Amylases of the digestive fluid and of the mid-gut tissue extract in the fifth-instar larvae of
N-115 and of
ae-2 strain of the silk worm,
Bombyx mori, were extracted and concentrated by precipitation with acetone and ammonium sulfate. Their activities were compared as follows:
1) The amylase activity of the digestive fluid of
N-115 larvae was fifty times higher than that of
ae-2 larvae, while its activity of the mid-gut tissue extract of
N-115 larvae was shown to be only five times higher than that of
ae-2 larvae.
2) The optimal pH for amylase activity of the digestive fluid of
N-115 larvae was shown to be 9.2, that for the mid-gut tissue of
N-115 larvae being 9.8.
3) The paper chromatography of amylases in the digestive fluid and the mid-gut tissue of
N-115 larvae was performed. Amylases were developed by 5-10% acetone, but the treatments with sodium chloride, ammonium sulfate and phosphate buffer (pH 9.6) failed in the attempt. The Rf values of amylase in the digestive fluid were 0 and 0.66, while those in the mid-gut tissues being 0, 0.44 and 0.61.
4) The histological observations of the mid-gut tissues in the fifth-instar larvae of both
N-115 and
ae-2 strains were carried out. Many secretory granules were observed between the striated borders and the peritrophic membranes of the mid-gut tissues not only in
N-115 but also in
ae-2 strain. The granules in the fifth-instar larvae seem to be increased in numbers and volumes after three days.
According to the above results, we can assume that the amylase seems to be secreted from the mid-gut tissue of both
N-115 and
ae-2 strains, but its activity in the digestive fluid is varied when amylase passes through the peritrophic membrane. The difference of amylase activities in the digestive fluid between
N-115 and
ae-2 strains is supposed to depend on the secretory amount of amylase and on the permeability of the striated border.
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