The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Volume 48, Issue 3
Displaying 1-9 of 9 articles from this issue
  • VI. CHROMOSOME PAIRING IN THE INTERSPECIFIC HYBRIDS BETWEEN O. OFFICINALIS AND ITS RELATED DIPLOID SPECIES
    TSUGUFUMI OGAWA, TAIRA KATAYAMA
    1973 Volume 48 Issue 3 Pages 159-165
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    In order to elucidate the relationship among diploid species in the O. officinalis complex, 25 F1 hybrids between O. officinalis and its related species of strains (Intermediate, Ceylonese officinalis and O. eichingeri) were studied cytologically.
    Pollen was almost completely sterile in these hybrids, but occasionally some fertile pollens were produced. At diakinesis and MI of PMCs in all the F1 hybrids, 12 compactly paired bivalents were observed in most cases. On the other hand, crosses between O. officinalis and diploid O. punctata were unsuccessful, though more than two thousand spikelets were cross-pollinated.
    Based on these results, the authors postulated that the genome of Intermediate, O. eichingeri and the Ceylonese officinalis is homologous with that of O. officinalis and that there may be distinct reproductive barriers between diploid O. punctata and its related species or strains.
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  • YOSHIKO N. TOBARI, DAIGORO MORIWAKI
    1973 Volume 48 Issue 3 Pages 167-173
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Drosophila ananassae from six geographical localities in Southeast Asia were examined in the recombination frequencies in the male. The investigation reveals that male crossing-over may be very common. At least in the second chromosome from these populations, almost all males can be expected to carry some genetic elements to cause male recombination, although in general the recombination value in the male was not always so high as that in the female.
    The equality of complementary crossover classes in each family was observed suggesting the meiotic origin of the event.
    Positive correlation between recombination and k values was obtained.
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  • AKIO KUSANAGI
    1973 Volume 48 Issue 3 Pages 175-183
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Multiples in three different interchanges of Luzula elegans (syn. L. purpurea) with holokinetic chromosomes and post-reductional meiosis revealed the constant preferential orientation at second metaphase. Alternate orientation was common in interchange multiples contain unequal size interchange chromosomes and led to less reduced or almost complete fertility. In interchange multiple containing equal size interchange chromosomes, alternate and adjacent orientations occurred with about equal frequency and led to usually approximately 50 per cent fertility. It is suggested that the equilibrate orientation along the equator at second metaphase may be neccessary for orderly segregation at second metaphase. The equilibrate orientation along the second metaphase equator of interchange multiples containing interchange chromosomes of unequal size is alternate orientation. On the contrary, the equilibrate orientation in interchange multiples containing interchange chromosomes of equal size is either alternate or adjacent orientation. It is therefore true that such preferential orientation observed in interchange multiples containing unequal size interchange chromosomes of Luzula depends on (1) identical size and shape of original chromosomes, and on (2) holokinetic activity of chromosome per se.
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  • I. INDUCTION, AND BIOCHEMICAL AND GENETIC CHARACTERS
    SYUICHI DOI, TOSHIAKI TAKAHASHI, NAOHIKO YANAGISHIMA
    1973 Volume 48 Issue 3 Pages 185-195
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Large cell mutants were produced with high frequency when a haploid strain of Saccharomyces cerevisiae was cultured on a nutrient agar containing 500mg/l α-naphthaleneacetic acid (NAA). These mutants had no selective advantage in the presence of NAA. Biochemical and genetic analyses showed that most of these variants involve changes in genome structure.
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  • TOHRU SHIBUYA, YUKIAKI KURODA
    1973 Volume 48 Issue 3 Pages 197-205
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Chondrocytes of leg bone distal cartilages of 8-, 10- and 12-day normal (+/+) and Creeper (Cp/+) embryos were cultured in replicate culture tubes, in clonal culture dishes, and in rotation flasks. In replicate cultures, chondrocytes from Cp/+ embryos showed a more decreased growth rate than those from +/+ embryos. In clonal cultures, the higher percentage of cartilage-making cell colonies was obtained for Cp/+genotype than for +/+ genotype. In rotation cultures, chondrocytes from Cp/+embryos formed aggregates which were smaller in size and stronger in metachromatic staining than those from +/+ embryos. These results suggest that chondrocytes from Cp/+ embryos may differentiate more precociously than those from +/+ embryos, and that Cp gene may have effects in accelerating the differentiation and in suppressing the proliferation of chondrocytes.
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  • VI. STERILE AMPHIPLOID, sativa-australiensis (AAEE)
    YOSHIO WATANABE, KYO WAKASA
    1973 Volume 48 Issue 3 Pages 207-216
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    A new amphiploid, sativa-australiensis (AAEE, 2n=48) was successfully synthesized by treating the young shoots with 0.05 % colchicine solution which were derived from the dormant buds on the stems of the F1 hybrid. In MI of PMC's in F1, some bivalents were observed. The modal chromosome configuration was 2II+20I. In amphiploid, most of PMC's examined formed the regular pairing of 24II. The remarkable difference was shown in chromosome size of amphiploid as well as F1. From the frequency of occurrence of heteromorphic bivalents in F1, it was infered that there are some homology between the chromosomes of O. sativa and O. australiensis. F1 ybrid was completely sterile. Amphiploid induced was also sterile, although it formed anthers seemingly normal in shape. At the stage immediately after tetrad, the fusion of microspores was observed as the result of the failure of cell wall formation. Multi-germ-pores were also observed on the pollen grains developed from the isolated microspores as well as the fused ones.
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  • TSUNEYUKI YAMAZAKI, PETER E. THOMPSON
    1973 Volume 48 Issue 3 Pages 217-229
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    The SD-bw line, which was produced by recombination of SD-72 with a cn bw stock, shows fairly high SD activity (K>0.9). This line was irradiated with a high dose of X-rays and many chromosome rearrangements were produced. The SD activity was significantly reduced in many of the irradiated lines: in some cases, the original high activity was recovered in a few generations, while, in other cases, the reduction was permanent. The nature of the chromosome rearrangements was determined through recombination tests and cytological observation of the salivary gland chromosomes, and the results compared with the SD activity. The findings from these experiments are as follows:
    1. Only chromosome pairing in the SD region corresponding to the small pericentric inversion of SD lines is essential for the normal function of the SD gene.
    2. The Y chromosome may possess some function necessary for the SD gene to operate.
    3. There may be modifiers of SD activity on the third chromosome and/or the fourth chromosome.
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  • TAKEO MARUYAMA
    1973 Volume 48 Issue 3 Pages 231-234
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
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  • KOICHI YAMAMOTO, YOSHIO OJIMA
    1973 Volume 48 Issue 3 Pages 235-238
    Published: 1973
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
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