In the previous report it was stated that large numbers of cows having alcohol positive milk were affected by liver dysfunction. Accordingly, in this paper we go on to show how experimental liver dysfunction is brought about in goats in order to produce alcohol positive milk. As inhibitors of liver function, hexachlorethane, carbon tetrachloride, and allylformate, were used. The results obtained from this experiment may be summarized as follows. 1)When goats were administered orally, with 0.8g/kg of hexachlorethane, subcutaneously, with 0.1cc/kg of carbon tetrachloride and intraperitoneally, with 0, 01cc/kg of allyl formate, they, with the exceptiton of a few goats, secreted alcohol positive milk. 2)We carried out liver function tests by BSP and Gros reaction, the ascertaining of blood sugar components, serum protein fraction by electrophoretical apparatus, DNA, PNA, acid-soluble-P and lipid-P of liver homogenate, TTC reduction test and alkali-phosphatase activity test of liver homogenate, and by histological examination of the livers. As to the results, the experimental groups showed some abnormality in the BSP of liver function test when compared with the normal group. In electrophoretic analysis of serum protein, a lower level of albumin and a higher level of γ-globulin was observed, especially, and the ratio of albumin / globulin was also low in the experimental groups. The contents of DNA, PNA, acid-soluble-P and lipid-P were a little lower in the liver disordered groups than in the normal group. In the histological examination of livers, disappearance of nuclei, fatty degeneration, and slight congestion of liver cells, were found. 3)Changes of milk contents and milk quality. When the milk of goats with liver dysfunction showed positive reaction to alcohol reagent, the contents of total protein, total calcium, ionized calcium and total magnesium had a tendency to increase in general, then they decreased gradually, with the exception of the ionized calcium. Such contents as non-protein-nitrogen and amino-nitrogen showed a tendency to increase a little. On the other hand, casein of milk protein fraction, inorganic phosphorus, ultrafiltrated phosphorus, and citric acid, decreased. From these results, the cause of the increase of ionized calcium in milk is thought to be due to the decrease of casein, ultrafiltrated phosphorus, and citric acid, that combine with ionized calcium. The milk quality test in the case of the milk of experimental groups, showed no remarkable changes when compared with the control group, except for a few examples. From the results described above, it is thought that there is a possibility of producing alcohol positive milk by artificial liver dysfunction. It has already been reported in the previous paper that a large number of cows in the field cases of alcohol positive milk seemed to be in the state of liver dysfunction. From both facts described above, it is concluded that cows probably secret alcohol positive milk due to contracting liver dysfunction in some way or other.

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(1) Not only in tertiary amine compounds, but also in secondary and primary ones, the inactivating ability of nitrogen mustards on hog cholera virus was proven. (2) Destruction of biological activity of hog cholera virus appears not to be related to the chemical groups attached to the nitrogen atom of bis-β-chloroethyl amine. Therefore, significance of virus inactivation may not be attributed to benzyl group. (3) In a single experiment, the inactivation of hog cholera virus was more strongly influenced by monofunctional rather than bifunctional mustard compounds. (4) Triethylenemelamine inactivated hog cholera virus. (5) The basis of inconsistencies for inactivation and variance in immunogenicity of derivative hog cholera virus by nitrogen mustard compounds was discussed.

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