The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
Volume 47, Issue 4
Displaying 1-10 of 10 articles from this issue
  • MASATOSHI ENOMOTO
    1972 Volume 47 Issue 4 Pages 227-235
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Chlorate-resistant mutants (chl-) were isolated from Salmonella typhimurium on the nutrient medium containing glucose and KClO3 by the incubation under anaerobic condition. Phenotypic characterization and linkage analyses by transduction with phage P22 and mating with an Hfr strain, SW1391, demonstrated that the chl mutants can be classified at least into three groups and the corresponding genetic loci are mapped in the order trp-chlC-chlB-chlA-bio-gal, in which only chlA is cotransducible with bio and gal. The chlC mutants showed no growth on a minimal medium, which was weakly restored by the addition of NAD (nicotinamide-adenine dinucleotide) or a commercial vitamin complex. Other pleiotropic mutants requiring lysine and methionine or NAD and those requiring cystein and methionine or NAD for growth on the minimal medium were isolated. Nitrate reductase activity was higher and more stable in the insoluble extract than the soluble one, indicating that this enzyme is membrane-bound.
    Download PDF (1007K)
  • I. COMMERCIAL LOWLAND-RICE CULTIVATED IN JAPAN
    CHOYU SHINJYO
    1972 Volume 47 Issue 4 Pages 237-243
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    One hundred and fifty lowland-rice varieties recommended by forty-seven prefectural authorities of Japan in 1962 were crossed with two different testers in order to screen male sterility-inducing cytoplasm and fertility-restoring genes.
    Nineteen varieties (12.7%) carrying weak fertility-restoring gene(s) and 131 non-carriers (87.3%) were found. Almost all varieties carrying the weak restoring gene(s) were concentrated in southern Japan (Kyushu, Shikoku, Chugoku and Kinki Districts) and were developed by pure-line selection from their native varieties.
    As to cytoplasm type, male sterility-inducing cytoplasm such as that found in Chinsurah Boro II was not found. Namely, all varieties tested possessed a normal cytoplasm.
    Download PDF (553K)
  • HIDEJIRO TAKAHASHI
    1972 Volume 47 Issue 4 Pages 245-248
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Using Petunia hybrida, it was attempted to produce new S alleles by selfing.
    The inbred progenies which were self-incompatible were reciprocally crossed with their parents. As a result no new S specificity was produced, but a part of the progenies showed various grades of fertility only when they were used as the female. Such unilateral pseudo-compatibility was not observed between the hybrid progenies and their parents.
    An interpretation for the phenomenon‘unilateral pseudo-compatibility’was given.
    Download PDF (295K)
  • KUNIAKI WATANABE, YOSHIAKI NISHII, RYUSO TANAKA
    1972 Volume 47 Issue 4 Pages 249-255
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Calluses were induced with 100% rate from anthers of 8 species and 2 varieties of diploid and polyploid Chrysanthemum species.
    Most of the calluses were found to be derived from the cells of the connective tissue between the thecae of the anthers. Within 40 days from cultivation, these calluses differentiated roots, and numerous shoots were differentiated within 40 days after transplanting to the medium without auxin hormones.
    The induced plants examined in some genetic strains showed the same chromosome number as the respective mother strains.
    Download PDF (1515K)
  • YUICHI TANABE, TERUAKI ISE
    1972 Volume 47 Issue 4 Pages 257-263
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Agar gel electrophoresis of chicken liver extracts revealed the presence of four regions of esterase activity, which were designated I, II, III and IV. Electrophoretic variants of esterases in Region I, which were classified as arylesterase, were shown to be controlled by one autosomal Es-4 locus with three multiple alleles Es-4A, Es-4B and Es-4O. The alleles, Es-4A and Es-4B, are codominant, while Es-4O is recessive to the two codominant alleles. Genetic variation was found in esterases in Region IV which were classified as acetylesterase. These variants in Region IV were shown to be controlled by two codominant alleles Es-6A and Es-6B on one autosomal locus. Organ-specific differences in esterase isozyme patterns were also observed.
    Download PDF (1684K)
  • TAKAJI IKUSHIMA
    1972 Volume 47 Issue 4 Pages 265-275
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Seeds of a genetic stock of diploid oats (2n=14) which is heterozygous for the plant color (al/+) were presoaked for periods ranging from 0 to 48 hours and then exposed to X rays or 14.1MeV fast neutrons. The RBE of 14.1MeV fast neutrons for the reduction of seedling height was 11.5 in dry seeds, while much lower RBEs of 2.4, 1.9, 1.3 and 1.2 were obtained from 6, 12, 24 and 48 hour presoaked seeds, respectively. The RBE obtained from dry seeds for the induction of somatie mutations was 24, but the corresponding values were 4.0, 2.3, 1.9, and 1.7 in 6, 12, 24 and 48 hour presoaked seeds, respectively. Decrease of RBE values in wet seeds was thus clearly demonstrated and lower values are close to those from other organisms than to those from dry seeds of higher plants. The results show clearly that water content is one of the major modifying factors of RBE values. Other possible modifying factors were also discussed.
    Download PDF (955K)
  • KOICHIRO TSUNEWAKI, YASUO NAKAI
    1972 Volume 47 Issue 4 Pages 277-290
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    About 600 cultivars or strains of wheat, collected from Mediterranean countries, including the USSR, and classified as Triticum aestivum, T. compactum, T. spelta, or T. macha of common wheat, were studied for necrosis and chlorosis genotypes by crossing them to three testers of known genotypes. On the basis of the results, the geographical distributions of Ne1 and Ne2 genes for necrosis and Ch1 and Ch2 genes for chlorosis were clarified.
    For necrosis genes, the western border of the Asian type population, in which Ne1-carriers predominate over Ne2-carriers, extended from Egypt to the Caucasus through Turkey. Between two areas occupied by the Asian type population and the Western type population, in which Ne2-carrier are dominant over Ne1-carriers, transitional type populations containing both Ne1- and Ne2-carriers at moderate frequencies were found. This transitional zone covers Portugal, Spain, Italy and the USSR (excluding the Caucasus). Two non-carriers regions, where frequencies of both Ne1 and Ne2-carriers were very low, were found in this area. These are the Balkan Peninsula (Yugoslavia, Rumania, Bulgaria, Greece and Western Turkey) and the Near East (South Turkey, Syria, Lebanon and Jordan). The latter is, however, not definitive, because of the small number of materials examined. An Ne1-carrier region, where the frequency of Ne1 gene is extremely high (60-99%), was found in the North Iran-Armenia-Central Turkey region.
    For chlorosis genes, the common wheat populations of all countries investigated showed extremely high frequencies of Ch2-carriers (88-100%). No Ch1-carrier was found, except two collections of T. macha from the Caucasus. Thus, the Mediterranean population of common wheat is the same as those in other parts of the world.
    Download PDF (1195K)
  • YASUSHI TAKENOUCHI, NAOTO MURAMOTO
    1972 Volume 47 Issue 4 Pages 291-295
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    The chromosomes of Orthocephalus funestus Jakovlev (Miridae: Heteroptera) were studied in male germ cells according to the acetic orcein squash method. The chromosomes of the species are 2n=30, MI, 16, MII, 15 and the sex-determining system is of the X-Y type.
    Fragment chromosomes were observed in four bugs (5.3%) out of seventy-six in 1970, while the other seventy-two bugs (94.7%) had normal chromosome complements. The number of the first-spermatocytes observed in the remaining four bugs is one hundred and twenty-six. The chromosome complements of seventy-six cells (60%) out of one hundred and twenty-six are normal and the remaining 50 cells (40%) have fragment chromosomes.
    Download PDF (595K)
  • SHIGEKATSU TSUJI, HIROSHI MATSUSHITA
    1972 Volume 47 Issue 4 Pages 297-299
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Download PDF (575K)
  • MASAO KIMURA
    1972 Volume 47 Issue 4 Pages 301-304
    Published: 1972
    Released on J-STAGE: May 21, 2007
    JOURNAL FREE ACCESS
    Egg white proteins of the black-tailed gull (Larus crassirostris) were resolved into four regions by starch gel electrophoresis. Region IV consisted of three zones of protein which migrated from sample insertion slot to the cathode in the pH 7.5 gel. Proteins in region IV were identified as conalbumins. Three types of electrophoretic pattern of conalbumin were detected.
    Download PDF (815K)
feedback
Top