The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Volume 29, Issue 1
Displaying 1-6 of 6 articles from this issue
  • II. DEDIKARYOTIZATION AND ITS GENETICAL IMPLICATION
    TSUNEO TAKEMARU
    1954 Volume 29 Issue 1 Pages 1-7
    Published: 1954
    Released on J-STAGE: November 30, 2007
    JOURNAL FREE ACCESS
    Cytological observations on the dedikaryotization process in C. velutipes and mating type analysis of the dedikaryotized oidia were done with the results confirming the Brodie's. report (1936).
    A new convenient way for comparative studies in cytoplasmic and nuclear influences by means of the dedikaryotized oidia is suggested (Fig. 16).
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  • Y. KURODA
    1954 Volume 29 Issue 1 Pages 8-12
    Published: 1954
    Released on J-STAGE: November 30, 2007
    JOURNAL FREE ACCESS
    Amylases of the digestive fluid and of the mid-gut tissue extract in the fifth-instar larvae of N-115 and of ae-2 strain of the silk worm, Bombyx mori, were extracted and concentrated by precipitation with acetone and ammonium sulfate. Their activities were compared as follows:
    1) The amylase activity of the digestive fluid of N-115 larvae was fifty times higher than that of ae-2 larvae, while its activity of the mid-gut tissue extract of N-115 larvae was shown to be only five times higher than that of ae-2 larvae.
    2) The optimal pH for amylase activity of the digestive fluid of N-115 larvae was shown to be 9.2, that for the mid-gut tissue of N-115 larvae being 9.8.
    3) The paper chromatography of amylases in the digestive fluid and the mid-gut tissue of N-115 larvae was performed. Amylases were developed by 5-10% acetone, but the treatments with sodium chloride, ammonium sulfate and phosphate buffer (pH 9.6) failed in the attempt. The Rf values of amylase in the digestive fluid were 0 and 0.66, while those in the mid-gut tissues being 0, 0.44 and 0.61.
    4) The histological observations of the mid-gut tissues in the fifth-instar larvae of both N-115 and ae-2 strains were carried out. Many secretory granules were observed between the striated borders and the peritrophic membranes of the mid-gut tissues not only in N-115 but also in ae-2 strain. The granules in the fifth-instar larvae seem to be increased in numbers and volumes after three days.
    According to the above results, we can assume that the amylase seems to be secreted from the mid-gut tissue of both N-115 and ae-2 strains, but its activity in the digestive fluid is varied when amylase passes through the peritrophic membrane. The difference of amylase activities in the digestive fluid between N-115 and ae-2 strains is supposed to depend on the secretory amount of amylase and on the permeability of the striated border.
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  • S. MATSUMURA, T. FUJII
    1954 Volume 29 Issue 1 Pages 13-17
    Published: 1954
    Released on J-STAGE: November 30, 2007
    JOURNAL FREE ACCESS
    Dormant seeds of Triticum monococcum were exposed to ultra short electric waves. Impulse waves were applied (wave length 10m) to remove the heat effect, at 5, 100V, 10mA current and about 8cm distance. Few chromosomal irregularities were observed in the meiosis of the treated plants (S1-generation). Among the progeny (S2) obtained by selfing of the S1-plants, however, there were found some dwarf plants, which had about half the height of normal plants and were highly sterile. In the next generation (S3) of the sister plants of the dwarfs, again some sterile dwarfs appeared, but their height and fertility were somewhat improved. Crossing of these dwarfs with T. monococcum and aegilopoides was unsuccessful. In the S4-generation recovery of fertility and plant height wass further considerably increased and at last, in the S5-generation no dwarf could be found. The mechanism of these phenomena awaits further investigation.
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  • II. INDIVIDUAL VARIATION IN TETRAPLOID RICE
    H. OKA
    1954 Volume 29 Issue 1 Pages 18-25
    Published: 1954
    Released on J-STAGE: November 30, 2007
    JOURNAL FREE ACCESS
    1. Variations among plants of the same variety in fertility, plant height and heading date were observed on different generations of five tetraploid varieties. In general, tetraploid plants showed a wider range of variation than diploid plants.
    2. Having compared the variation among lines with that within lines (a line means plants raised from the seed of a plant of the preceding generation) by the analysis of variance, the variation among lines was in a greater part of cases found to be significant. In addition to this, significant correlations were found in some case between progeny and parent. Individual variation within a tetraploid variety seems, therefore, to be at least partly heritable.
    3. However, on account of the variation arising continually in every generation, selection seemed ineffective, and the average of measurements for a number of plants was recognized to be a character peculiar to the given variety. Fertility seemed not to be improved with the lapse of generations.
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  • G. GOTO, A. ONUKI
    1954 Volume 29 Issue 1 Pages 26-35
    Published: 1954
    Released on J-STAGE: November 30, 2007
    JOURNAL FREE ACCESS
    From calculations on the standpoint of genic equilibrium, authors obtained the following results; diathesis of susceptibility to tuberculosis is inherited by two recessive independent genes a and b, a is of severe illness, of which the homozygotes fall ill entirely, b is of mild illness, of which bb individuals fall ill with some genotypic morbidity α. The frequecy of aa is 0.044 and that of bb is 0.21.
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  • S. TAKASHIMA
    1954 Volume 29 Issue 1 Pages 36-39
    Published: 1954
    Released on J-STAGE: November 30, 2007
    JOURNAL FREE ACCESS
    1) When we compare the interspecific degree of the pollen tube growth, we find that in three species the speed of the pollen tube growth in the stigma is most rapid and it becomes slower in the style and in ovary it is slowest. The reason of above mentioned phenomenon is probably this that in Cucurbitaceae a certain substance as that inhibitory substance which is held to be the cause of self-incompatibility makes its influences even over the pollination between the same species.
    2) When we investigate the inhibitory substance which is held to be on one cause of relative difficulty in crossing, we can find that the inhibitory substance engendered in the ovary since two days before the flowering begins to move upwards and on the day before the flowering centers of certain kind of hormone in the stigma (this may be thought as a generation of Wund-Hormon by the excitation of pollination) or the diminution of the inhibitory power, the inhibitory substance in the stigma does not make notable influence over the pollination between the same species.
    3) The inhibitory substance continues to generate even on the flowering day.
    4) To investigate the hormone problem mentioned above in (2), we made the following experiment. When we took off the stigma from the pistil and on the amputated pistil we applied the vegetable hormone and pollinated it, we could recognize nearly the same growth. So, as a conclusion, we may say that on the flowering day, a certain hormone generates in the stigma and that hormone promotes the growth of the pollen tube.
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