The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Volume 51, Issue 4
Displaying 1-8 of 8 articles from this issue
  • NOBUMICHI INOMATA, MASASUKE OKAMOTO, HITOSHI ASAMI
    1976 Volume 51 Issue 4 Pages 223-228
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Behavior of unstable lines of telocentric chromosomes in cultured callus cells was investigated. Callus tissues were induced from the germinated seeds in the medium of RM-64 with 3.0mg/l of 2, 4-C. Short telos of 3BS1 were most unstable. In the 3BS1 line the cells with less than diploid chromosome number were more unstable than those above diploid chromosome number. Long telos of 3BS8 were more stable than those of 3BS1 and 3BS4. Different stabilities in callus tissues were observed between the line of 3BS4 and 3BS8, although stabilities of 3BS4 and 3BS8 were almost the same under natural condition.
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  • YUZURU OGUMA
    1976 Volume 51 Issue 4 Pages 229-236
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    A new kind of inversion, named In2RB, has been found in the Engaru population of Anopheles sinensis. This is a simple inversion located at 9A-11D on the right arm of the second chromosome. Inversion polymorphism was investigated on In2RA, In2RB and In3RA in material from 11 localities. In2RA was not observed at all the localities and In2RB was found only in the Engaru population. In3RA was observed in four strains with low frequency. The hybrid chromosomes obtained from crosses between the Engaru strain and either the Kanoya or the Yakumo strains showed incomplete synapsis along the whole lengths of the chromosome arms.
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  • I. RADIATION- AND CHEMICAL-INDUCTION OF NUTRITIONALLY DEFICIENT AND SUFFICIENT MUTANTS IN CHINESE HAMSTER HAI CELLS IN VITRO
    SADAYUKI BAN, FUMIO SUZUKI, MASAKATSU HORIKAWA
    1976 Volume 51 Issue 4 Pages 237-251
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    A forward mutation system using prototrophic CH-hai Cl 23 cells (Ala+, Asn+, Pro+, Asp+, Hyp+, Glu+) isolated from an original Chinese hamster hai cell line was found to be much more sensitive than a reverse mutation system using auxotrophic CH-hai Cl 3 cells (TdR-), for detecting the mutations induced by chemical carcinogens or mutagens (4-nitroquinoline 1-oxide, N-methyl-N'nitro-N-nitrosoguanidine, ethyl methane-sulfonate), as well as by X-rays and UV. It was found that the antimicrobial food additive, 2-(2-furyl)-3-(5-nitro-2-furyl) acrylic acid amide (furylfuramide: FF or AF2) has highly mutagenic activity so far as it was tested by the forward mutations. The forward mutation system, from prototrophic CH-hai Cl 23 cells to auxotrophs, established in this study can be applied as a useful system for detecting environmental mutagens and for elucidating the mechanisms of somatic cell mutation.
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  • II. RADIATION-INDUCTION OF 8-AZAGUANINE-RESISTANT AND -SENSITIVE MUTANTS IN CHINESE HAMSTER HAI CELLS IN VITRO
    MASAKATSU HORIKAWA, FUMIO SUZUKI, SADAYUKI BAN
    1976 Volume 51 Issue 4 Pages 253-264
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    In order to establish a sensitive assay system for the mutagens and to understand the nature of the differences in the mutation frequency among various somatic mammalian cell lines at various genetic markers reported by many workers, we examined the frequency of the forward and reverse mutations induced by X- and UV-irradiation, by using 8-azaguanine (8AG)-sensitive cells (prototrophic CH-hai Cl 23 cells) isolated from an original Chinese hamster hai cell line and 8AG-resistant cells isolated from CH-hai Cl 23 cells. However, both the mutation systems, especially the forward mutation system used here, were less sensitive as the assay system of the mutations induced by X-rays or UV, compared with the nutritional forward mutation system used previously (Ban et al. 1976). These results underline the complexity of the study of mutagenesis using cultured mammalian cells, particularly because the induced mutation frequency in cultured cells is affected by the factors of the mutation expression time, the size of the cell inoculum, the concentration of the selective agent, etc.
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  • AKISHI FUKATAMI
    1976 Volume 51 Issue 4 Pages 265-276
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Chromosomal polymorphism in natural populations of Drosophila lutescens, a semidomestic species, were studied. Chromosomal variations in six natural populations and thirty local strains maintained in laboratory culture were examined. In all populations remarkable inversion polymorphism was observed in four arms of the second and the third chromosomes. It was shown that there were clinal changes in the frequency of some gene arrangements (2L A, 3L A, 3L C and 3R D) from north-east to south-west in Japan. It is assumed that the main factor controlling the inversion frequencies is temperature.
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  • YASUSHI TAKENOUCHI
    1976 Volume 51 Issue 4 Pages 277
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
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  • YASUSHI TAKENOUCHI
    1976 Volume 51 Issue 4 Pages 279-283
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    The chromosomes of four Catapionus gracilicornis male were studied. Three had the ordinal basic number 10AA+Xyp in haploid (2n=22) and remaining one, 11AA+Xyp (2n=24). The detailed investigation revealed that the latter chromosome formula was brought about by Robertsonian fission.
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  • MASAO KIMURA
    1976 Volume 51 Issue 4 Pages 285-288
    Published: 1976
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
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