Pathological investigation was carried out on 10 cases of lymphosarcoma in swine. Cytologically, neoplastic cells in these cases presented essentially the same morphological characteristics as lymphocytes. Neoplastic lesions were recognizable predominantly in lymph nodes, liver, spleen, kidney and urinary bladder, and additionally in many other organs macroscopically or histologically. They were remarkable in tonsil, genital organs, bone marrow, nervous tissue, pancreas, adrenal, hypophysis, tongue, submaxillary gland and gallbladder. Their histogenesis was suggested to consist in the accumulation of neoplastic cells throughout perivascular areas, judging from the character of these cells.

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Salmonella typhimurium, Copenhagen variety was isolated from 13 domestic pigeons (Columba livia domestica) of 4 pigeonries in which clinical salmonellosis occurred during the period of 1970 to 1975. Of 58 composite fecal samples collected from feral pigeons (Columba livia domestica) in 13 districts of Honshu and Hokkaido in 1975 and 1976, 12 (20.7%) from 3 districts gave the same antigenic form. The antigenic form was also isolated from the tissues of 2 (1.8%) feral pigeons (Columba livia domestica) caught at one site among a total of 110 feral pigeons (80 Columba livia domestica and 30 Streptopelia orientalis orientalis) caught in 3 districts of Hokkaido in 1976. No Salmonella other than S. typhimurium, Copenhagen variety was obtained from pigeons. These isolates fermented maltose, and belonged to biovar 10 [2]. All the isolates from feral pigeons and most of those from domestic pigeons showed in-vitro drug sensitivity. S. typhimurium, Copenhagen variety having the same characteristics was isolated from animals, chickens and wild birds, though the antigenic form originating from hosts other than pigeons was drug resistant with variable frequencies, and different biovars were found in some hosts. This appears to indicate that pigeons have a role in the epizootiology of salmonellosis in animals and birds in Japan.

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Canine oral papilloma cells were cultivated in vitro by the trypsinization procedure. The success of primary cell culture depended on the choice of a proper stage of papilloma growth. Dome-shaped papilloma was suitable for cultivation. Cultivated cells were flat, and round or polygonal in shape. They were piled up or overlapped, and keratinized cytologically. From the normal mucosa, fibroblasts and epithelial cells were cultivated only by explant culture, while the epithelial cells were neither keratinized nor piled up.

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In both bled and non-bled dogs under ketamine hydrochloride anesthesia, sinus tachycardia was sustained, but no particular irregularities were found in the cardiac rhythm. After ketamine anesthesia somewhat apparent changes occurred to ECG waves and ST deviation and the P-Q and Q-T interval (lead II) were relatively prolonged in the bled dogs, as compared with the non-bled dogs. The changes in EEG waves during ketamine anesthesia were of essentially the same pattern in the bled dogs as in the non-bled dogs, although the amplitude was lower in the former than in the latter.

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A total of 1, 186 dogs from Gifu and Shiga area were surveyed for Brucella canis (B. canis) during a year from April, 1976 to March, 1977. The dogs with serum agglutinin titer of 1:320 or higher against B. canis were sacrificed and their various organs and fluids were cultured for the organisms. Through the year, detection rate confirmed by isolation of B. canis was 2.8% (33 dogs) and no statistically significant difference was found between the two groups (2.5% in Gifu and 3.5% in Shiga), nor between males and females (16 and 17, respectively). Monthly detection rates of infected dogs were fluctuated from 0.8 to 5.5%, but no clear seasonal influence was found. Among the surveyed dogs there were 188 pure bred dogs including 123 dogs weighing more than 20 kg, but non of them was positive for B. canis. All positive dogs were mongrel. B. canis was isolated from various organs, most frequently from the prostate and bladder urine for males and from the lumen of uterus for females in addition to the spleen and lymph nodes of both sexes. B. canis was isolated from bladder urine in 14 out of 16 males, but only 5 out of 17 females by direct cultural method. The number of viable B. canis in bladder urine was more abundant in males than in females. The importance of urine as one route of dissemination of the organism was discussed.

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Morphological investigations were carried out on the eye balls of domestic animals, 16 eye balls in horses, 28 in cattle, 26 in goats, 30 in swine, 18 in dogs, 20 in cats and 28 in rabbits. These materials were used for measurements of the portions of the organ. Additionally, 8 eye balls in horses, 22 in cattle, 14 in goats, 18 in swine, 20 in dogs, 26 in cats and 16 in rabbits were examined by scanning electron microscope. The results of measurtment of each portion of the eye appeared species specificity for the animals, as shown in Table 1. Characteristic morphological features of the organ in each species of the animals were obtained by the scanning electron microscopy. The morphology of the iris and ciliary processes were different among the species of the animals. Pupillary granules were present in the swine and dog, in addition to the horse, cattle and goat, and revealed characteristic shapes for each species of the animals. Zonular ligaments were composed of fibrous bundles of various thickness, and the extent of those fibers was different in each species. The shape of the optic disc revealed also specific shapes in the each species of the animals. Hyaloid process was projected in the center of the optic disc of the cattle and goat. The structure of the lens was generally identical among the animals examined. Numerous small holes were observed on the surfaces of lenticular fibers.

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