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[in Japanese]

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A strange disease of the muzzle in cattle is present in the Anrami and Ryukyu Islands.It does not seem to have been described in any published papers. The author started studieson this disease, collecting mater"ials from diseased cattle. The following results were obtained.I . In this disease, the muzzle of cattle changed to a white color, accompanied by erosionand itching. Eventually leul<oderrna occurred. These changes were induced only to themuzzle. It appeared that cattle were attacked by this disease at 4 months of age and passedinto chronic state, and the resultant leukoderma concinued for a long time. lhe diseaseoccurs in a greate number of cattle, but in no other anixnals.2. Flistopathological changes were seen mainly in the papillary and reticular layer"s ofthe skin. Infiltratiorn of eosinoplails and round cells and production of granulation tissuewere observed. These lesions proceeded to the epithelium, and eventually tlne muzzlesuffered frotm Ieul<oderrna as a result of disappearance of melanin. Round cells infiltratedinto not only the corium bttt also the tela subcutanea. Some adult worms of nematodesand many rnicrofilariae were observed in lesions of lnistological sections.The cause of this disease was found to be this worm. ?F his lesion was of parasitiedertnatitis and a diagnosis of leukoderma was made to its presence. Leukoderrma dtue toparasitic dermatitis is of great interest.3. Many adult worms and rnicrofilariae were obtained from Iesions by cuttirng the skininto small pieces in a Petri dish filled with normal saline. The female was 7.6 to 9.1 1111111in length, the rnale from 3.2 to 3.9 mm, and the nnicrofilaria 70 to 90 p.Morphological characters are shown in figures. Comparison of principal measurementsin each species is shown irn tables. It is obvious that 1., his worm resembles, 5. dedoesi, S.assamensis, , S. stilesi and .S. kaeli rnentioned in references, although there are some differ"encesbetweerr this worm and the others. Especially characteristic of this worm is its parasitismr

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Studues were conducted to elucidate the part of the patlao-physiology of body fluids inthe dehydration of ruminants, using 5 healthy goats which had been brought into a stateof water depletion Lay cessation of water intake and feeding on a dry ration only.Total body water, extracellular water, and plasma volume were measured simultaneously, ustng NAAP, NaSCN, and T -1824, respectively. The maximum reduction of the body fluidcompartments at the last stage was 43.5% in total body water, 33.8% in extracellular water, 52.9% in intracellular water, 31.1% in interstitial water, 47.6% in plasma water, and 37.6%in circulating blood volume.In view of the fact that the body weight was reduced significantly in the early stage, whereas there were no appreciable changes in the body fluids, it was presumed that thedecrease of body weight at this time was due mainly to the reduction of the water contentin the rumen. The deficiencies of total body water as expressed in per cent of the initial body vveightwere 2.7% in the early stage, 16.9% in the middle stage, 23.8% in the late stage, and 26.9%in the last stage.The rate of decrease of interstitial water (8.8%) was higher than that of intracellularwater (0.4%) in the early stage. This condition, however, was reversed in and after themiddle stage.

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Imrnunochemical studies 1?ave been performed in the field of veterinary science, especiallyto clarify immunity against parasitosis. Many problems, ltowever, remain to be solved.They include not only a question on the enforcement of immunization procedure but alsoOne on the possibility of immunization against parasitogenic diseases.The author has already inxxestigated anti-antigen fractions against the antigen whichwas extracted from the homogenized tissue of canine filariae and which consisted mostly ofprotein and polysaccharide. These fractions were contained in the components of fB- and7-globulin in the serum.Besides, preliminary expen"iments were carried out to investigate the amino-acid organi-zation of the antibody. As a result, a considerably peculiar variation was observed in freeamino acids in the serum in the case of parasitism of canine filariae and a few otherparasitoses.For qualitative and quantitative determination of free amine acids in the serum, paperand column chromatography have been utilized. In the analysis of amino acids, high voltagepaper electrophoresis differs from ordinary electrophoresis. The former is less partial thanthe latter as to the length of tirne required for clinical symptoms to appear, the accuratepattern and the speed of analysis.Therefore, as one of the fundamental studies by high voltage paper electrophoresis, anexperiment was made at first on the relative order of migration of 19 kinds of crystal arninoacids which had been dissolved in acid liquid. In the experiment, filter paper was hungfrom the anode to the cathode in the receptacle of development liquid consisting of formicacid and acetic acid (pH 1.5). Frorn this experiment, it, ?vas confirmed that there weremigrating basic arnino acids on the cathode side, separated acid amino acids with two neutralamino acid components (Phe and Cys) toward the anode sidcc, and the main neutral aminoacid components, which were organized by four fractions, between two groups of acid andbasic amino acids as stated above.Strictly spe

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