The Japanese Journal of Veterinary Science
Online ISSN : 1881-1442
Print ISSN : 0021-5295
ISSN-L : 0021-5295
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Volume 45, Issue 3
Displaying 1-23 of 23 articles from this issue
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  • Experimental Studies on Lymphography in Dogs : Clinical and Hematological Findings Following Lymphography of Pelvic Limbs
    Yoshikazu NISHIKAWA, Fumio TACHIBANA, Tadaaki KUDO, Kanjuro OTOMO, Tos ...
    1983 Volume 45 Issue 3 Pages 281-287
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.281
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    The present study was undertaken to obtain information on clinical and hematological influences of lymphography with Lipiodol Ultra-Fluide (LUF) in dogs. Experiments were carried out with 24 healthy adult dogs. They were divided into four groups: (1) intralymphatic injection of 0.4 ml/kg of Ringer's solution; (2) intralymphatic injection of 0.4ml/kg of LUF; (3) intravenous injection of 0.4 ml/kg of LUF; (4) intravenous injection of 1.2ml/kg of LUF. In group 1, no clinical or hematological changes were observed after the injection. In group 2, delayed wound healing, coughing, and a rise in the erythrocyte sedimentation rate were observed. In two dogs, arborizing vascular patterns appeared on their chest roentgenograms. In group 3, coughing was observed after the injection. Fine granular densities due to the droplets of the contrast medium appeared on their chest roentgenograms. There were no hematological changes. In group 4, such severe clinical signs as subnormal body temperature, decreased blood pH, and increased in plasma glucose were observed after the injection. The number of leukocytes also changed showing as a stress pattern. Two dogs died in 8 hr, and an obvious decrease in plasma calcium was observed in another dog. It is concluded that lymphography in dogs by intralymphatic injection of 0.4 ml/kg of LUF is not so hazardous, but as complications may sometimes occur, it should not be carried out without full knowledge of the procedure.
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  • Distribution of the a-Type Ganglion Cells in the Chicken Retina
    Toshihiko UESHIMA, Masato UEHARA
    1983 Volume 45 Issue 3 Pages 289-295
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.289
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    The distribution of retinal ganglion cells, particularly the α-type ganglion cells, in the chicken retinae was studied with whole mount preparations stained with cresyl violet. The density maps of all the cells including the glial cells and the displaced amacrine cells in the retinal ganglion cell layer showed that the area centralis was the region of the highest cell density. There was a drop in density about the circumference of the area centralis in all directions without the formation of the visual streak. However, the decrease in cell density was not so remarkable toward the superior-temporal region. The α-type ganglion cells in the area centralis were less numerous than those in the peripheral regions. The density of the α-type ganglion cells was higher in the inferior-temporal, inferior, inferior-nasal, nasal and superior-nasal fegions compared with that in the other regions. The distribution of the α-type ganglion cells in the chicken retina differed considerably from that in mammals.
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  • Pathology of Sialodacryoadenitis Virus Infection in Newborn Mice
    Akihiro KOJIMA, Hiroshi TAKADA, Hisashi YAMANAKA, Azusa OKANIWA, Kunio ...
    1983 Volume 45 Issue 3 Pages 297-304
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.297
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    Following intracerebral or intranasal inoculation with sialodacryoadenitis(SDA) virus, 930-10 strain, 2-day-old ddY mice developed central nervous system symptoms. The course of disease was peracute and pathological changes were exclusively seen in the brain, and they were localized symmetrically in the limited area of the cerebral cortex irrespective of route of inoculation. The lesions were characterized by degeneration of neurons without inflammatory changes. Multiplication of virions showing characteristics of coronavirus was detected in the degenerated neurons of the cerebrum. The virus existed also in the lungs of mice after intranasal inoculation. Virus specific immunofluorescence was observed in the areas of nerve cell layer of Stratum pyramidale subiculi and Cortex cerebri, area entorhinalis of the cerebrum.
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  • Pathology of Two Cases of Canine Disseminated Hypereosinophilic Disease
    Naoki GOTO, Masamichi KAWAMURA, Makoto INOUE, Akio SATO
    1983 Volume 45 Issue 3 Pages 305-312
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.305
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    Two spontaneous cases of canine disseminated hypereosinophilic disease were experienced. Main clinical signs were weight loss, severe dyspnea and coughing with abnormally high level of IgG, and in one case (Case A), bloody diarrhea occurred shortly before death. At necropsy, fibrous neoplastic nodules about 4 cm in diameter were produced around the trachea in both cases. Similar nodular lesions as well as some diffuse ones, were disseminated in the lungs, liver and spleen. In addition, the skin and gastrointestinal tract were severely affected in case A, while the kidneys and lymphoid tissues were involved in Case B. Histopathologically, all the lesions consisted of proliferated and sometimes degenerated fibrous tissues with dense accumulation of eosinophil leukocytes and plasma cells. By electron micro-scopy some eosinophils were shown to have specific granules of normal shape showing a degranulation process. The findings suggested an immunologic mechanism involved in pathogenesis of the disease.
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  • Intercellular Junction of Urodeal and Phallic Epithelial Cells in the Guinea Fowl, Numida meleagris
    Hiroyuki SASAKI, Takao NISHIDA, Koshi MOCHIZUKI
    1983 Volume 45 Issue 3 Pages 313-321
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.313
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    The intercellular junction was observed in urodeal and Phallic epithelial cells of the guinea fowl, Numida meleagris, by the ultra-thin section and the freeze-fracture methods to clarify the pathway of a lymph-like fluid produced by capillaries. Only desmosomes were found between cells of the stratified squamous epithelium covering the tips of paired lateral phallic bodies. The proximal portion of the lateral phallic body and the urodeum were lined with stratified and pseudostratified columnar epithelia containing numerous mucin-secreting cells. The columnar epithelial cells were linked with junctional complexes and cytoplasmic interdigitations. Half-desmosomes connected cells of the epithelial basal stratum with the basement membrane. The zonula occludens was well developed among mucin-secreting cells, but underdeveloped among columnar epithelial cells. When these junctions were examined in freeze-fracture replicas, the zonula occludens of the mucin-secreting cell exhibited a very distinct network of branching and joining strands. This type of tight junctions appeared to serve as a barrier to prevent lymph from diffusing by the intercellular route. On the other hand, the underdeveloped zonula occludens of the columnar epithelial cell was of incomplete reticular pattern in membrane fusion. It was suggested that the lymph produced by blood and lymph capillaries might be able to pass through the epithelium to play a part in dilution of the ejaculated semen.
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  • Circulating Antigens and Antibodies of Dirofilaria immitis in the Dog Detected by Counterimmunoelectrophoresis
    Masahiro TAGAWA, Kazuyoshi UEMATSU, Kazuo KUROKAWA, Hiroshi TANAKA
    1983 Volume 45 Issue 3 Pages 323-329
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.323
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    An experiment was carried out to clarify the rise and fall of soluble circulating antigen (SCA) in relation to the fluctuation of antibody levels in dogs experimentally infected with Dirofilaria immitis. For detection of SCA, counterimmunoelectrophoresis (CIEP) was performed continually in these dogs at given intervals. Besides CIEP, the double diffusion technique (DD) was used for examining the antibody level. When CIEP was conduced on the serum of dogs experimentally infected with D. immitis, SCA was first detected as early as 1 month after infection, and either SCA or antibody in the blood showed a rise and fall with the lapse of time, but antigen and/or antibody were always detectable in the course of infection. On the other hand, antibody appeared inconstantly in each infected dog in the course of infection when examined by DD. The results indicated that a high level of SCA was present in the circulating blood of dogs infected with D. immitis. As a result, CIEP was regarded as a highly reliable and useful diagnostic tool for examining infection with D. immitis, since it could detect both SCA and antibody simultaneously and the procedure was completed in a shorter time than DD or immunoelectrophoresis.
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  • Effects of Crowding and Heat Stress on Intestinal Flora, Body Weight Gain, and Feed Efficiency of Growing Rats and Chicks
    Kunio SUZUKI, Ryo HARASAWA, Yutaka YOSHITAKE, Tomotari MITSUOKA
    1983 Volume 45 Issue 3 Pages 331-338
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.331
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    The effects of crowding and heat stress on the intestinal flora, body-weight gains and feed efficiencies of rats and chicks were investigated. For the condition of crowding 25 rats were kept in a cage and 3 rats were kept in a cage for the control group. For the condition of heat stress, rats were kept at 31°C and 25°C for the control. The chicks were kept at 35°C for the heat stress and 25°C for the control. Irrespective of the types of stress and host species, aerobic bacteria, i.e., staphylococci and one or two bacterial groups of streptococci, enterobacteria and corynebacteria increased commonly in the small intestine. Sometimes anaerobes, i.e., peptococcaceae and bacteroides in rats increased under crowding condition and peptococcaceae and clostridia in chicks increased under heat stress. The changes of flora in the large intestine showed the similar tendency as in the small intestine. Body-weight gains and feed efficiencies were markedly suppressed under these stress conditions. These results showed that the changes of the intestinal flora and growth of host were affected with environmental stress.
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  • Electron Microscopic Findings of Experimental Atheromatous Lesions in Rats
    Kunio DOI, Akira YASOSHIMA, Kazuyuki KITAMURA, Hitoshi OKAWA, Azusa OK ...
    1983 Volume 45 Issue 3 Pages 339-346
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.339
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    Electron microscopic observation was carried out on the abdominal aorta and coronary artery of rats fed an atherogenic diet (containing 3% cholesterol, 1% cholic acid, 0.3% thiouracil and 21% hydrogenated fat) for 28 days combined with oral administration of vitamin D2 (320000 IU/kg/day) for the initial 4 days. Although the changes found in these two different types of artery were essentially similar to each other and characterized by the presence of lipid containing foam cells especially in the intima, some differences were detected between these arteries. Namely, deposition of fine fibrillar and granular materials, appearance of extracellular lipid crystals and increase in number of modified smooth muscle cells were apparent in the coronary artery, while increase in number of lipid-laden smooth muscle cells was conspicuous in the abdominal aorta. In addition, the present study demonstrated the possibility of transformation of both the smooth muscle cell and monocyte into foam cell.
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  • Studies on Enzyme-linked Immunosorbent Assay (ELISA) for Detection of Antibodies in Cattle Infected with Bovine Leukemia Virus
    Osamu KAJIKAWA, Hiroyuki KOYAMA, Takushi SASAKI, Takashi YOSHIKAWA, Hi ...
    1983 Volume 45 Issue 3 Pages 347-353
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.347
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    Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to bovine leukemia virus (BLV) was studied. In titration of anti-BLV antibody by ELISA, the glycoprotein (gp) antigen, the core protein (p) antigen, and the dual antigen containing both the gp and p antigens were used. A high titer of anti-BLV antibody was obtained by ELISA with the p antigen. A total of 114 cattle Sera were examined for anti-BLV antibody by ELISA with p antigen and by agar gel immunodiffusion test (AGIDT) with gp antigen. The ELISA and AGIDT titers agreed in about 93% of the specimens. In comparison between ELISA and AGIDT in detection of anti-p antibody with p antigen with these 114 bovine sera, ELISA showed a higher sensitivity in detection of anti-p antigen and higher antibody titers than AGIDT.
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  • Detection of Staphylococcus aureus in Bovine Mastitis and Some Characteristics with Special Reference to Enterotoxin Producibility and Coagulase Types of Isolates
    Kuniko TAKESHIGE, Kenya WATANABE, Hideo IGARASHI, Masao SHINGAKI, Take ...
    1983 Volume 45 Issue 3 Pages 355-362
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.355
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    During the period from november, 1979 to August, 1980, 332 samples of mastitis udders of cattle killed at the Tokyo Shibaura Slaughter House were investigated for the presence of Staphylococcus aureus. The isolates were tested for biochemical characteristics, enterotoxin production and coagulase typing. Eighty-seven S. aureus were isolated from the 85(25.6%) samples of 332 mastitis udders examined. Twenty-nine(33.3%) of the 87 strains produced enterotoxins A, B or C, and 24(82.8%) of them produced enterotoxin C. All except one strain, fell into either of types I through VIII in coagulase typing. Types VI(34.5%) and V(20.7%) were dominant among the eight coagulase types. Of the 87 strains 49(56.3%) coagulated bovine plasma irrespective of 100% in rabbit plasma coagulation. Twenty-nine(33.3%) isolates produced fibrinolysin, 82(94.3%) fermented mannitol, 40(46.0%) were positive in egg-yolk reaction, and all the strains grew on blood agar containing 1:200, 000 crystal violet showing the A type colonial character. Some of the staphylococal strains of bovine origin were negative in bovine plasma coagulation test, but positive in fibrinolysin. It is suggested from these results that S. aureus of bovine origin might be one of the causative agents for food poisoning.
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  • Physical Characterization of Non-Conjugative Citrate Utilization Plasmids
    Naotaka ISHIGURO, Katsumi KUBO, Gihei SATO
    1983 Volume 45 Issue 3 Pages 363-372
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.363
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    Seven citrate-utilizing (Cit+) Escherichia coli strains originated from horeses (2strains) and cattle (5 strains) were examined for transferability of Cit+ character and the presence of bacterial plasmids. All 7 strains harboured plasmids in the cell, although the Cit+ determinants in the Cit+ E. coli strains tested could not be transferred to E. coli K-12 strain. Two horse strains possessed about 60 megadaltons plasmids; the plasmid in a strain OH3026 was thought to be non-conjugative Cit plasmid, but that in OH3025 was not exactly shown to encode the Cit+ functional ability. Plasmid DNAs prepared from 2 strains (OH3035 and OH3036) of 5 bovine strains could produce Cit+ transformants, but no Cit+ transformant was obtained in the remaining 3 strains. The representative non-conjugative Cit plasmid, pOH3035 (20.5kb), prepared from the Cit+ transformant of pOH3035 was examined by restriction endonuclease, and its EcoRI-cleaved B fragment (5.5kb) carrying Cit+ functional gene was cloned to a vector plasmid pBR322. The Cit+ recombinant plasmid, pOH8 constructed by cloning was analysed for potential transposability of Cit+ gene, but the transposable element coding for Cit+ determinant was not obtained from pOH3035 and pOH8 in this study. The restriction map of pOH3035 was also compared with that of a plasmid pBR322 carrying citrate utilization transposon, pOH2, and the relative similar order of recognition sites for BamHI and BglII enzymes was found on Cit+ functional gene of the 2 plasmids.
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  • A Cytochemical Study on Feline Blood Cells
    Hajime TSUJIMOTO, Atsuhiko HASEGAWA, Isamu TOMODA
    1983 Volume 45 Issue 3 Pages 373-382
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.373
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    Cytochemical characteristics of the feline blood cells in peripheral blood and bone marrow were examined with various staining methods. The neutrophilic cells showed the distinct positive reactions of peroxidase, naphthol-AS-D-chloroacetate esterase, phosphorylase, Sudan black B and periodic acid Schiff. The eosinophilic cells exhibited a marked acid phosphatase activity. The monocytes disclosed the characteristic positive reaction in the esterase staining using alpha-naphthyl acetate, alpha-naphthyl butyrate or naphthol-AS-acetate as a substrate. About the half of the lymphocytes exhibited the granularly positive reactions in acid phosphatase, esterase and beta-glucuronidase stainings. Of these cytochemical reactions, the positive reactions of the neutrophilic cells in phosphorylase and periodic acid Schiff stainings gradually increased in intensity according to their maturation. On the contrary, the immature neutrophils reacted more intensely than the mature ones in peroxidase, naphthol-AS-D-chloroacetate esterase and Sudan black B stainings. Similarly, the activities of acid phosphatase, beta-glucuronidase and dehydrogenases in the neutrophilic, erythrocytic and megakaryocytic series gradually decreased in intensity according to their maturation. These cytochemical properties of the feline blood cells were considered to be the valuable markers indicating the blood cell line and the degree of maturation.
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  • Polycystic Immature Teratoma Observed at the Head of Meckel's Diverticulum of a Chick
    Masahiro SUGIYAMA, Masae ISODA, Atsushi TOMIZAWA
    1983 Volume 45 Issue 3 Pages 383-385
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.383
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  • Cytochemical Identification of PAS Positive Granules in Eimeria tenella Sporozoite by Microspectrophotometry
    Yutaka NAKAI, Keiji OGIMOTO
    1983 Volume 45 Issue 3 Pages 387-389
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.387
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  • Spontaneous Endocardial Schwannoma in a Rat
    Midori TANAKA, Kunitoshi MITSUMORI, Keizo MAITA, Yasuhiko SHIRASU
    1983 Volume 45 Issue 3 Pages 391-394
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.391
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  • A Case of Feline Macroglobulinemia
    Takatsugu YAMADA, Akie OGURA, Jun INOUE, Hajime TSUJIMOTO, Kenichiro O ...
    1983 Volume 45 Issue 3 Pages 395-399
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.395
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  • Ultradian Rhythm of Chicken Body Temperature under Continuous Light
    Hiroshi KADONO, Eiichi USAMI
    1983 Volume 45 Issue 3 Pages 401-405
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.401
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  • Incidence of Lymphocytic Nuclear Pockets in Bovine Leukosis Observed in Hokkaido
    Duck-Hwan KIM, Shigeru ICHIJO, Tatsuo KONISHI
    1983 Volume 45 Issue 3 Pages 407-412
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.407
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  • Motor Nerve Conduction Velocity of the Ulnar and Tibial Nerves and Characteristics of M Wave of the Interosseous Muscles in the Adult Dog
    Yuko TAKAKURA, Shichiro INADA
    1983 Volume 45 Issue 3 Pages 413-416
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.413
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  • Isolation and Characterization of Non-flagellated and Paralyzed Flagellated Mutants of Clostridium chauvoei
    Yutaka TAMURA, Nobuyuki MINAMOTO, Shozo TANAKA
    1983 Volume 45 Issue 3 Pages 417-420
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.417
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  • Electron Microscopic Findings of Vitamin D2-Induced Glomerular Lesions in Rats
    Kunio DOI, Akira YASOSHIMA, Hitoshi OKAWA, Azusa OKANIWA
    1983 Volume 45 Issue 3 Pages 421-424
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.421
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  • Direct Perfusion Fixation of Cochlear Organ : A Practical Method for Histopathological Examination
    Isao WADA, Yoshio MINATO, Masakazu TAKESHITA, Takashi KOBAYASHI, Azusa ...
    1983 Volume 45 Issue 3 Pages 425-430
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.425
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  • Transition of Precipitating Antibodies against Antigens from Adult Flukes and Metacercariae of Fasciola sp. in the Infected Rabbits
    Shinobu YOSHIHARA, Kyo SUZUKI
    1983 Volume 45 Issue 3 Pages 431-434
    Published: June 05, 1983
    Released on J-STAGE: February 13, 2008
    DOIhttps://doi.org/10.1292/jvms1939.45.431
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