The chromosome structures of eight strains of T. araraticum collected in southeastern Turkey and Northern Iraq were analyzed by hybridization with three chromosome type tester strains; the A type of araraticum 196-1, the B type of timopheevi 107-1 and the C type of araraticum 1908A. Concurrently, the chromosome pairings of hybrids of eight araraticum strains were observed. Results of the present experiment clearly show that of the eight strains studied, four were the B type. The remaining four strains were classified into three chromosome types different from the A, B and C types. These were designated the D, E and F types. A tetravalent was formed in the PMCs of hybrids between the B and D types and between the B and E types. A hexavalent was observed in hybrids between the D and E types, between the E and F types and between the B and F types. Two tetravalents were recognized in hybrids between the A and D types and between. the C and E types. A hybrid between the D and F types showed an octavalent formation at meiosis. Both a tetravalent and a hexavalent were observed in a hybrid between the A and F types. Reduced seed fertility in hybrids of the strains of T. araraticum seems to be caused by meiotic irregularities such as polyvalent formations. The seed fertilities of hybrids between the B type strains of T. araraticum and T. timopheevi varied from almost sterile to fully fertile. An araraticum strain collected near Hozat, Turkey, was found to be fully fertile when crossed with T. timopheevi. This provides evidence for the theory that T. araraticum is the ancestral species of cultivated T. timopheevi. This study has clearly shown that chromosome differentiation in T. araraticum is more abundant in Northern Iraq than in Transcaucasus. This would indicate that the Timopheevi group of tetraploid wheats first originated in the eastern part of the Fertile Crescent and that later its distribution area was extended northward to the Transcaucasus.
It has been demonstrated by polyacrylamide gel electrophoresis and gel filtration on Ultrogel AcA 34 that rabbit antibody elicited to crucian carp hexose 6-phosphate dehydrogenase cross-reacts with starfish hexose 6-phosphate dehydrogenase. This supports our previous assumption that vertebrate and starfish hexose 6-phosphate dehydrogenases are homologous molecules.
A total of 21 newly found mutants of the housefly, Musca domestia L., were described, and revised linkage maps based on the linkage data obtained from this study and supplemented with data by other investigators were prepared and presented. Sixteen marker loci for the first linkage group, 14 loci for the second linkage group, 17 loci for the third linkage group, 7 loci for the fourth linkage group and 6 loci for the fifth linkage group were plotted on the maps.
Semen of the black rat was inseminated artificially into the female Norway rat by routine technique. Among 20 rats, 9 showed pregnancy. Five impregnanted rats were dissected on 8 to 14 days after insemination and one to 4 embryos were found. No offspring were born in the case of the artificial insemination. In the control in which the semen of the Norway rat was inseminated artificially into the female Norway rat, pregnancies were obtained at high frequency and offspring were successfully born as in natural matings. Chromosomes of the hybrid blastocysts showed 42 chromosomes consisting of two haploid sets, one from the Norway rat and the other from the black rat.
Synthesized amphiploid strains derived from an intermediate punctata-eichingeri strain (2x, CC, W1525)×O. punctata (2x, W1514) and O. punctata (2x, W1515)×O. collina (2x, CC, W0006) were crossed with strains having the BBCC genomes. The number of bivalent per cell at MI of the tetraploid F1 hybrids (KB-lines) ranged from 20 to 24 with a mean of 22.3 or 23.3. This suggests that the diploid punctata strains served as the donor of the B genome for the species having the B and C genomes.
The biological effects of panfuran-troche containing acetylfuratrizine were investigated. λ phages were induced from both Escherichia coli W3110(λ) and W3110(λindS). Radiosensitive mutants, E. coli NG30 and Hs30R were more sensitive for killing than the wild-type, H/r30R, to this medicine. Revertants (argF-→argF+) were detected in both H/r30R and Hs30R(uvrA-), but in NG30(recA-). The mutation frequency was much significant in Hs30R in comparison with that of H/r30R.