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Volume 34, Issue 8
Displaying 1-4 of 4 articles from this issue
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Akio AWA1959 Volume 34 Issue 8 Pages 245-252
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSThe present paper deals with some cytological effects of triethylene thio-phosphoramide (thio-TEPA or 'Tespamin', SUMITOMO Co.), a derivative of ethyleneimine compounds, upon tumor cells of the transplantable ascites tumors of the rat (MTK-sarcoma III) and mouse (EM-tumor), with particular regard to mitotic abnormalities induced by the drug. Intraperitoneal injections of the drug at dose levels of 1mg/kg, 2mg/kg and 5mg/kg were made in tumor-bearing animals on the 4th day of transplantation.
By the application at a dose of 1mg/kg in the MTK-sarcoma III, thio-TEPA exerted a destructive influence upon interphase cells with a considerable increase of cell-volume and morphological deformation in various ways. At the same time, there was a marked decrease in mitotic rate of the tumor cells. Predominant patterns of abnormalities induced in tumor cells are: the formation of micro-nuclei and multi-lobated nuclei, the reduction in stainability of the cytoplasm and nucleus, the swelling of the cytoplasm, and the formation of vacuoles in the cytoplasm.
Damaging effects of the drug on the chromosomes were also remarkable. Fragmentation and translocation of chromosomes were rather common at metaphase. In addition, the formation of bridges and heavy stickiness of chromosomes were usual in treated tumor cells at ana- and telophase. Basing on the results of present study by means of the Feulgen reaction, it is suggested that the mitotic abnormalities induced by the drug may be caused by the disturbance of the nucleic acid metabolism in the interphase nucleus.
It was found that some of tumor stem-cells infiltrated in the omentum have remained alive unaffected by the drug. It seems probable that they form a primary source for regrowth of the tumor by their proliferation.View full abstractDownload PDF (1258K) -
Masanaka TERADA, Isamu KONDO1959 Volume 34 Issue 8 Pages 253-260
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDNA was extracted and purified from the temperate phage a1 liberating from Salmonella paratyphi A 1015.
On the other hand, active phage suspension was prepared with another temperate phage a10s which is serologically unrelated with the above phage and liberate from Salmonella paratyphi A Komatsubara strain.
The mixed infection of S. gallinarum Akita strain with these noninfective a1 phage DNA and active a10s phage suspension was carried out on solid agar medium.
The new type phages just corresponding the recombinant type between both phage strains could be observed among the phage progeny from this mixed infection in proportion of about 10-4-10-5.
So far examined, these new type phages seem to be produced as the result from an interaction between the DNA strand injected from the intact phage a10s and the strand or rather the fragment of a1 DNA somehow entering into the same infected bacterial cells. In addition, one of these new types was revealed to possess the ability of lysogenic conversion concerning salmonella O(I) antigen originally provided in the donor phage of a1 DNA but not in phage a10s.View full abstractDownload PDF (1460K) -
Taro FUJII1959 Volume 34 Issue 8 Pages 261-267
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (1954K) -
Michio TSUKAMURA1959 Volume 34 Issue 8 Pages 268-273
Published: 1959
Released on J-STAGE: May 21, 2007
JOURNAL FREE ACCESSDownload PDF (526K)
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