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Volume 33 (1971)
- Issue 6 Pages 277-
- Issue 5 Pages 209-
- Issue 4 Pages 161-
- Issue 3 Pages 111-
- Issue 2 Pages 53-
- Issue 1 Pages 1-
Volume 33, Issue 5
Displaying 1-6 of 6 articles from this issue
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Katsuya HIRAI, Seigo SHIMAKURA1971 Volume 33 Issue 5 Pages 209-216_1
Published: October 25, 1971
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSThe nephrosis-inducing infectious bronchitis virus (IBV) was isolated on primary chicken embryo kidney (CEK) cell culture from the kidney lesion of chickens. The cytopathic effect (CPE) was produced only by blind passages of the virus in CEK cell cultures after the adaptation of the virus to embryonating eggs. The maximum infective titer in culture medium appeared in 48 hours after inoculation. The virus was sensitive to ether, chloroform, and sodium deoxycholate, stable at pH 3.0, and was inactivated after heating at 56°C for 45 minutes. No replication was inhibited by 5-bromodeoxyuridine. The virus passed through filters 100mμ or more in average pore diameter, but not through filters 50mμ in such diameter. Its viral particles negatively stained exhibited pleomorphism, were 110mμ in average diameter, and had club-shaped projections on their surfaces. No nucleocapside structure was seen in the virion. Thus, the virus exhibited such properties as typical of coronavirus. It was neutral-ized by the known IBV strains. It is therefore concluded that the virus inducing nephrosis in chickens is the nephrosis-inducing type of IBV in Japan.View full abstractDownload PDF (1552K) -
Haku SUGAWARA, Jinichi SASAKI1971 Volume 33 Issue 5 Pages 217-226
Published: October 25, 1971
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSDownload PDF (942K) -
Kiyoshi TSUNODA, Yaichi MURAKI1971 Volume 33 Issue 5 Pages 227-235_2
Published: October 25, 1971
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSA species of coccidium belonging to the genus Eimeria was isolated from Japanese quails. A pure strain of this species was obtained by the single oocyst infection method. Then the whole life-cycle of this species was clarified by experimental infection. This species was affirmed to be a new one and named Eimeria uzura. E. uzura was mainly located in the duodenum and the upper part of the small intestine. This species resembled E. coturnicis and E. bateri in the morphology of the oocyst except the presence of the micropyle. The sporulation time of E. uzura was 22-24 hrs. at 25°C. The prepatent period was 88-96 hrs. after infection and the patent period was around 13 days. Detailed examination, however, revealed some important differences among these three species. Experimental infection with E. uzura failed in 11 different species of birds which are taxonomically colse to the Japanese quail. Twenty species of Eimeria which attacked the 11 species of birds were never infective to the Japanese quail.View full abstractDownload PDF (1837K) -
Kazuhiko YAMADA1971 Volume 33 Issue 5 Pages 237-249
Published: October 25, 1971
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSIn man, the presence of aspartate-aminotransferase (GOT) and alanine-aminotrans-ferase (GPT), which are commonly known as the transaminases, is valued as a clue for the rapid diagnosis of myocarditis and hepatitis. The reversible transamination reactions by these transaminases, generally known in the replacement of =C=O with NH3, are essential for the utilization of nitrogen in living materials. So far as the author is aware, no detailed investigations have been conducted on the activities of GOT and GPT which are contained in the fowl blood plasma. For the purpose of studying this problem, the author tried to classify the abovementioned transaminases into categories bearing such terms as forward-GOT(F-GOT), reverse-GOT(R-GOT), forward-GPT (F-GPT), and reverse-GPT(R-GPT). Their activities were estimated by the atuhor's method, which was the same in principle as that reported by REITMAN and FRANKEL. The systems which are catalyzed by these transaminases are as follows.View full abstractDownload PDF (1065K) -
Hiroshi OIKAWA, Harumoto KAWAGUCHI, Kiyoshi TSUNODA1971 Volume 33 Issue 5 Pages 251-259
Published: October 25, 1971
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESSPathophysiological changes were examined in chickens experimentally infected with Eimeria tenella and E. acervulina in the course of infection and recovery. Following the discharge of bloody feces on the 4th day of E. tenella infection, a marked decreases was found in such blood components as the erythrocyte count, hemoglobin concentration, and total plasma protein concentration until the 10th day. Anorexia, retarded growth, enlarged spleen and liver, and pathological changes of the caeca were also observed in the infected birds. It took about ten days after the onset of discharge of bloody feces for these birds to return to normal growth. Prolongation of retarded growth and decrease in weight of the heart were striking findings in E. acervulina infection. Most of the clinical and pathophysiological changes were observed on the 3rd and 10th day after infection. The decrease in the components of blood was lower in severity but lasted a little longer in E. acervulina infection than in E. tenella infection.View full abstractDownload PDF (706K) -
Michio MORITA, Shigekatsu YOSHIZAWA, YUji INABA1971 Volume 33 Issue 5 Pages 261-270_4
Published: October 25, 1971
Released on J-STAGE: February 13, 2008
JOURNAL FREE ACCESS1) Two spontaneous cases of a disease probably caused by Chlamydia were recognized among cynomolgus monkeys (Macaca irus) imported from the Philippines. They were diagnosed as such from light- and electron-microscopic findings. The diagnosis was also supported by the results of serological and virological examinations. 2) The two cases were pathologically characterized by pleuropneumonia and the ulceration of the tongue and lips. In the electron-microscopic examination, numerous reticulate bodies and elementary bodies were recognized in the cytoplasm of epithelial cells in the lesion of tongue ulcer which contained inclusion bodies observed by light microscopy. Furthermore, Chlamydia-like particles were seen in the lungs.View full abstractDownload PDF (3233K)
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