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Volume 40 (1965)
- Issue 5-6 Pages 343-
- Issue 4 Pages 261-
- Issue 3 Pages 159-
- Issue 2 Pages 87-
- Issue 1 Pages 1-
Volume 40, Issue 1
Displaying 1-9 of 9 articles from this issue
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ZEN-ICHI OGITA, TSUTOMU KASAI1965 Volume 40 Issue 1 Pages 1-14
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSSoluble esterases which hydrolyse β-naphthylacetate have been prepared from the 10 laboratory strains of the house fly and separated into 24 electrophoretic bands by Ogita's thin layer electrophoretic technique. First, these esterase bands were distinguished by electrophoretic mobilities. They were classified into enzymatic families on their behavior differences. The differences included changes in the pattern of esterases during ontogeny, and the heterogeneity with regard to substrate specificity and inhibitor susceptibility as well as the localization of esterases in various tissues. Linkage tests have shown that one of the bands might be controlled by a genetic factor on the 4th chromosome, and the others by genetic factors on the 5th chromosome. Genetic analysis of these esterase bands indicates that the loci responsible for the two esterases are located on the 5th chromosome. They were named as Est-A and Est-B loci. A recombination value between the cm and Est-A loci is approximately 20.7±and that between the cm and Est-B loci is 50.6±mapunits.
The esterase bands which are active upon naphthylacetate esters are not correlated with the diazinon-resistance gene.View full abstractDownload PDF (1885K) -
MASAHARU EGUCHI, NARUMI YOSHITAKE, HIDENORI KAI1965 Volume 40 Issue 1 Pages 15-19
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSBy means of thin layer electrophoresis in agar gel, the zymograms of blood esterase of 161 silkworm strains was studied. Four fundamental types of esterase were found, and crossing experiments led to the conclusion that they are controlled by codominant alleles, BesA, BesB, BesC, and BesO. About 70% of the Japanese, Chinese and European races investigated belong to A type and 20% to O type, while B type was found only in Chinese race.View full abstractDownload PDF (639K) -
AKIRA YUASA, MASAMI SONEDA, MASAKO OSUMI, MIEKO SUMIYASU1965 Volume 40 Issue 1 Pages 21-24
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (846K) -
I. CYTOLOGICAL OBSERVATIONSTAIRA KATAYAMA1965 Volume 40 Issue 1 Pages 25-32
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSIn cytological studies at diakinesis and at dia-metaphase of the diploid japonica type rice variety “Fukui-Ginbozu”. chromosomal associations of 3(3)+3(1), 3(3)+1(2)+1 (1), 4(3), 6(2) and others, showing definite deviation from the supposed maximum association, 2(3)+3(2), were observed frequently.
In this connection, a review of the previous works on the subject of so-called secondary association was presented.View full abstractDownload PDF (1432K) -
II. STATISTICAL ANALYSISAKIO KUDO, TAIRA KATAYAMA1965 Volume 40 Issue 1 Pages 33-44
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe data presented in the preceding paper Katayama (1964) are analysed on the basis of three types of randomness of associations: general random association, linear random association and circular random association, with the object of examining whether the data support the hypothesis of the prime number of chromosomes being 5. Although the association does not seem to be random in the sense of any one of the three types of randomness, the results of the analysis do not support the hypothesis of prime number of chromosomes being 5.View full abstractDownload PDF (827K) -
HITOSHI KIHARA1965 Volume 40 Issue 1 Pages 45-54
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (834K) -
SHIGEKATSU TSUJI, TOSIHIDE H. YOSIDA1965 Volume 40 Issue 1 Pages 55-62
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESS1. Reciprocal transplants of dorsal ectodermis between adult hairless mice and their normal litter mates were investigated in two hairless mutants, rhino and furless.
2. Of a total of 56 grafts in which all eight combinations survived the operation (Table 1), 40 cases were completely successful.
3. Successful grafts of non-rhino skin to rhino host behaved autonomously and also rhino grafts on non-rhino hosts behaved autonomously except for a temporary hair growth on the outer border of the graft observed about three weeks after the operation.
4. Furless skins grafted on non-furless hosts were depilated once immediately after operation and regrew after a month, but the second depilation occurred irrespective of the donor's periodicity. The non-furless skin on furless host was not affected by the host, on the contrary the body hair of furless host behind the graft remained unchanged, while the area in front of the graft was depilated in regular fashon.View full abstractDownload PDF (773K) -
YUKIO HUZIWARA1965 Volume 40 Issue 1 Pages 63-71
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe chromosome numbers and the karyotypes of 11 species and 4 varieties belonging to 6 genera of the tribe Astereae of Compositae are reported. These are: Felicia bergeriana O. Hoffin, 2n=18; Erigeron speciosus DC., 2n=18; Solidago minutissima (Makino) Kitamura, 2n=18; Boltonia asteroides L'Her., 2n=27; Rhynchospermum verticillatum Reinw., 2n=18; Aster taiwanensis var. lucens Kitamura 2n=18; A. ageratoides subsp. leiophyllus var. ovalifolius Kitamura, 2n=54; A. sohayakiensis Koidzumi, 2n=18; A. rugulosus var. shibukawaensis Kitamura et Murata, 2n=18; A. dubius subsp. glabratus var. heterotrichus Kitamura, 2n=18; A. asperulus Nees, 2n=54; A. spinosus Benth., 2n=18; A. oregonensis (Nutt.) Cronq., 2n=18; A. bernardinus Hall, 2n=36; A. ontarionis Wieg., 2n=40.View full abstractDownload PDF (694K) -
YASUMOTO KIKUCHI1965 Volume 40 Issue 1 Pages 73-80
Published: 1965
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe present study describes in detail the autoradiographical technique for the study of human chromosome replication. The procedure is as follows.
Leukocytes in the peripheral venous blood taken from human subjects, freshly isolated from the individual using phytohemagglutinin, were cultured in suspension for 72 hours. Tritiated thymidine was added to the cultures at various times to a final concentration of 1μc per ml. After incubation for 10 minutes with the labeled thymidine, the cells were washed and reincubated in a fresh culture medium containing a large excess of unlabeled thymidime. Slides were prepared according to the air-drying technique. The cells were stained with acetic orcein. After staining, all slides were examined under the microscope. In metaphase cells suitable for analysis the chromosomes were counted, analyzed, and photographed. The slide location of each metaphase was recorded so that they could be relocated following the autoradiography. The slides were dipped in NTB-3 liquid emulsion (Kodak) for 1 to 2 seconds. After 2 weeks of exposure at 4°C to a dry atmosphere, the slides were developed in D-19 (Kodak) for 4 minutes at 18°C. The autoradiographs corresponding to the previously selected well-spread metaphases were relocated and photographed. Photomicrographs of autoradiographs and the corresponding set of chromosomes were analyzed comparatively. Heavy grain content following autoradiography may make obscure chromosomal morphology, especially the fine structure, and make the exact identification of chromosomes difficult. In addition, the preparation of karyotypes before autoradiography introduces an element of objectivity into the matching of the corresponding chromosomes after autoradiography. Most importantly, the statements regarding light or heavy labeling of a chromosome and the chronology of chromosomal replication are based on the matching of the autoradiographs with previously determined karyotypes of the metaphases examined.View full abstractDownload PDF (1301K)
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