1. A serial investigation was made on the fate of reinoculated avian tubercle bacilli and on the development of lesions in reinoculated rabbits. 2. The effect of sensitization on the multiplication of bacilli differed from organ to organ. It was the most conspicuous in the liver, spleen, and bone marrow, and was obscure in the lung and kidney. 3. Modification of pathological features in various organs of the reinoculated rabbits was discussed.

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1) In 29 cases of heartworm disease, the average level of serum ionic calcium was 3.9±0.23mg/dl. This value was lower than 4.2±0.24mg/dl, the average level of serurn ionic calcium in the healthy dogs examined. 2) In 6 cases of hepatitis, the average level of serum ionic calcium was 3.7±0.18mg/dl. This value waS significantly lower than 4.2±0.24mg/dl, the average level of the calcium in the healthy dogs. 3) In rabbits which had been injected with carbon tetrachloride in a large net quantity, or 1cc per kg of body weight, the serum ionic calcium exhibited a sharp decline over a period of 24 hours after the injection. Then it showed a gradual recovery in some of these rabbits. A number of rabbits in which the serum ionic calcium had revealed no sign of recovery was led to death without exception. 4) In rabbits which had been injected with a medium quantity, or 0.5cc per kg of body weight, of carbon tetrachloride three times, the serum ionic calcium declined after the first injection and soon it began to recover. After the second injection it repeated the same change. After the third injection, however, it remained at a remarkably low level and was never ameliorated until subsequent death. 5) In rabbits which had been injected with a small quantity, or 0.1cc per kg of body weight, of carbon tetrachloride three times, the serum ionic Calcium declined after the first injection and soon it began to recover. With repeated injections, such alternation of decline and recovery became more and more frequent. The serum ionic calcium reached a comparatively low level 40 days after the first injection. 6) Total serum protein and total leukocyte count varied simultaneously in the above-mentioned experiments. They showed a marked decline in rabbits under acute conditions of disease terminating in death. 7) In histopathological examination, those cases which had liver damage were found to be in a condition characterized by hypo-ionic calcemia.

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Changes in serum protein were investigated in dogs infected with Ancylostoma caninum by using paper electrophoresis. Among changes in the serum protein components which occurred as a result of natural and experimental infection, a tendency was observed that albumin decreased and the globulin fractions increased relatively. In severe ancylostomiasis, alteration in the serum protein fractions was strongly in evidence and a marked rise in alpha-2 globulin was especially recognized. The relations of the serum protein changes to age and severity of anemia were studied. It was observed that the serum protein changes mentioned above were more significant in puppies and young dogs than in adult dogs both in natural and experimental infection. Moreover, the albumin fraction showed a remarkable decrease with progress in anemia. On the other hand, a remarkable rise was observed in the alpha-2 glycoprotein fraction in severe cases, but no notable changes were found in the lipoprotein fractions. Gros test revealed an increase of the positive cases in severe ancylostomiasis, meanwhile no remarkable changes were observed in serum total cholesterol values. Dogs subjected to experimental anemia due to bloodletting exhibited a decrease in the albumin fraction and a marked increase in the alpha-2 globulin in the serum. These changes in the protein fractions occurred immediately after bloodletting.

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