Background & Aims: To investigate whether the optimal treatment protocols of cryoablation differ among various cell lines utilizing bioluminescence imaging (BLI).
Methods: Eight luciferase-expressing cancer cell lines originated from various organs were used in this study, including lung, renal, liver, breast, colon, prostate, and skin. These cell lines were uniformly seeded in 96-well plates. The cell lines were then divided into four groups: Group 0 (G0), as a control group, and three treatment groups (G1-G3). G1-G3 were subjected to cryoablation at temperatures of -45℃, -60℃, and -80℃, respectively, with varying treatment cycles (single, double, or triple cycle, respectively). Each freeze‒thaw cycle consisted of ten minutes of freezing followed by five minutes of thawing. Finally, the average radiance of the cells was assessed using BLI.
Results: Cryoablation at “-60℃ and -80℃” showed a significant decrease in average radiance, even after a single cycle of treatment in all cell lines. The average radiance of cells treated with -45℃ was substantially lower than that of untreated cell lines except melanoma.
Conclusion: We found that -45℃ successfully eradicated all cancer cells across multiple cycles except melanoma. The most effective cryoablation protocol for all cell lines was observed at temperatures of -60℃ or lower.
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