The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
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Volume 54, Issue 6
Displaying 1-3 of 3 articles from this issue
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  • UNSCHEDULED DNA SYNTHESIS IN THE GERM CELLS OF MALE MICE IN VIVO
    NORIHO TANAKA, MOTOE KATOH
    1979 Volume 54 Issue 6 Pages 405-414
    Published: 1979
    Released on J-STAGE: May 21, 2007
    DOIhttps://doi.org/10.1266/jjg.54.405
    JOURNAL FREE ACCESS
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    Unscheduled DNA synthesis (UDS) was studied by a quantitative autoradiographic technique in vivo in the germ cells of male mice after exposure to various mutagens. In the first experiment, we examined the most active cell stage for UDS in mouse spermatogenesis after treatment with methyl methanesulfonate (MMS) or iso-propyl methanesulfonate (iPMS). The UDS in the male germ cells reached plateau level within 2-6 hours after treatment. The activity of UDS induced by the chemicals was highest in early spermatids per DNA content, and in the late pachytene stage per cell among the various stages of germ cells. In the second experiment, 10 compounds, mostly known chemical mutagens, were tested at different doses. The numbers of grains in diakinesis and metaphase-I (dia and M-I) were counted 2 days after treatment. Although MMS, 4-hydroxyaminoquinoline-1-oxide (4-HAQO), ethyl methanesulfonate (EMS) and iPMS induced strong UDS in this order, Mitomycin C (MC), 2-acetylaminofluorene (2-AAF), m-phenylenediamine (mPDA) and dimethyl nitrosoamine (DMNA) did not clearly induce UDS. Furylfuramide (AF-2) and busulfan had only slight effects.
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  • X-RAY-INDUCED CELL KILLING AND MUTATIONS IN CULTURED HUMAN CELL LINES (XERODERMA PIGMENTOSUM CELLS AND HELA S3 CELLS)
    YOSHIHIRO MURAI, SACHIKO TATSUKAWA, MASAKATSU HORIKAWA
    1979 Volume 54 Issue 6 Pages 415-426
    Published: 1979
    Released on J-STAGE: May 21, 2007
    DOIhttps://doi.org/10.1266/jjg.54.415
    JOURNAL FREE ACCESS
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    This paper deals with the development of assay systems for radiation-induced mutations to 8-azaguanine (8AG) resistance, using XP2OS cells which belong to complementation group A of xeroderma pigmentosum (XP) and the familiar human cancer HeLa S3 cells. The results indicated that these mutation assay systems to purine analogue resistance using XP2OS and HeLa S3 cells are useful for determining the mutation frequency induced by X-rays and that the data obtained by using these assay systems can be used for predicting the mutation frequency expected after exposure of man to low doses of ionizing radiation. In addition, a plot of induced mutation frequency against log surviving fraction yielded an approximately linear relationship for five cell types including human diploid cells, Chinese hamster cells and mouse lymphoma cells, which have already been studied by other workers. This relationship seems to suggest that mammalian cells generally have the same fixed probability of mutation induction relative to the extent of damage caused by ionizing radiation.
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  • THE INTERACTION OF METABOLIC SYSTEMS WITH DAMAGED DNA
    BERNARD S. STRAUSS
    1979 Volume 54 Issue 6 Pages 489-512
    Published: 1979
    Released on J-STAGE: May 21, 2007
    DOIhttps://doi.org/10.1266/jjg.54.489
    JOURNAL FREE ACCESS
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