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Volume 43, Issue 1
Displaying 1-13 of 13 articles from this issue
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Katsuya ISHIHARA, Hitoshi KITAGAWA, Shinji YOKOYAMA, Hidekazu OHASHI1981 Volume 43 Issue 1 Pages 1-11
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSChanges of free cholesterol (F Ch) and phospholipid (Pl) collcentrations in the serum and the contents in the red cell membranes were investigated in the case of dirofilarial hemoglobinuria (Hb-uria) and compared with those in normal dogs and dogs suffering from chronic serious filariasis without Hb-emia and Hb-uria. There was a significant increase in serum F Ch concentration and in the Ch and Pl contents in the red cell membranes of the serious filariasis group, as compared with the normal one. Marked increases in the F Ch and Pl concentrations and F Ch/Pl ratio were found in the serum, the Ch content and Ch/Pl ratio in the red cell membranes in the Hb-uria group, as compared with the normal one. Also, as opposed to the serious filariasis group, serum F Ch concentration showed an appreciable rise, but the Ch/Pl ratio was insignificant in the red cell membranes. There were no significant findings as to the serum lipoprotein fractions in the Hb-uria or the normal group. In the Hb-uria group, however, the α-lipoprotein concentration and α/total β-ratio were significantly higher and the total β-lipoprotein concentration was significantly lower in the serum than in the serious filariasis group. There were significant correlations between the serum and red cell membranes as to F Ch concentration and Ch content, or Ch/Pl ratio, in the normal and whole groups, but not in the Hb-uria group. Both filariasis groups showed an appreciable increase in erythrocyte mechanical fragility, as compared with the normal group, and they displayed a lower minimum and higher maximum resistance to osmotic fragility than the normal group.View full abstractDownload PDF (1092K) -
Kiheiji SHIMIZU, Shun-ichiro MASAKI, Tsuneo HIROSE1981 Volume 43 Issue 1 Pages 13-19
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSBacteriological studies were carried out on the prevalence of mycobacteria among cows. The results obtained are as follows. Nine cows sacrificed as infected with tuberculosis were examined. Nodular thelitis was detected from three of them and mycobacteria were demonstrated microscopically in six, but Mycobacterium gordonae-like organisms were isolated from only one. Until now, nodular thelitis was reported in 19 cases, including the present 12 cases. In 7 cases, the cows were slaughtered as infected with tuberculosis. The tuberculin test was conducted experimentally on other 4 cows with nodular thelitis, all of which reacted positive to the mammalian type and two of which to the avian type. Mycobacteria were microscopically demonstrated in lesions of the 4 cows. Isolation of mycobacteria, however, was successful from only one of those 12 cases. The isolate was identified as an M. vaccae-like organism. An attempt was made to isolate mycobacteria from the lymph nodes of 140 apparently healthy cows at the abattoir. Rates of isolation were as follows: 3.05% (4/131) from the mesenteric, 1.54% (2/130) from the submaxillary, and 0.74% (1/135) from the pulmonary lymph nodes. Total rate of isolation reached 5.0%. The isolates were identified as follows: three as M. fortuitum, one as an unknown rapid grower, two as M. scrofulaceum, and one as M. intracellulare serovar. IIIb. M. tuberculosis was not demonstrated. As main causes of the no-lesion reactors in Hokkaido, nodular thelitis and the sensitization induced by atypical mycobacteria must be taken into consideration hereafter.View full abstractDownload PDF (696K) -
Mineo HAYASAKI1981 Volume 43 Issue 1 Pages 21-26
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSFour kinds of Dirofilaria immitis antigens, prepared from intrauterine micro-filariae (I-Mf), circulating microfilariae (C-Mf), migrating larvae and adult worms (A-Di), were assessed by indirect hemagglutination (IHA) test in terms of immunological specificity and sensitivity. The highest specificity and sensitivity were observed in phosphate buffered saline (PBS) extract of I-Mf among these antigens. In contrast, PBS extract of C-Mf was found to have extremely low IHA antigenicity. Cross reactions between D. immitis and some intestinal parasites, such as Toxocara canis, Ancylostoma caninum and Trichuris vulpis, were evaluated by agglutinin absorption assay. It was revealed that no substantial cross reaction was present between I-Mf or A-Di and the above three intestinal parasites. Agglutinin absorption assay also suggested that the antigenicity of A-Di antigen differed from that of I-Mf antigen, although some common antigens were present between them. The present results indicate that I-Mf antigen is useful for IHA test in D. immitis infection in dogs.View full abstractDownload PDF (732K) -
Yasunobu EGUCHI, Yonetaka FUKIISHI, Yoshio MORIKAWA1981 Volume 43 Issue 1 Pages 27-36
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThyroid C cells in fetal rats on days 21 and 22 of gestation had well-developed rough endoplasmic reticule (RER) and Golgi complexes and numerous secretory granules. The granules had vascular polarity in their location and some of them were attached to the cell membranes. Fetal hypophyseoprivia by subtotal decapitation (SD) caused fetal hypercalcemia, accompanied by a wide variation in electron density of the secretory granules, an increase in number of flattened RER and a further development of Golgi complexes. These changes in fine structure suggest a rise in calcitonin-producing activity of C cells, probably in response to hypercalcemia. Fetal thyroidectomy caused fetal hypocalcemia, probably owing to parathormone deficiency. TSH therapy of SD fetuses neither prevented the fetal hypercalcemia nor repaired the cytologic changes in C cells. GH therapy of SD fetuses partially prevented the hypercalcemia between day 20 and day 21 of gestation, but not between day 21 and day 22. Cytologic repair was not observed. Propylthiouracil injected into fetuses influenced neither the C cell's structure nor the plasma calcium levels. The observations suggest that the fetal hypercalcemia and the rise in secretory activity of C cells induced by fetal SD are not solely due to a deficiency of fetal pituitary TSH. The role of pituitary GH is questionable. Deficiencies of other brain factors should be considered as contributing to the observations.View full abstractDownload PDF (1792K) -
Yoshio KOJIMA1981 Volume 43 Issue 1 Pages 37-41
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSBy means of a transmission electron microscope, various types of intracellular vacuoles or vesicles were observed in ejaculated boar spermatozoa. These membrane bounded structures were frequently noticed in the head (acrosome and sperm nucleus) and the principal piece, but were hardly ever seen in the middle piece except in the cytoplasmic droplet, or in the end piece. In two cases of sterility (asthenozoospermia) and poor semen quality (hairpin curved deformity), the vacuoles appeared in the neck area, even in the center of proximal centriole, and some of them represented an invagination of the plasma membrane.View full abstractDownload PDF (692K) -
Manabu SAITO, Masaro NAKAGAWA, Eiko SUZUKI, Kuniaki KINOSHITA, Kiyoshi ...1981 Volume 43 Issue 1 Pages 43-50
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe synergistic effect of Sendai virus on Mycoplasma pulmonis infection was investigated in ddY mice infected with the two agents. As the results, growth of M. pulmonis was remarkably enhanced in the lung by superinfection of Sendai virus, although not significantly affected in the trachea. The enhanced growth of M. pulmonis was most evident when the both organisms infected at the same time, and the less enhancement of the growth was observed in the longer time difference between infections of the two agents. Detection rates of pneumonia in mice infected with both M. pulmonis and Sendai virus were 80 to 100 per cent, remarkably higher than 12 per cent of control mice infected with M. pulmonis alone, and comparable to 80 per cent of control mice infected with Sendai virus alone. However, the lesions in the dually infected mice were more severe and persisted longer than those in the mice infected with Sendai virus alone. CF antibody against M. pulmonis persisted longer in the dually infected mice, as compared with that in control mice infected with M. pulmonis alone. Production of HI antibody to Sendai virus was considerably inhibited by combined infection with M. pulmonis.View full abstractDownload PDF (790K) -
Yutaka TANAKA, Yasuji KATSUBE, Takeshi MUTOH, Kiyoshi IMAIZUMI1981 Volume 43 Issue 1 Pages 51-59,62
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSIn mice given orally 103 to 107 cells of a fimbriate descendant of S. typhimurium, the organism localized in between the middle to lowest parts of the small intestine and large intestine, especially in the walls 1 to 3 days postadministration. Thereafter, the organism multiplied in the tracts significantly, and with the increase of inoculating doses, remarkable multiplication of the organism in the tracts was shown. The recovery of the organism from the mesenteric lymph node, liver or spleen with or without gross lesions was found 3 to 5 days after administration, and the liver harbored 102 to 107/g organisms between 5 and 14 days. A mouse given 105 cells died of sepsis on the 6th day, and 7 mice received 107 cells within 6 to 13 days. The O, H and fimbrial antibodies were found in the mice from the 7th day. On the other hand, in mice given a non-fimbriate descendant, no evidence was shown to prove the colonization of the organism in the digestive tracts. Exceptionally, a few mice given 105 to 107 cells produced O and H antibodies against the organism. On the contrary, it was reconfirmed that mice inoculated intraperitoneally with fimbriate and non-fimbriate descendants gave almost the same LD50 value as reported previously. By scanning electron microscopy, fimbriate organisms were found to adhere to the epithelial surface of the villi (in vitro), while non-fimbriate organisms were not seen on the mucosal surface. These results suggest that the fimbriae may play a role in the colonization of Salmonella organisms in the intestinal mucosa.View full abstractDownload PDF (1563K) -
Makoto ENDO, Morikazu SHINAGAWA, Hitoshi GOTO, Kiheiji SHIMIZU1981 Volume 43 Issue 1 Pages 63-70
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe replication of feline panleukopenia virus (FPLV) in the synchronized kitten kidney (KK) cells was studied. The adsorption rates of [3H]-thymidine-labeled FPLV to each of the G1, early S and M phases of cell cycle were almost the same. The KK cells maintained in the G1 phase did not support the replication of FPLV through the 12 to 36 hours of the observation period. However, when the cell cycle was allowed to proceed by trypsinizing G1 phase cells at 0, 6 and 12 hours after virus infection, a slight replication of the virus was observed. A high replication of FPLV was obtained from the cells infected at early S phase. The early S phase cells arrested with 2mM thymidine (TdR) also supported well the growth of FPLV when the initiation of the cell cycle was performed by the removal of TdR within 12 hours after infection, early S phase arrested cells themselves did not. No replication of FPLV was observed in the M phase cells. These results indicate that FPLV requires the DNA synthesizing system of KK cells for its replication.View full abstractDownload PDF (864K) -
Bunei SYUTO, Yoichi MIYAKE, Shuichiro KUBO1981 Volume 43 Issue 1 Pages 71-77
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSAn improved method was devised for polyacrylamide gel electrophoresis by controlling the electrophoretic distance. It was applied to analysis of serum proteins. By it a high resolution and a good reproducibility were obtained on electrophoretic protein patterns on both single-layer and concentration-gradient gels. The variations of the electrophoretic distance of protein were within 0.6% in 7% plain gel and with 0.69% in gradient gel. There were from 65 to 81 protein zones detected in gradient gel which contained aspartic acid as a leading ion, instead of Cl-, in human serum. The improved method was applied to analysis of changes in protein pattern in the serum of newborn calf. The electrophoretogram showed that seven additional zones corresponding to the colostrum appeared in the serum within 24 hours after ingestion of the colostrum. It also revealed that transferrin changed in the number of components and in the concentration of protein. That number and this concentration must be related with the activation of erythropoiesis in the newborn calf. The improved method is suitable for the detailed analysis of serum proteins.View full abstractDownload PDF (810K) -
Kan-ichi OHSHIMA, Kosuke OKADA, Shigeru NUMAKUNAI, Yotaro YONEYAMA, Sh ...1981 Volume 43 Issue 1 Pages 79-81
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
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Shunsaku FUJII, Tatsudo TAMURA, Toshikazu OKAMOTO1981 Volume 43 Issue 1 Pages 83-85,88
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
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Muneo NAKAZAWA, Hisashi NEMOTO, Hisashi UEDA, Tsutomu MARUYAMA1981 Volume 43 Issue 1 Pages 89-91
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS -
Masamichi KUROHMARU, Takao NISHIDA, Koshi MOCHIZUKI, Yoshihiro HAYASHI1981 Volume 43 Issue 1 Pages 93-95,98
Published: February 25, 1981
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS
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