cold hemagglutinin in the plasma of chickens infected with Leucocytozoon caulleryi, and elucidate the immunological characteristics of this hemagglutinin. As a result, auto-cold hemagglutinin was found in all chickens infected with L. caulleryi. Pattern of the production and disappearance of this hemagglutinin was classified into two types, e.g., one peak type and two peaks type. It was noticed that the period of appearance of the highest titer of cold hemagglutinin coincided with that of anemia of chickens. Immunological characteristics of cold hemagglutinin was examined by the direct antiglobulin, the agar gel precipitation, and the absorption tests. The results of these tests suggested that the cold hemagglutinin in chickens infected with L. caulleryi was more likely, autoantibody rather than a parasite product, unlike the study by Kocan on Leucocytozoon simondi.
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  • Shinji YAMADA, Kazuo MATSUO, Terutoshi FUKUDA
    1979 Volume 41 Issue 4 Pages 391-393
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
  • Masayoshi NIIYAMA, Masashi OHBAYASHI
    1979 Volume 41 Issue 4 Pages 395-397,399
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
  • Yoshikazu IRITANI, Masayasu MIYAJIMA
    1979 Volume 41 Issue 4 Pages 401-403
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
  • Akira SHIMIZU, Eiichi KATO
    1979 Volume 41 Issue 4 Pages 405-408
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
  • Akira SHIMIZU, Eiichi KATO
    1979 Volume 41 Issue 4 Pages 409-412
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
  • Satoshi FUKUDA, Osamu MATSUOKA
    1979 Volume 41 Issue 4 Pages 413-415
    Published: August 25, 1979
    Released on J-STAGE: February 13, 2008
    JOURNAL FREE ACCESS
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