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Volume 41 (1979)
- Issue 6 Pages 583-
- Issue 5 Pages 471-
- Issue 4 Pages 351-
- Issue 3 Pages 227-
- Issue 2 Pages 97-
- Issue 1 Pages 1-
Volume 41, Issue 5
Displaying 1-12 of 12 articles from this issue
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Noboru KUBA, Kohei MIYAWAKI, Fuminori FUNABASHI, Masanori KOBAYASHI, S ...1979 Volume 41 Issue 5 Pages 471-481,485
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSTwo experiments, I and II, were carried out with 15 piglets (hybrids of Landrace × Yorkshire) approximately 60 days old. Six piglets were used for experiment I and the other nine for experiment II. The former were fed a high lysine diet, and the latter a low lysine diet for 4 months. DL-methionine and P/Ca ratio were formulated to meet the levels recommended by the National Research Council, U.S.A., in 1973. The nutrient contents and amino acids of the diet were made as much the same as possible for the two control groups of experiments I and II. The 9th right costochondral joint was selected in all the animals for the present study, because it exhibited more characteristic lesions than any other site examined. The results obtained are summarized as follows. (1) Growing piglets fed a high lysine diet revealed, as a whole, relatively mild disturbances in the ossification process of the costochondral joint. (2) Growing piglets fed a low lysine diet revealed, as a whole, marked disturbances in the ossification process of the costochondral joint.View full abstractDownload PDF (2075K) -
Tsuyoshi ISHIMINE, Hideyuki NAGASAWA, Naoyoshi SUZUKI1979 Volume 41 Issue 5 Pages 487-493
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe monocyte-macrophage cell line is an important marker of the host defense system especially in cell-mediated immunity. This report describes a series of assays that can be applied routinely in canine monocyte function, or phagocytosis and gives some information as to the activation of normal monocytes by sensitized lymphocytes in dogs infected with Babesia gibsoni. Babesia-immune monocytes were more effective in phagocytosis of the parasitized erythrocytes than normal monocytes. Babesia-immune monocytes incubated with Babesia-immune fresh serum showed the highest percentage of phagocytosis. When normal monocytes were incubated for 24 hours with Babesia-immune lymphokines, the percentage of phagocytic monocytes was approximately three times higher than those with normal cultured supernatant.View full abstractDownload PDF (792K) -
Hirokazu TSUBONE, Hiroshi SAWAZAKI1979 Volume 41 Issue 5 Pages 495-504
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe electroencephalographic (EEG) activity was studied in ICR mice. EEG patterns obtained by bipolar leads from the mice were classified into five stages: awake-exciting (AE), awake-resting (AR), drowsy (DR), deep sleeping (DS) and REM sleeping (RS) stage. These stages were characterized by the following EEG patterns. AE: Rhythmic θ-activity of 4 to 6 Hz accompanied with an irregular low voltage and fast waves appeared continuously. AR: High voltage and slow waves (HVSWs) lasting for a few seconds appeared at intervals of 3 to 10 seconds. DR: Spindles with a duration of 1 to 5 seconds were frequently observed at intervals of 3 to 5 seconds. DS: Bursts of HVSWs and spindles were reciprocally repeated with durations of 5 to 10 and 10 to 30 seconds, respectively. RS: The θ-activity of 6 to 8 Hz lasting for 2 to 250 seconds appeared with a regular rhythm. The five stages were interchanged within such a short time that the cyclic occurrence of the RS stage was shown at intervals of about 20 seconds to 10 minutes. From these results it was concluded that the electrical characteristic of the brain of the mouse was the conspicuous appearance of θ-activity and spindles, and that the EEG stages alternated frequently with one another in this animal.View full abstractDownload PDF (879K) -
Mitsuaki HAYASHI, Yu IDE, Shigemi SHOYA, Chikashi ENOMOTO, Harutoshi M ...1979 Volume 41 Issue 5 Pages 505-510
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSObservation was made on beef calves with xanthinuria and xanthine calculosis to describe clinical biochemical changes of these diseases. In these calves urea was markedly high and uric acid low in serum level. The urine was acid in reaction and negative for all the routine qualitative tests. Xanthine was remarkably high and uric acid low in urine concentration. The Xanthine contents of calculi were 51.5 to 96.9% in dried matter. The infrared spectrophotometric spectra of the calculi were consistent with those of the authentic xanthine standard. The activities of xanthine oxidase in the liver, spleen, kidney and small intestine were below 1% of those in the respective organs from healthy cattle.View full abstractDownload PDF (834K) -
Shotaro TAKEUCHI, Ryozo AZUMA, Tsuneji SUTO1979 Volume 41 Issue 5 Pages 511-516
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSHemolysin was purified from the culture supernatant of Corynebacterium pyogenes by successive ultrafiltration with PM 10 membrane, column chromatography on carboxymethyl cellulose and Sephadex G-200. The purified hemolysin was a substance of high molecular weight. This hemolysin formed only one band in polyacrylamide gel electrophoresis, but it gave about five bands in SDS-polyacrylamide gel electrophoresis. The hemolytic activity was not enhanced by the addition of L-cysteine, sodium hydrosulfite or sodium thioglycolate. Most of heavy metals did not inhibit nor stimulate the hemolytic activity. The hemolytic activity was completely destroyed by pronase or trypsin, but not by pepsin. It was inactivated by heating at 60°C for 10 minutes. Rabbit, horse and pig erythrocytes were most sensitive to the hemolysin and chicken erythrocytes were insensitive. The LD50 of hemolysin was approximately 96 hemolytic units per mouse.View full abstractDownload PDF (619K) -
Chitoshi ITAKURA, Masato TAKECHI, Yoshifumi MIYAKAWA, Masatomo GOTO1979 Volume 41 Issue 5 Pages 517-530
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSBones and parathyroid glands of 48 broiler chickens from 28 to 69 days of age, naturally affected with rickets, were pathologically studied. Main gross lesions were stunting of the skeletal growth, enlargement at the caput costae, hypertrophy of all the bones and enlargement of the parathyroid glands. Histopathologic lesions of the bones were roughly divided into three stages taking into consideration the developmental process. Stage I of the lesions was the initial alteration consisting of mild widening of the hypertrophic zone. In stage II there were marked widening of the hypertrophic zone with considerable endochondral ossification, a moderate degree of endosteal osteogenesis with osteoclastic resorptions in the marrow cavity, and frequent periosteal osteogenesis and demineralization in the cortex. In stage III most portions of the hypertrophic zone were replaced by osteoid trabeculae and the proliferating zone was widened. Endosteal osteogenesis was conspicuous and extensive areas of the marrow cavity were occupied with abnormal osteogenous tissue. All the parathyroid glands examined showed histopathologic features of hyperparathyroidism consisting of swelling and hyperplasia of chief cells. It was concluded that the bone lesions in rickets of growing broiler chickens varied with time and there was a close relationship between formation of the bone lesions and hyper-parathyroidism. Etiologically, this condition might be due to vitamin D3 deficiency in the feed.View full abstractDownload PDF (3165K) -
Akio SHIGA, Ken-ichi SHINOZAKI1979 Volume 41 Issue 5 Pages 531-540
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSAn experiment was conducted on the balance between Mg and Ca. In it 4 ewes were fed a control diet for 10 days, a low Mg and low Ca diet for the following 10 days (Experiment I), and a low Mg diet supplemented with CaCO3 for the following 8 days (Experiment II). 0n Exp. I, the decrease in dietary Mg and Ca resulted in a significant decrease in the fecal and urinary excretion and a significant fall of serum concentration of Mg and Ca. A significant positive correlation between Mg and Ca was the most remarkable in an old ewe (8 years old) of the four used. In the first half part of Exp. II, the increase in dietary Ca resulted in a significant increase of apparent absorption of Ca, and a temporary increase and the following decrease of urinary and serum Ca in all the ewes. In an old ewe the fecal and urinary excretion of Mg increased and hypomagnesemic tetany occurred with a remarkable fall in serum Mg level (0.29 mg/dl) and a rise in serum Ca/Mg ratio (24.7) on the fourth day of experiment. In the other three ewes, the balance of Mg did not change, but the serum Mg level fell to 1.15 mg/dl on the fourth day. In the second half part of Exp. II, the old ewe could not stand up to have feed and water, and died of tetany on the seventh day. The remaining three ewes exhibited remarkable inappetence on the fifth and sixth day, but gradually recovered on and after the seventh day.View full abstractDownload PDF (962K) -
Muneo NAKAZAWA, Hisashi NEMOTO1979 Volume 41 Issue 5 Pages 541-543
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Kaoru KOSHIMIZU, Teruo MAGARIBUCHI, Masahiro ITO1979 Volume 41 Issue 5 Pages 545-549
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Shinji YAMADA, Kazuo MATSUO, Terutoshi FUKUDA1979 Volume 41 Issue 5 Pages 551-553
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Katsuhiro FUKUTA, Takao NISHIDA, Koshi MOCHIZUKI1979 Volume 41 Issue 5 Pages 555-559
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Chitoshi ITAKURA, Kikuyasu NAKAMURA, Junko NAKATSUKA, Masatomo GOTO1979 Volume 41 Issue 5 Pages 561-566
Published: October 25, 1979
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS
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