The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Volume 35, Issue 5-6
Displaying 1-4 of 4 articles from this issue
  • Minoru SHIMOTSUMA, Yoshito OGAWA
    1960 Volume 35 Issue 5-6 Pages 143-152
    Published: 1960
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    The bitter substance in the fruits of C. colocynthis and C. vulgaris was analysed by chromatographic technique. Only one compound was detected in the ripe fruit of C. No. 1 (2x & 4x). The junior author identified it as “Citbittol A”. The amount of bitter substance in the ripe fruit of tetraploids was nine times as high as that of diploids.
    In unripe fruits of C. No. 1 (2x), however, seven new bitter compounds were detected. These newly found bitter substances were called “Citbittol B, C, D, E, F, G and H”. The amount of bitter substance in unripe fruits was higher than that of the ripe fruits. The bitter substance in the unripe fruit decompose gradually as the fruits ripen. Four compounds were detected in the ripe fruits of V. No. 2 and 4. Although C. No. 4 belongs to C. colocynthis, bitter substances was not found in the ripe fruits in this exceptional case.
    Ripe fruits of di-, tri- and tetraploid interspecific hybrids obtained from all possible cross combinations among diploid and tetraploid strains of C. No. 1 and V. No. 1 contain a higher amount of bitter substance in the ripe fruit than C. No. 1.
    The segregation ratio 3 (bitter): 1 (non-bitter) for F2 population and 1 (bitter): 1 (non-bitter) for the testcross was indicated by chromatographic analysis of bitter substance in the ripe friuts of each individual. From this evidence, bitterness in the genus Citrullus was concluded to have monohybrid inheritance with dominance of the bitter character.
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  • Takumi TSUCHIYA, Jiro HAYASHI, Ryûhei TAKAHASHI
    1960 Volume 35 Issue 5-6 Pages 153-160
    Published: 1960
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Thirty five populations of F2 and 5 of F3 families were tested for disomic and/or trisomic inheritance. In these experiments 10 gene pairs were tested in 6 primary simple trisomic types with the following results:
    1. Blbl gene pair in 5 trisomic types of Bush, Slender, Pale, Robust, Purple, Brbr pair in Pale, and Rr in Purple and Semi-erect were newly tested for segregation ratios. Only Blbl in Robust and Rr in Semi-erect showed the trisomic ratio while in the others the disomic ratio was observed.
    2. Out of 35, 27 F2 populations tested in the present experiment showed to agree very well with the previous results obtained from the same combinations (cf. Tsuchiya 1956, 1959): Namely, Nn (III), and Fcfc (VII) in Bush, Vv (I) in Slender and Uzuz (VI) in Pale showed to be fitted very well with the expected trisomic ratio in both the diploid and the trisomic portions. The other showed disomic segregation.
    3. Segregation of Kk gene pair was tested in 5 F3 families derived from trisomic F2 plants from the Robust×Colsess V cross. The results showed that the egregation of Kk gene pair in both diploid and trisomic portions occurred in the trisomic ratio. Thus, the Robust trisomic for chromosome 4 (Burnham's e) showed to carry the genetic linkage group IV on its extra chromosome.
    4. From all these findings the relationships between the seven primary simple trisomic, types and the seven known genetic linkage groups of barley have been established (Tables 5 and 6).
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  • Haruo KUROKAWA
    1960 Volume 35 Issue 5-6 Pages 161-166
    Published: 1960
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    The three races, A. B, and C composing Drosophila auraria have been discussed with regard to sexual isolation and speciation problem.
    Intraracial sexual isolation between different geographic strains of race C, regardless of the strains used, is not significant, while interracial sexual isolation, in every case between A and B, A and C, and B and C, is significantly demonstrated.
    Genetically diverged distances in relation to the coeffcients of joint isolation and some characteristics of the three races are respectively illustrated in the Fig. 1.
    It can hardly be concluded from the morphological, physiological and distributional standpoints, that, of the members belonging to species auraria, race B would be an original form of them.
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  • III. Meiotic abnormalities caused by water extracts from Arisaema japonicum
    Rinjiro OHNO, Sigeyuki TANIHUZI
    1960 Volume 35 Issue 5-6 Pages 167-176
    Published: 1960
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    This is a report on the cytological effects of water extracts of Arisaema japonicum on PMCs of Trillium kamtschaticum Pall. (2n=10, 511).
    The water extracts were prepared by immersing 10, 20, and 40 grams of ground bulbs of Arisaema into 100cc of the distilled water for 12 hours at 0°C. The flower buds of Trillium, after the removal of sepals and petals, were immersed into the extract for 3 or 6 hours. After the treatment the PMCs were fixed for observation every other day.
    The major results obtained are summarized as follows:
    1) Immediately after the treatment, the following aberrations were observed: the clumping and clotting of chromosomes at prometaphase or at MI; the over-contraction, slight stickiness of chromosomes at MI and at AI.
    2) A few days after the treatment, the chromosomal anomalies observed are: the chromosome lagging and their disturbance in migration to poles. In some TI cells, the lagging of one univalent was observed. The lagging of two homologous univalents was of rare occurrence. The precocious separation of chromatids of the lagging univalent(s), though it was considerable frequent in the streptomycin-treatment, was not observed at all.
    There occurred abnormal cells at TI about 23 per cent which showed non-convergence of chromosomes at one or both polar regions. It seems probable that the abnormality, might result from the upset of the polar function caused by the extracts. The close juxtaposition of the two groups of chromosomes at TI and at interphase, unequal separation of chromosomes at AI and at TI, and the abnormality in formation of the cell wall were also recognized.
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