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Volume 40 (1978)
- Issue 6 Pages 633-
- Issue 5 Pages 515-
- Issue 4 Pages 367-
- Issue 3 Pages 265-
- Issue 2 Pages 121-
- Issue 1 Pages 1-
Volume 40, Issue 3
Displaying 1-13 of 13 articles from this issue
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Norinaga MIWA, Kensuke CHIKAMORI, Bunei SYUTO, Shuichiro KUBO1978 Volume 40 Issue 3 Pages 265-274
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSPorcine erythrocyte adenylate kinase showed three components, AKa, AKb and AKc, designated on the basis of the distance of migration from their origin to the anode in starch gel electrophoresis. By isoelectrofocusing, 96% of the total erythrocyte adenylate kinase focused at pH 9.25, and this predominant enzyme form corresponded to AKa in starch gel electrophoresis. The predominant enzyme form, AKa, was purified at approximately 31, 000 fold from the porcine erythrocyte in an overall yield of 45% by affinity chromatography on a column of Blue Dextran-Sepharose 4B, substrate elution from a column of phosphocellulose, and by isoelectrofocusing. The purified AKa had a specific activity of 2, 100 units per mg of enzyme and migrated in gel electrophoresis as a single band having a molecular weight of 21, 500. The amino acid composition of AKa was the same as that of the skeletal muscle enzyme. Peptide mapping showed a correspondence of all spots of the tryptic peptides from the erythrocyte and skeletal muscle enzyme preparations. These results strongly suggest that the predominant adenylate kinase in porcine erythrocyte is identical to porcine skeletal muscle adenylate kinase.View full abstractDownload PDF (1026K) -
Masamitsu KANOE, Yuji IZUCHI, Mitsuyoshi TODA1978 Volume 40 Issue 3 Pages 275-279,281
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSA survey was conducted on the occurrences of rumen parakeratosis, rumenitis, traumatic reticulitis, and reticular and hepatic abscesses among 492 slaughtered cattle in Yamaguchi and Fukuoka Prefectures, Japan. These diseases occurred to 59 animals (12.0%), 83 (16.9%), 26 (5.3%) and 25 (5.1%), respectively. Hepatic abscesses were detected from 18 (12.0%) of 149 animals with rumen parakeratosis and/or rumenitis, 3 (11.5%) of 26 with traumatic reticulitis, 1 (12.5%) of 8 with reticular abscesses, and 3 (1.0%) of 309 with no disorders. Fusobacterium necrophorum was detected from 9 of 20 cases of rumen parakeratosis, 12 of 20 cases of rumenitis, 1 of 10 cases of unaffected ruminal tissue, and all of the cases of reticular and hepatic abscesses. The viable count of F. necrophorum was 104 to 106 per gram in the cases of rumen parakeratosis, 103 to 107 in those of rumenitis, and 105 in that of unaffected ruminal tissue.View full abstractDownload PDF (705K) -
Manabu SAITO, Masaro NAKAGAWA, Kuniaki KINOSHITA, Kiyoshi IMAIZUMI1978 Volume 40 Issue 3 Pages 283-290
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSEtiological studies of pneumonia in mice were made on natural outbreaks, with special reference to P. pneumotropica, M. pulmonis, C. kutscheri and Sendai virus. Pulmonary consolidation and suppurative lesions with bronchiectasis were rarely detected in mice from either colonies free from all the organisms examined or those infected with P. pneumotropica and/or M. pulmonis. In colonies infected with Sendai virus, however, the lung lesions were demonstrated in 1.7 to 78.3% of mice, regardless of the presence or absence of other pathogens. Considerably high incidence of the pulmonary lesions, 7.6 to 20.0%, were observed in mice from all colonies infected with P. pneumotropica, M. pulmonis and Sendai virus. From the pulmonary lesions, M. Pulmonis was isolated at high frequencies and in a larger number, while some were detected from grossly normal lungs. With P. pneumotropica, the results were similar but the difference between the affected and normal lungs was less remarkable. Mice harboring both M. pulmonis and P. pneumotropica in their lungs were mostly positive for Sendai virus antibody. C. kutscheri was rarely isolated from pulmonary lesions in the present cases.View full abstractDownload PDF (834K) -
Nobuyuki TERAKADO, Hisashi UEDA, Hiroharu SUGAWARA, Yasuro ISAYAMA, No ...1978 Volume 40 Issue 3 Pages 291-295
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSBrucella canis strains isolated from house dogs, stray dogs and beagles in breeding or experimental colonies were examined for their susceptibility to 38 antibacterial drugs by an agar dilution method. All of 90 strains tested were found to be highly susceptible to tetracyclines and aminoglycoside antibiotics. These were also susceptible to chloramphenicol, spectinomycin, rifampicin and sulfonamide. However, in these isolates, decreased susceptibility was found to cephalosporin antibiotics and nalidixic acid, and almost insensitivity to peptide antibiotics and cycloserine. On the other hand, 22 (24.4%) of 90 strains tested were found to be multiply resistant to macrolide antibiotics, some of penicillins, novobiocin and lincomycin. These multiple drug-resistant strains were isolated from stray dogs and beagles in various districts, indicating that the prevalence of such resistant strains is relatively wide-spread in dogs in Japan.View full abstractDownload PDF (516K) -
Hideo NIGI1978 Volume 40 Issue 3 Pages 297-307
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSNine female Japanese monkeys were ovariectomized. The exact time of ovulation was determined in them by laparoscopic observation of ovaries. Accurately dated ovaries were examined histologically. Some slight folds and some large lutein-cell-like granulosa cells were seen in a pre-ovulatory follicle. They suggested that a luteinization might have started in an ovary before ovulation. The formation of a new corpus luteum progressed gradually during the first 40 hours after ovulation and was accelerated during the following several days. The data obtained in this study differed in some aspects from those in a study of rhesus monkeys. An invasion of the granulosa layer by blood capillaries was seen in the corpus luteum 15 hours after ovulation and the capillaries had already rcached a cavity 37-40 hours after ovulation. Mitotic figures were observed in granulosa and theca interna cells until 43-47 hours after ovulation. It was not impossible to distinguish granulosa cells from theca interna cells in any stage, although it was difficult to do so in some stage.View full abstractDownload PDF (1719K) -
Hiroyasu EJIMA, Tsutomu KUROKAWA, Kenji ABE, Tsutomu KARASAWA, Toshio ...1978 Volume 40 Issue 3 Pages 309-313
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSSeventeen blood samples of chimpanzee were examined for the presence of homologous antibody. All of them contained anti-B but not anti-A antibody. They also possessed A-like antigen on the red cell membrane. After complete absorption of the antibody by human B and AB red cells, the existence of homologous antibody was demonstrated in chimpanzee serum by the bromelin method. This antibody was detected from eleven of the 17 samples tested and tentatively designated anti-Ch antibody. With this antibody chimpanzees could be divided into two types, Ch (+) and Ch (-). Of the 17 chimpanzee samples, two were of Ch (+) type and the other fifteen of Ch (-) type.View full abstractDownload PDF (513K) -
Jesus ARIAS-IBARRONDO, Takeshi MIKAMI, Hiroshi YAMAMOTO, Yuichi FURUTA ...1978 Volume 40 Issue 3 Pages 315-323
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSA virus isolated from the brains of young Japanese sparrow-hawks suffering from an acute infection with a slight nervous symptom was shown to have the morphological, physical and chemical characteristics of a paramyxovirus. The virus was serologically related to Newcastle disease virus and Bangor virus but differed from them in some biological aspects mainly in hemagglutination (HA) activity. The HA reaction of the virus was temperature-dependent. The constant HA reaction was observed when tested at 4°C but not at room temperature or 37°C.View full abstractDownload PDF (1016K) -
Yoshitaka SUZUKI, Shuzo SUU1978 Volume 40 Issue 3 Pages 325-331,334
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe spheroids in the brain and spinal cord were examined lightmicroscopically in 55 dogs (1 month to 18 years of age). Their shape varied from round to oval and was sometimes ellipsoidal or irregularly elongated, and the size ranged from 5 to 200μm in diameter. The spheroids were acidophilic and argyrophilic, histochemically positive for protein reaction but negative for nucleic acid reaction. As a new finding, PAS positive concentric round bodies were found within the spheroids in aged dogs. In the brain, the most constantly affected area was the dorsal column nuclei, especially in the gracile nucleus in the medulla oblongata. In the spinal cord, caudal segments from the lumbar to the cauda equina were noticed as the predilection sites, and the spheroids were more frequently found in the substantia intermedia hnd ventral column. The occurrence of the spheroids tended to increase with age and was marked in chronic wasting disease such as tumor cachexia and severe filariasis.View full abstractDownload PDF (1557K) -
Mitsuo SONODA, Kiyoshi TAKAHASHI, Takashi TAMURA, Masateru KOIWA1978 Volume 40 Issue 3 Pages 335-339,341
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSHaemobartonella canis was observed in the peripheral blood of both intact and splenectomized mongrel dogs inoculated intravenously with Babesia gibsoni derived from a dog affected naturally in Nagasaki City. It was detected on the erythrocytes of 13 experimental dogs, except 7 dogs inoculated with the Babesia strain only for passage and not examined hematologically. The incubation period of H. canis infection was between 3 and 8 days, with 4.7 days on the average, after inoculation. H. canis appeared on erythrocytes on the same day with or a few days earlier than B. gibsoni. Its organisms attached to the surface of the erythrocyte singly, in a raw, or in a cluster, and reddishpurple in color on Giemsa-stained films (Figs. 3-5). In scanning electron microscopy, erythrocytes parasitized with many H. canis were deformed markedly, showing invaginations of the surface membranes. Coccoid, bacillary or doughnut-shaped organisms about 0.2-0.6 μm in diameter were located in grooves (Figs. 6 and 7). In transmission electron microscopy, the organisms were bound with a single trilaminar unit-membrane and packed with much dense granular substance (Fig. 8). This is the first report on the existence of H. canis in Japan.View full abstractDownload PDF (783K) -
Volatile Fatty Acids and pH in the Gastrointestinal Contents of Normal and Shigella-Infected MonkeysMasao TAKASAKA1978 Volume 40 Issue 3 Pages 343-348
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSGas chromatographic analysis of volatile fatty acids (VFA) and determination of pH were performed on the gastrointestinal contents of normal and Shigella-infected monkeys (Macaca fascicularis). The results obtained are as follows. 1) In both normal and Shigella-infected monkeys, the total VFA concentration of the large intestine, especially of the cecum, was evidently higher than that of the stomach and of the lower small intestine. However, the total VFA concentration in the every part of the gastrointestinal tract of Shigella-infected monkeys decreased by about 50% as compared with that of the normal ones except for the stomach. 2) The most predominant VFA was acetic acid, followed by propionic and n-butyric acids in both normal and Shigella-infected monkeys. 3) With regard to pH, Shigella-infected monkeys showed higher values than the normal ones in all parts of the gastrointestinal tract except for the stomach. The largest difference was observed in the cecal contents. 4) There was a statistically significant reciprocal relationship between total VFA concentrations and pH in the contents of the large intestine of normal monkeys. These resiults of VFA and pH are discussed as possible predisposing factors for the establishment of Shigella infection.View full abstractDownload PDF (662K) -
Shigenori IKEMOTO, Yukio SAKURAI, Yoshihiko WATANABE, Hiroyasu EJIMA1978 Volume 40 Issue 3 Pages 349-351
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSDownload PDF (332K) -
Tadao SERIKAWA, Takehiko MURAGUCHI, Noboru NAKAO, Yasuhiro IRIE1978 Volume 40 Issue 3 Pages 353-355
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSDownload PDF (392K) -
Shige KIMURA, Akira SHIMIZU1978 Volume 40 Issue 3 Pages 357-359
Published: June 25, 1978
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSDownload PDF (376K)
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